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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149430107> ?p ?o ?g. }

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• W2149430107 abstract "Here we report the development of our Next Generation Automated Multiplexed Oligonucleotide Synthesizer (NG-1536-AMOS), capable of producing 1536 samples in a single run using a multi-well filtered titer plate. With the potential to synthesize up to 3456 samples per plate, we converted the BioRAPTR Flying Reagent Dispenser into an open-well system where spent reagents are drained to waste under vacuum. During synthesis, reagents are delivered on-the-fly to each micro-titer well at volumes ≤5 μl with plate speeds up to 150 mm/s. Using gas-phase cleavage and deprotection, a full plate of 1536 60 mers may be processed with same-day turnaround with an average yield per well at 3.5 nmol. Final product at only $0.00277/base is eluted into a low-volume collection plate for immediate use in downstream application (e.g. Biomek FX for versatile sample handling). Also, crude oligonucleotide quality is comparable to that of commercial synthesis instrumentation, with an error rate on the NG-1536-AMOS platform of 1.53/717 bases. Furthermore, mass spectral analysis on strands synthesized up to 80 bases showed high purity with an average coupling efficiency of 99.5%." @default.
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• W2149430107 date "2014-02-01" @default.
• W2149430107 modified "2023-09-26" @default.
• W2149430107 title "Next generation 1536-well oligonucleotide synthesizer with on-the-fly dispense" @default.
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• W2149430107 doi "https://doi.org/10.1016/j.jbiotec.2013.11.027" @default.
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