Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149444911> ?p ?o ?g. }
- W2149444911 endingPage "33" @default.
- W2149444911 startingPage "4323" @default.
- W2149444911 abstract "Efficient gene transfer by recombinant adenovirus (Ad) vectors depends on expression of CAR and alpha(v) integrin on target cells. Because Ad may also infect nearby nontarget cells expressing these receptors, such as peritoneal mesothelial cells after i.p. injection, we hypothesized that targeting Ad gene delivery to a receptor overexpressed on most ovarian carcinoma cells, such as TAG-72, would enhance the selectivity of Ad gene transfer when used in this context. A monoclonal antibody that has been investigated clinically for immunotherapy and immunodetection of ovarian carcinomas, namely CC49, was used to construct a bispecific conjugate with the Fab fragment of a neutralizing anti-knob mAb to target Ad binding via TAG-72. This conjugate facilitated TAG-72-specific, CAR-independent Ad reporter gene transfer to both ovarian cancer cell lines and primary ovarian cancer cells cultured from malignant ascites fluid. Fab-CC49 was very selective for tumor cells, augmenting Ad gene transfer to primary ovarian cancer cells 2- to 28-fold relative to untargeted Ad, while also decreasing gene transfer to autologous cultured mesothelial cells 4- to 9-fold. These data suggest that targeting Ad via TAG-72 may improve the selectivity of Ad gene transfer for ovarian tumors 8- to 252-fold on i.p. vector injection. These results also define the requirements for a candidate target receptor in the rational design of a targeted Ad vector for ultimate clinical utility, one that selectively infects tumor cells and spares normal cells on i.p. injection. Such a vector may increase gene transfer and decrease the toxicity of Ad vectors, which would improve the therapeutic index of cytotoxic gene therapy for ovarian cancer in clinical trials." @default.
- W2149444911 created "2016-06-24" @default.
- W2149444911 creator A5009816072 @default.
- W2149444911 creator A5022987623 @default.
- W2149444911 creator A5026244234 @default.
- W2149444911 creator A5047657528 @default.
- W2149444911 creator A5050724475 @default.
- W2149444911 creator A5052212062 @default.
- W2149444911 creator A5080794406 @default.
- W2149444911 date "2000-11-01" @default.
- W2149444911 modified "2023-10-18" @default.
- W2149444911 title "Selectivity of TAG-72-targeted adenovirus gene transfer to primary ovarian carcinoma cells versus autologous mesothelial cells in vitro." @default.
- W2149444911 cites W1502767888 @default.
- W2149444911 cites W1533102977 @default.
- W2149444911 cites W1567424149 @default.
- W2149444911 cites W1807942412 @default.
- W2149444911 cites W1809188197 @default.
- W2149444911 cites W1942221917 @default.
- W2149444911 cites W1967364357 @default.
- W2149444911 cites W1975118160 @default.
- W2149444911 cites W1979273582 @default.
- W2149444911 cites W1979800392 @default.
- W2149444911 cites W1988876819 @default.
- W2149444911 cites W1991710882 @default.
- W2149444911 cites W1998128870 @default.
- W2149444911 cites W2010226282 @default.
- W2149444911 cites W2013237043 @default.
- W2149444911 cites W2025243162 @default.
- W2149444911 cites W2029709100 @default.
- W2149444911 cites W2031874876 @default.
- W2149444911 cites W2037383080 @default.
- W2149444911 cites W2051758487 @default.
- W2149444911 cites W2061055235 @default.
- W2149444911 cites W2065392104 @default.
- W2149444911 cites W2068855233 @default.
- W2149444911 cites W2089120635 @default.
- W2149444911 cites W2094136836 @default.
- W2149444911 cites W2103144075 @default.
- W2149444911 cites W2106448082 @default.
- W2149444911 cites W2107619042 @default.
- W2149444911 cites W2116071714 @default.
- W2149444911 cites W2119940209 @default.
- W2149444911 cites W2123927174 @default.
- W2149444911 cites W2125131695 @default.
- W2149444911 cites W2125923808 @default.
- W2149444911 cites W2137415320 @default.
- W2149444911 cites W2147772438 @default.
- W2149444911 cites W2147961945 @default.
- W2149444911 cites W2150110662 @default.
- W2149444911 cites W2158071204 @default.
- W2149444911 cites W2158691858 @default.
- W2149444911 cites W2163643581 @default.
- W2149444911 cites W2170941433 @default.
- W2149444911 cites W2171358858 @default.
- W2149444911 cites W2314016215 @default.
- W2149444911 cites W2316240194 @default.
- W2149444911 cites W2332059088 @default.
- W2149444911 cites W2408274948 @default.
- W2149444911 cites W2414474806 @default.
- W2149444911 cites W2419141963 @default.
- W2149444911 cites W1566044526 @default.
- W2149444911 cites W2409838860 @default.
- W2149444911 cites W3044574244 @default.
- W2149444911 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11106250" @default.
- W2149444911 hasPublicationYear "2000" @default.
- W2149444911 type Work @default.
- W2149444911 sameAs 2149444911 @default.
- W2149444911 citedByCount "27" @default.
- W2149444911 countsByYear W21494449112012 @default.
- W2149444911 countsByYear W21494449112015 @default.
- W2149444911 countsByYear W21494449112017 @default.
- W2149444911 countsByYear W21494449112018 @default.
- W2149444911 countsByYear W21494449112019 @default.
- W2149444911 crossrefType "journal-article" @default.
- W2149444911 hasAuthorship W2149444911A5009816072 @default.
- W2149444911 hasAuthorship W2149444911A5022987623 @default.
- W2149444911 hasAuthorship W2149444911A5026244234 @default.
- W2149444911 hasAuthorship W2149444911A5047657528 @default.
- W2149444911 hasAuthorship W2149444911A5050724475 @default.
- W2149444911 hasAuthorship W2149444911A5052212062 @default.
- W2149444911 hasAuthorship W2149444911A5080794406 @default.
- W2149444911 hasConcept C104317684 @default.
- W2149444911 hasConcept C111599444 @default.
- W2149444911 hasConcept C121608353 @default.
- W2149444911 hasConcept C135983454 @default.
- W2149444911 hasConcept C150194340 @default.
- W2149444911 hasConcept C153911025 @default.
- W2149444911 hasConcept C161733203 @default.
- W2149444911 hasConcept C2780427987 @default.
- W2149444911 hasConcept C32470452 @default.
- W2149444911 hasConcept C40767141 @default.
- W2149444911 hasConcept C502942594 @default.
- W2149444911 hasConcept C54009773 @default.
- W2149444911 hasConcept C54355233 @default.
- W2149444911 hasConcept C55493867 @default.
- W2149444911 hasConcept C81885089 @default.
- W2149444911 hasConcept C86803240 @default.
- W2149444911 hasConceptScore W2149444911C104317684 @default.