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- W2149393804 abstract "The chapter discusses the capillary isoelectric focusing. The practice of isoelectric focusing in the capillary format provides the high resolving power of conventional gel isoelectric focusing (IEF) and the automation capabilities of instrumental techniques such as capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC). The principle of capillary isoelectric focusing (cIEF) is similar to that of gel IEF: proteins migrate within a stable pH gradient formed by carrier ampholytes under the influence of an electric field. A major limitation of performing IEF in capillaries is the use of fixed-point optical detectors on most commercial CE systems. In this approach, a short section of the capillary serves as the “flow cell” for ontube detection. Sample preparation for cIEF includes adjustment of sample ionic strength and protein concentration. The quality of the cIEF separation will depend upon the range and complexity of the carrier ampholyte mixture. The final step in cIEF is mobilization of focused zones through the detection point. Two-step cIEF has been performed using electrophoretic and hydraulic mobilization. Hydraulic mobilization can be accomplished by applying pressure to the head space above the capillary inlet, by applying vacuum to the capillary outlet, or by elevating the inlet of the capillary relative to the outlet of the capillary. Monoclonal antibodies, which are widely used as diagnostic and therapeutic tools, often exhibit microheterogeneity." @default.
- W2149393804 created "2016-06-24" @default.
- W2149393804 creator A5056666361 @default.
- W2149393804 date "2005-01-01" @default.
- W2149393804 modified "2023-10-14" @default.
- W2149393804 title "9 Capillary isoelectric focusing" @default.
- W2149393804 cites W1889541544 @default.
- W2149393804 cites W1926033671 @default.
- W2149393804 cites W1949489872 @default.
- W2149393804 cites W1966628264 @default.
- W2149393804 cites W1967805658 @default.
- W2149393804 cites W1968114925 @default.
- W2149393804 cites W1971556344 @default.
- W2149393804 cites W1972604012 @default.
- W2149393804 cites W1974428670 @default.
- W2149393804 cites W1975086323 @default.
- W2149393804 cites W1978310073 @default.
- W2149393804 cites W1981643728 @default.
- W2149393804 cites W1982620548 @default.
- W2149393804 cites W1983344821 @default.
- W2149393804 cites W1991391288 @default.
- W2149393804 cites W1991989238 @default.
- W2149393804 cites W1992623933 @default.
- W2149393804 cites W1992749184 @default.
- W2149393804 cites W1997029544 @default.
- W2149393804 cites W1998209536 @default.
- W2149393804 cites W1998222484 @default.
- W2149393804 cites W2000199268 @default.
- W2149393804 cites W2002100812 @default.
- W2149393804 cites W2003896820 @default.
- W2149393804 cites W2005754127 @default.
- W2149393804 cites W2009233489 @default.
- W2149393804 cites W2010370005 @default.
- W2149393804 cites W2011509907 @default.
- W2149393804 cites W2015134178 @default.
- W2149393804 cites W2015379769 @default.
- W2149393804 cites W2015750060 @default.
- W2149393804 cites W2015883452 @default.
- W2149393804 cites W2018182272 @default.
- W2149393804 cites W2019912404 @default.
- W2149393804 cites W2020414951 @default.
- W2149393804 cites W2025091036 @default.
- W2149393804 cites W2025698368 @default.
- W2149393804 cites W2026232704 @default.
- W2149393804 cites W2028865017 @default.
- W2149393804 cites W2029023041 @default.
- W2149393804 cites W2029339919 @default.
- W2149393804 cites W2030908395 @default.
- W2149393804 cites W2036453908 @default.
- W2149393804 cites W2036761507 @default.
- W2149393804 cites W2037708145 @default.
- W2149393804 cites W2042738392 @default.
- W2149393804 cites W2043229877 @default.
- W2149393804 cites W2044690556 @default.
- W2149393804 cites W2045179012 @default.
- W2149393804 cites W2046557218 @default.
- W2149393804 cites W2046586856 @default.
- W2149393804 cites W2047831402 @default.
- W2149393804 cites W2048215036 @default.
- W2149393804 cites W2049748110 @default.
- W2149393804 cites W2051039769 @default.
- W2149393804 cites W2055038936 @default.
- W2149393804 cites W2055158187 @default.
- W2149393804 cites W2056709373 @default.
- W2149393804 cites W2059199466 @default.
- W2149393804 cites W2060917646 @default.
- W2149393804 cites W2061419992 @default.
- W2149393804 cites W2065724260 @default.
- W2149393804 cites W2065970415 @default.
- W2149393804 cites W2070581811 @default.
- W2149393804 cites W2070709997 @default.
- W2149393804 cites W2070808569 @default.
- W2149393804 cites W2071150425 @default.
- W2149393804 cites W2072787632 @default.
- W2149393804 cites W2075451726 @default.
- W2149393804 cites W2083842525 @default.
- W2149393804 cites W2084786239 @default.
- W2149393804 cites W2086268840 @default.
- W2149393804 cites W2088534740 @default.
- W2149393804 cites W2088694714 @default.
- W2149393804 cites W2089163089 @default.
- W2149393804 cites W2089593903 @default.
- W2149393804 cites W2090176404 @default.
- W2149393804 cites W2092602707 @default.
- W2149393804 cites W2093598543 @default.
- W2149393804 cites W2094333237 @default.
- W2149393804 cites W2095027198 @default.
- W2149393804 cites W2099960600 @default.
- W2149393804 cites W2100234353 @default.
- W2149393804 cites W2102128634 @default.
- W2149393804 cites W2137332836 @default.
- W2149393804 cites W2150283014 @default.
- W2149393804 cites W2157671028 @default.
- W2149393804 cites W2160949470 @default.
- W2149393804 cites W3148214213 @default.
- W2149393804 cites W1983089990 @default.
- W2149393804 doi "https://doi.org/10.1016/s0149-6395(05)80012-1" @default.
- W2149393804 hasPublicationYear "2005" @default.