FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149470189> ?p ?o ?g. }

• W2149470189 endingPage "1609" @default.
• W2149470189 startingPage "1604" @default.
• W2149470189 abstract "ABSTRACT In recombinant, xylose-fermenting Saccharomyces cerevisiae , about 30% of the consumed xylose is converted to xylitol. Xylitol production results from a cofactor imbalance, since xylose reductase uses both NADPH and NADH, while xylitol dehydrogenase uses only NAD + . In this study we increased the ethanol yield and decreased the xylitol yield by lowering the flux through the NADPH-producing pentose phosphate pathway. The pentose phosphate pathway was blocked either by disruption of the GND1 gene, one of the isogenes of 6-phosphogluconate dehydrogenase, or by disruption of the ZWF1 gene, which encodes glucose 6-phosphate dehydrogenase. Decreasing the phosphoglucose isomerase activity by 90% also lowered the pentose phosphate pathway flux. These modifications all resulted in lower xylitol yield and higher ethanol yield than in the control strains. TMB3255, carrying a disruption of ZWF1, gave the highest ethanol yield (0.41 g g −1 ) and the lowest xylitol yield (0.05 g g −1 ) reported for a xylose-fermenting recombinant S. cerevisiae strain, but also an 84% lower xylose consumption rate. The low xylose fermentation rate is probably due to limited NADPH-mediated xylose reduction. Metabolic flux modeling of TMB3255 confirmed that the NADPH-producing pentose phosphate pathway was blocked and that xylose reduction was mediated only by NADH, leading to a lower rate of xylose consumption. These results indicate that xylitol production is strongly connected to the flux through the oxidative part of the pentose phosphate pathway." @default.
• W2149470189 created "2016-06-24" @default.
• W2149470189 creator A5028558352 @default.
• W2149470189 creator A5036768624 @default.
• W2149470189 creator A5060977723 @default.
• W2149470189 creator A5081599626 @default.
• W2149470189 date "2002-04-01" @default.
• W2149470189 modified "2023-10-16" @default.
• W2149470189 title "Reduced Oxidative Pentose Phosphate Pathway Flux in Recombinant Xylose-Utilizing <i>Saccharomyces cerevisiae</i> Strains Improves the Ethanol Yield from Xylose" @default.
• W2149470189 cites W1044807534 @default.
• W2149470189 cites W1515252291 @default.
• W2149470189 cites W1520866295 @default.
• W2149470189 cites W1525974625 @default.
• W2149470189 cites W1526477746 @default.
• W2149470189 cites W1559650160 @default.
• W2149470189 cites W1850569493 @default.
• W2149470189 cites W1971554403 @default.
• W2149470189 cites W1990428308 @default.
• W2149470189 cites W1994715327 @default.
• W2149470189 cites W1994855175 @default.
• W2149470189 cites W1999851893 @default.
• W2149470189 cites W2012888408 @default.
• W2149470189 cites W2024450452 @default.
• W2149470189 cites W2026362025 @default.
• W2149470189 cites W2028622989 @default.
• W2149470189 cites W2033570693 @default.
• W2149470189 cites W2036541736 @default.
• W2149470189 cites W2042755961 @default.
• W2149470189 cites W2044747259 @default.
• W2149470189 cites W2055395487 @default.
• W2149470189 cites W2057282405 @default.
• W2149470189 cites W2061459002 @default.
• W2149470189 cites W2073270696 @default.
• W2149470189 cites W2074744858 @default.
• W2149470189 cites W2078297878 @default.
• W2149470189 cites W2080864219 @default.
• W2149470189 cites W2101442503 @default.
• W2149470189 cites W2110443726 @default.
• W2149470189 cites W2121356177 @default.
• W2149470189 cites W2150928193 @default.
• W2149470189 cites W2154102075 @default.
• W2149470189 cites W2158749368 @default.
• W2149470189 cites W2165762336 @default.
• W2149470189 cites W230886164 @default.
• W2149470189 cites W2320608314 @default.
• W2149470189 doi "https://doi.org/10.1128/aem.68.4.1604-1609.2002" @default.
• W2149470189 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/123863" @default.
• W2149470189 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11916674" @default.
• W2149470189 hasPublicationYear "2002" @default.
• W2149470189 type Work @default.
• W2149470189 sameAs 2149470189 @default.
• W2149470189 citedByCount "174" @default.
• W2149470189 countsByYear W21494701892012 @default.
• W2149470189 countsByYear W21494701892013 @default.
• W2149470189 countsByYear W21494701892014 @default.
• W2149470189 countsByYear W21494701892015 @default.
• W2149470189 countsByYear W21494701892016 @default.
• W2149470189 countsByYear W21494701892017 @default.
• W2149470189 countsByYear W21494701892018 @default.
• W2149470189 countsByYear W21494701892019 @default.
• W2149470189 countsByYear W21494701892020 @default.
• W2149470189 countsByYear W21494701892022 @default.
• W2149470189 countsByYear W21494701892023 @default.
• W2149470189 crossrefType "journal-article" @default.
• W2149470189 hasAuthorship W2149470189A5028558352 @default.
• W2149470189 hasAuthorship W2149470189A5036768624 @default.
• W2149470189 hasAuthorship W2149470189A5060977723 @default.
• W2149470189 hasAuthorship W2149470189A5081599626 @default.
• W2149470189 hasBestOaLocation W21494701892 @default.
• W2149470189 hasConcept C100544194 @default.
• W2149470189 hasConcept C185592680 @default.
• W2149470189 hasConcept C20251656 @default.
• W2149470189 hasConcept C2776031079 @default.
• W2149470189 hasConcept C2777809033 @default.
• W2149470189 hasConcept C2780261296 @default.
• W2149470189 hasConcept C2780294150 @default.
• W2149470189 hasConcept C2781135521 @default.
• W2149470189 hasConcept C3409486 @default.
• W2149470189 hasConcept C55493867 @default.
• W2149470189 hasConcept C62231903 @default.
• W2149470189 hasConceptScore W2149470189C100544194 @default.
• W2149470189 hasConceptScore W2149470189C185592680 @default.
• W2149470189 hasConceptScore W2149470189C20251656 @default.
• W2149470189 hasConceptScore W2149470189C2776031079 @default.
• W2149470189 hasConceptScore W2149470189C2777809033 @default.
• W2149470189 hasConceptScore W2149470189C2780261296 @default.
• W2149470189 hasConceptScore W2149470189C2780294150 @default.
• W2149470189 hasConceptScore W2149470189C2781135521 @default.
• W2149470189 hasConceptScore W2149470189C3409486 @default.
• W2149470189 hasConceptScore W2149470189C55493867 @default.
• W2149470189 hasConceptScore W2149470189C62231903 @default.
• W2149470189 hasIssue "4" @default.
• W2149470189 hasLocation W21494701891 @default.
• W2149470189 hasLocation W21494701892 @default.
• W2149470189 hasLocation W21494701893 @default.
• W2149470189 hasLocation W21494701894 @default.
• W2149470189 hasLocation W21494701895 @default.
• W2149470189 hasOpenAccess W2149470189 @default.

• next