Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149471888> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2149471888 endingPage "495a" @default.
- W2149471888 startingPage "495a" @default.
- W2149471888 abstract "Studies have shown that ion-channel function in immune cells such as macrophages can influence pathogen-induced immune signaling. Thus, ion channels are viewed by some researchers as potential therapeutic targets for developing novel strategies for regulating immune response on demand when standard anti-pathogen therapies such as antibiotics and vaccinations fall short. However, the direct contribution of ion-channel function to the complex and interconnected signaling pathways in immune response has proved elusive, largely due to the difficulty in tracking multiple signaling nodes in these pathways in real-time. Toward this end, we tracked the real-time inflammatory response to E. coli derived lipopolysaccharide (LPS) in a mouse macrophage-like cell-line (RAW 264.7) with electrophysiology to measure potassium channel currents and live imaging with fluorescent fusion reporters of crucial events involved in immune signaling. We developed two reporter constructs: 1) GFP fused to the NFκB transcription factor subunit RelA (GFP-RelA) to track early (<30 min) immune response, and 2) a TNFα promoter driving expression of mCherry with a terminal PEST sequence construct to track later (>2 hours) cytokine induction. In RAW264.7 cells, a 100 nM LPS challenge produces two waves of GFP-RelA translocation from the cytoplasm to the nucleus while gradually increasing the expression of mCherry (TNFα promoter activity). Continuous exposure of LPS-challenged cells to the BK- and Kv-channel blocker tetraethylammonium modifies the translocation dynamics of GFP-RelA and the induction of the TNFα promoter in a dose-dependent manner. Thus we provide evidence in support of a BK- and/or Kv-channel contribution to both early and later LPS induced inflammatory signaling." @default.
- W2149471888 created "2016-06-24" @default.
- W2149471888 creator A5022185559 @default.
- W2149471888 creator A5038596970 @default.
- W2149471888 creator A5040633142 @default.
- W2149471888 creator A5041382125 @default.
- W2149471888 creator A5048234086 @default.
- W2149471888 creator A5063028481 @default.
- W2149471888 creator A5071658484 @default.
- W2149471888 date "2010-01-01" @default.
- W2149471888 modified "2023-09-29" @default.
- W2149471888 title "Electrophysiology and Live Fluorescence Imaging to Monitor the Effects of Potassium Channel Blockade on Lipopolysaccharide-Induced Immune Signaling" @default.
- W2149471888 doi "https://doi.org/10.1016/j.bpj.2009.12.2697" @default.
- W2149471888 hasPublicationYear "2010" @default.
- W2149471888 type Work @default.
- W2149471888 sameAs 2149471888 @default.
- W2149471888 citedByCount "0" @default.
- W2149471888 crossrefType "journal-article" @default.
- W2149471888 hasAuthorship W2149471888A5022185559 @default.
- W2149471888 hasAuthorship W2149471888A5038596970 @default.
- W2149471888 hasAuthorship W2149471888A5040633142 @default.
- W2149471888 hasAuthorship W2149471888A5041382125 @default.
- W2149471888 hasAuthorship W2149471888A5048234086 @default.
- W2149471888 hasAuthorship W2149471888A5063028481 @default.
- W2149471888 hasAuthorship W2149471888A5071658484 @default.
- W2149471888 hasBestOaLocation W21494718881 @default.
- W2149471888 hasConcept C104317684 @default.
- W2149471888 hasConcept C1292079 @default.
- W2149471888 hasConcept C142613039 @default.
- W2149471888 hasConcept C17991360 @default.
- W2149471888 hasConcept C185592680 @default.
- W2149471888 hasConcept C203014093 @default.
- W2149471888 hasConcept C2778754761 @default.
- W2149471888 hasConcept C2779260011 @default.
- W2149471888 hasConcept C55493867 @default.
- W2149471888 hasConcept C62478195 @default.
- W2149471888 hasConcept C86339819 @default.
- W2149471888 hasConcept C86803240 @default.
- W2149471888 hasConcept C8891405 @default.
- W2149471888 hasConcept C95444343 @default.
- W2149471888 hasConceptScore W2149471888C104317684 @default.
- W2149471888 hasConceptScore W2149471888C1292079 @default.
- W2149471888 hasConceptScore W2149471888C142613039 @default.
- W2149471888 hasConceptScore W2149471888C17991360 @default.
- W2149471888 hasConceptScore W2149471888C185592680 @default.
- W2149471888 hasConceptScore W2149471888C203014093 @default.
- W2149471888 hasConceptScore W2149471888C2778754761 @default.
- W2149471888 hasConceptScore W2149471888C2779260011 @default.
- W2149471888 hasConceptScore W2149471888C55493867 @default.
- W2149471888 hasConceptScore W2149471888C62478195 @default.
- W2149471888 hasConceptScore W2149471888C86339819 @default.
- W2149471888 hasConceptScore W2149471888C86803240 @default.
- W2149471888 hasConceptScore W2149471888C8891405 @default.
- W2149471888 hasConceptScore W2149471888C95444343 @default.
- W2149471888 hasIssue "3" @default.
- W2149471888 hasLocation W21494718881 @default.
- W2149471888 hasOpenAccess W2149471888 @default.
- W2149471888 hasPrimaryLocation W21494718881 @default.
- W2149471888 hasRelatedWork W1964620595 @default.
- W2149471888 hasRelatedWork W2020335746 @default.
- W2149471888 hasRelatedWork W2023781164 @default.
- W2149471888 hasRelatedWork W2046142379 @default.
- W2149471888 hasRelatedWork W2097723155 @default.
- W2149471888 hasRelatedWork W2413000266 @default.
- W2149471888 hasRelatedWork W4231273468 @default.
- W2149471888 hasRelatedWork W4252199776 @default.
- W2149471888 hasRelatedWork W4295129685 @default.
- W2149471888 hasRelatedWork W2135689957 @default.
- W2149471888 hasVolume "98" @default.
- W2149471888 isParatext "false" @default.
- W2149471888 isRetracted "false" @default.
- W2149471888 magId "2149471888" @default.
- W2149471888 workType "article" @default.