FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149374499> ?p ?o ?g. }

• W2149374499 endingPage "25" @default.
• W2149374499 startingPage "25" @default.
• W2149374499 abstract "Western clover (Trifolium occidentale) is a perennial herb with characteristics compatible for its development as an attractive model species for genomics studies relating to the forage legume, white clover (Trifolium repens). Its characteristics such as a small diploid genome, self-fertility and ancestral contribution of one of the genomes of T. repens, facilitates its use as a model for genetic analysis of plants transformed with legume or novel genes. In this study, a reproducible transformation protocol was established following screening of T. occidentale accessions originating from England, Ireland, France, Spain and Portugal. The protocol is based upon infection of cotyledonary explants dissected from mature seed with the Agrobacterium tumefaciens strain GV3101 carrying vectors which contain the bar selection marker gene. Transformation frequencies of up to 7.5% were achieved in 9 of the 17 accessions tested. Transformed plants were verified by PCR and expression of the gusA reporter gene, while integration of the T-DNA was confirmed by Southern blot hybridisation and segregation of progeny in the T1 generation. Development of this protocol provides a valuable contribution toward establishing T. occidentale as a model species for white clover. This presents opportunities for further improvement in white clover through the application of biotechnology." @default.
• W2149374499 created "2016-06-24" @default.
• W2149374499 creator A5015825469 @default.
• W2149374499 creator A5020417012 @default.
• W2149374499 creator A5031640938 @default.
• W2149374499 creator A5084248810 @default.
• W2149374499 date "2013-01-01" @default.
• W2149374499 modified "2023-09-26" @default.
• W2149374499 title "Genetic transformation of western clover (Trifolium occidentale D. E. Coombe.) as a model for functional genomics and transgene introgression in clonal pasture legume species" @default.
• W2149374499 cites W1598508848 @default.
• W2149374499 cites W1972835183 @default.
• W2149374499 cites W1980243156 @default.
• W2149374499 cites W1984261370 @default.
• W2149374499 cites W1985355665 @default.
• W2149374499 cites W1987355768 @default.
• W2149374499 cites W1994470176 @default.
• W2149374499 cites W2001710923 @default.
• W2149374499 cites W2002523169 @default.
• W2149374499 cites W2013507874 @default.
• W2149374499 cites W2020456518 @default.
• W2149374499 cites W2024556021 @default.
• W2149374499 cites W2028903821 @default.
• W2149374499 cites W2032041269 @default.
• W2149374499 cites W2037512861 @default.
• W2149374499 cites W2052227130 @default.
• W2149374499 cites W2053889679 @default.
• W2149374499 cites W2061168807 @default.
• W2149374499 cites W2064051161 @default.
• W2149374499 cites W2065035179 @default.
• W2149374499 cites W2074308771 @default.
• W2149374499 cites W2076604597 @default.
• W2149374499 cites W2077545872 @default.
• W2149374499 cites W2092030185 @default.
• W2149374499 cites W2094433601 @default.
• W2149374499 cites W2110630756 @default.
• W2149374499 cites W2133592536 @default.
• W2149374499 cites W2139448591 @default.
• W2149374499 cites W2141607299 @default.
• W2149374499 cites W2163737871 @default.
• W2149374499 cites W2168875816 @default.
• W2149374499 cites W2178364949 @default.
• W2149374499 cites W2323221187 @default.
• W2149374499 cites W289045332 @default.
• W2149374499 cites W74292535 @default.
• W2149374499 doi "https://doi.org/10.1186/1746-4811-9-25" @default.
• W2149374499 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3716983" @default.
• W2149374499 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23841995" @default.
• W2149374499 hasPublicationYear "2013" @default.
• W2149374499 type Work @default.
• W2149374499 sameAs 2149374499 @default.
• W2149374499 citedByCount "7" @default.
• W2149374499 countsByYear W21493744992014 @default.
• W2149374499 countsByYear W21493744992015 @default.
• W2149374499 countsByYear W21493744992020 @default.
• W2149374499 countsByYear W21493744992023 @default.
• W2149374499 crossrefType "journal-article" @default.
• W2149374499 hasAuthorship W2149374499A5015825469 @default.
• W2149374499 hasAuthorship W2149374499A5020417012 @default.
• W2149374499 hasAuthorship W2149374499A5031640938 @default.
• W2149374499 hasAuthorship W2149374499A5084248810 @default.
• W2149374499 hasBestOaLocation W21493744991 @default.
• W2149374499 hasConcept C104317684 @default.
• W2149374499 hasConcept C141231307 @default.
• W2149374499 hasConcept C204241405 @default.
• W2149374499 hasConcept C2776632002 @default.
• W2149374499 hasConcept C2776702220 @default.
• W2149374499 hasConcept C2777732983 @default.
• W2149374499 hasConcept C54355233 @default.
• W2149374499 hasConcept C59822182 @default.
• W2149374499 hasConcept C8182607 @default.
• W2149374499 hasConcept C86803240 @default.
• W2149374499 hasConceptScore W2149374499C104317684 @default.
• W2149374499 hasConceptScore W2149374499C141231307 @default.
• W2149374499 hasConceptScore W2149374499C204241405 @default.
• W2149374499 hasConceptScore W2149374499C2776632002 @default.
• W2149374499 hasConceptScore W2149374499C2776702220 @default.
• W2149374499 hasConceptScore W2149374499C2777732983 @default.
• W2149374499 hasConceptScore W2149374499C54355233 @default.
• W2149374499 hasConceptScore W2149374499C59822182 @default.
• W2149374499 hasConceptScore W2149374499C8182607 @default.
• W2149374499 hasConceptScore W2149374499C86803240 @default.
• W2149374499 hasIssue "1" @default.
• W2149374499 hasLocation W21493744991 @default.
• W2149374499 hasLocation W21493744992 @default.
• W2149374499 hasLocation W21493744993 @default.
• W2149374499 hasLocation W21493744994 @default.
• W2149374499 hasOpenAccess W2149374499 @default.
• W2149374499 hasPrimaryLocation W21493744991 @default.
• W2149374499 hasRelatedWork W1952223398 @default.
• W2149374499 hasRelatedWork W1970480054 @default.
• W2149374499 hasRelatedWork W1981648165 @default.
• W2149374499 hasRelatedWork W2000713737 @default.
• W2149374499 hasRelatedWork W2003652887 @default.
• W2149374499 hasRelatedWork W2098030355 @default.
• W2149374499 hasRelatedWork W2557629578 @default.
• W2149374499 hasRelatedWork W3113603988 @default.
• W2149374499 hasRelatedWork W4234342413 @default.
• W2149374499 hasRelatedWork W82204743 @default.

• next