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- W2149479927 abstract "Botulism is a paralytic disease caused by the botulinum neurotoxin produced by Clostridium botulinum. In the summer season in Korea, intensive outbreaks of avian botulism were reported in both poultry and wild birds, including five Korean native chicken farms (HanHyup NO.3), one pheasant (Phasianus colchicus karpowi) farm, and one community of spot-billed ducks (Anas poecilorhyncha). The affected domestic birds showed 24.5% to 58.3% mortality, with specific clinical signs including ataxia, limber neck, and diarrhea. To confirm the botulinum toxin, neutralization tests were performed on sera (four Korean native chicken farms and one pheasant farm) or culture supernatant (spot-billed ducks). Additionally, the contents of the cecum and liver from poultry presenting signs suggestive of botulism were inoculated to isolate the pathogen. The toxin genes were then detected by polymerase chain reaction (PCR). Through the neutralization tests, it was possible to diagnose the botulism and, except in the case of one Korean native chicken farm, to identify the type of pathogen. Using detection by PCR, except in two cases of the Korean native chicken farms, the botulinum toxin gene was found. Additionally, in four cases, it was possible to identify the C/D mosaic type using PCR. This paper reports the first occurrence of avian botulism in domestic birds and the first detection of botulism caused by this mosaic type in Korea.Reporte de caso – Presentación de botulismo aviar en Corea durante el período de junio a septiembre del año 2012.El botulismo es una enfermedad paralítica causada por la neurotoxina botulínica producida por Clostridium botulinum. Durante el verano, se registraron brotes de botulismo aviar intensivos tanto en aves comerciales y silvestres, incluyendo cinco granjas de pollos nativos (HanHyup NO.3) de Corea, una granja de faisanes (Phasianus colchicus karpowi) y una comunidad de ánades picopintos (Anas poecilorhyncha). Las aves domésticas afectadas mostraron de 24.5% a 58.3% de mortalidad, con signos clínicos específicos que incluyeron ataxia, flexibilidad de cuellos y diarrea. Para confirmar la presencia de la toxina botulínica, se realizaron pruebas de neutralización con suero (cuatro granjas de pollo nativo de Corea y una granja de faisanes) o con sobrenadante de cultivo (ánades picopintos). Además, los contenidos del ciego y el hígado de las aves comerciales que presentaban signos sugestivos de botulismo se inocularon para aislar el patógeno. Los genes de toxina fueron detectados mediante reacción en cadena de la polimerasa (PCR). Mediante pruebas de neutralización se pudo diagnosticar botulismo y con excepción de un caso de una granja de pollos nativos de Corea, se pudo identificar el tipo de patógeno. Mediante la detección por PCR se encontró el gene de la toxina botulínica, excepto en dos casos de las granjas de pollos nativos coreanos. Además, en cuatro casos, fue posible identificar la mezcla de los tipos C/D mediante PCR. Este trabajo presenta el primer reporte de botulismo aviar en aves domésticas y la primera detección de botulismo causado por la mezcla de estos tipos en Corea." @default.
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- W2149479927 date "2014-12-01" @default.
- W2149479927 modified "2023-09-26" @default.
- W2149479927 title "Occurrence of Avian Botulism in Korea During the Period from June to September 2012" @default.
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- W2149479927 doi "https://doi.org/10.1637/10793-020414-case" @default.
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