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- W2149409785 abstract "The ferret is an excellent model for many human infectious diseases including influenza, SARS-CoV, henipavirus and pneumococcal infections. The ferret is also used to study cystic fibrosis and various cancers, as well as reproductive biology and physiology. However, the range of reagents available to measure the ferret immune response is very limited. To address this deficiency, high-throughput real time RT-PCR TaqMan assays were developed to measure the expression of fifteen immune mediators associated with the innate and adaptive immune responses (IFNα, IFNβ, IFNγ, IL1α, IL1β, IL2, IL4, IL6, IL8, IL10, IL12p40, IL17, Granzyme A, MCP1, TNFα), as well as four endogenous housekeeping genes (ATF4, HPRT, GAPDH, L32). These assays have been optimized to maximize reaction efficiency, reduce the amount of sample required (down to 1 ng RNA per real time RT-PCR reaction) and to select the most appropriate housekeeping genes. Using these assays, the expression of each of the tested genes could be detected in ferret lymph node cells stimulated with mitogens or infected with influenza virus in vitro. These new tools will allow a more comprehensive analysis of the ferret immune responses following infection or in other disease states." @default.
- W2149409785 created "2016-06-24" @default.
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- W2149409785 date "2014-11-01" @default.
- W2149409785 modified "2023-09-26" @default.
- W2149409785 title "Low levels of IL17A may protect filarial infected subjects from development of SLE and RA in an endemic area" @default.
- W2149409785 doi "https://doi.org/10.1016/j.injr.2014.10.219" @default.
- W2149409785 hasPublicationYear "2014" @default.
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