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• W2149452712 abstract "ABSTRACT Anthrax toxin consists of protective antigen (PA) and two toxic components, lethal factor (LF) and edema factor (EF). PA binds to mammalian cellular receptors and delivers the toxic components to the cytoplasm. PA is the primary antigenic component of the current anthrax vaccine. Immunity is due to the generation of antibodies that prevent the PA-mediated internalization of LF and EF. In this study, we characterized sera obtained from vaccinated military personnel. Anthrax vaccine is administered in a series of six injections at 0, 2, and 4 weeks and 6, 12, and 18 months, followed by annual boosters. The vaccination histories of the subjects were highly varied; many subjects had not completed the entire series, and several had not received annual boosters. We developed a simple colorimetric assay using alamarBlue dye to assess the antibody-mediated neutralization of LF-mediated toxicity to the J774A.1 murine macrophage cell line. Recently vaccinated individuals had high antibody levels and neutralizing activity. One individual who had not been boosted for 5 years had low immunoglobulin G antibody levels but a detectable neutralization activity, suggesting that this individual produced low levels of very active antibodies." @default.
• W2149452712 created "2016-06-24" @default.
• W2149452712 creator A5000685873 @default.
• W2149452712 creator A5006592308 @default.
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• W2149452712 date "2006-02-01" @default.
• W2149452712 modified "2023-09-23" @default.
• W2149452712 title "Neutralizing Antibodies and Persistence of Immunity following Anthrax Vaccination" @default.
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• W2149452712 doi "https://doi.org/10.1128/cvi.13.2.208-213.2006" @default.
• W2149452712 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1391949" @default.
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• W2149452712 hasPublicationYear "2006" @default.
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