FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149502537> ?p ?o ?g. }

• W2149502537 endingPage "405" @default.
• W2149502537 startingPage "399" @default.
• W2149502537 abstract "Heterologous expression using baculovirus vectors has become a popular method for the production of catalytically active cytochrome P450s (CYPs). We have systematically optimized the multiplicity of infection (MOI) for a coinfection approach for the coexpression of CYP2A6 (viral vector designated v2A6) and NADPH-P450 oxidoreductase (OR; viral vector designated vOR) using Sf9 insect cells. A 3000-fold range of MOI was examined in stationary culture and stirred suspension culture. Surprisingly, our results indicate that the best CYP2A6 catalytic activity (850-1300 pmol/ min/mg total lysate protein as measured by coumarin 7-hydroxylase activity) was obtained only when using a low MOI of v2A6 (1.5-3 x 10(-2)) and a vOR of 10- to 20-fold less. This activity was approximately 7- to 11-fold higher than the best activity obtained when infecting cells with v2A6 alone. At this level of coinfection, the P450 content ranged from 180 to 250 pmol/mg total lysate protein, and the NADPH cytochrome c reductase activity ranged from 350 to 520 nmol/min/mg total lysate protein. Increasing the MOI of both viruses to 50-fold higher resulted in lower overall activity with the optimum (250 pmol/min/mg total lysate protein) being seen earlier postinfection (60 vs. 72 hr). Increasing the MOI of vOR to levels comparable with those of v2A6, decreased coumarin 7-hydroxylase activity 14-fold. These results suggest that the best CYP2A6 catalytic activity depends on properly posttranslationally modified proteins accumulating in a right ratio as a result of primary, secondary, and possibly tertiary infection of both viruses. These results also suggest that high OR expression results in degradation of P450." @default.
• W2149502537 created "2016-06-24" @default.
• W2149502537 creator A5023776289 @default.
• W2149502537 creator A5043742507 @default.
• W2149502537 creator A5056191318 @default.
• W2149502537 creator A5062110198 @default.
• W2149502537 creator A5069841884 @default.
• W2149502537 creator A5073932699 @default.
• W2149502537 creator A5088332392 @default.
• W2149502537 date "1997-04-01" @default.
• W2149502537 modified "2023-10-01" @default.
• W2149502537 title "Coexpression of cytochrome P4502A6 and human NADPH-P450 oxidoreductase in the baculovirus system." @default.
• W2149502537 cites W1018436991 @default.
• W2149502537 cites W1484177930 @default.
• W2149502537 cites W1488793801 @default.
• W2149502537 cites W1532212858 @default.
• W2149502537 cites W1570536697 @default.
• W2149502537 cites W1602419259 @default.
• W2149502537 cites W1639043875 @default.
• W2149502537 cites W1680499713 @default.
• W2149502537 cites W1786736016 @default.
• W2149502537 cites W1949792042 @default.
• W2149502537 cites W1974993420 @default.
• W2149502537 cites W1977076419 @default.
• W2149502537 cites W1993945284 @default.
• W2149502537 cites W1998707670 @default.
• W2149502537 cites W1998984889 @default.
• W2149502537 cites W2007972960 @default.
• W2149502537 cites W2011795894 @default.
• W2149502537 cites W2028690842 @default.
• W2149502537 cites W2045333955 @default.
• W2149502537 cites W2050398889 @default.
• W2149502537 cites W2051735125 @default.
• W2149502537 cites W2054972846 @default.
• W2149502537 cites W2060842763 @default.
• W2149502537 cites W2065449693 @default.
• W2149502537 cites W2067525361 @default.
• W2149502537 cites W2076657129 @default.
• W2149502537 cites W2079118845 @default.
• W2149502537 cites W2080871194 @default.
• W2149502537 cites W2082119404 @default.
• W2149502537 cites W2094932400 @default.
• W2149502537 cites W2096297778 @default.
• W2149502537 cites W2143481327 @default.
• W2149502537 cites W2148849061 @default.
• W2149502537 cites W2169167768 @default.
• W2149502537 cites W2296717859 @default.
• W2149502537 cites W2399984614 @default.
• W2149502537 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9107537" @default.
• W2149502537 hasPublicationYear "1997" @default.
• W2149502537 type Work @default.
• W2149502537 sameAs 2149502537 @default.
• W2149502537 citedByCount "15" @default.
• W2149502537 countsByYear W21495025372012 @default.
• W2149502537 countsByYear W21495025372017 @default.
• W2149502537 countsByYear W21495025372018 @default.
• W2149502537 countsByYear W21495025372022 @default.
• W2149502537 countsByYear W21495025372023 @default.
• W2149502537 crossrefType "journal-article" @default.
• W2149502537 hasAuthorship W2149502537A5023776289 @default.
• W2149502537 hasAuthorship W2149502537A5043742507 @default.
• W2149502537 hasAuthorship W2149502537A5056191318 @default.
• W2149502537 hasAuthorship W2149502537A5062110198 @default.
• W2149502537 hasAuthorship W2149502537A5069841884 @default.
• W2149502537 hasAuthorship W2149502537A5073932699 @default.
• W2149502537 hasAuthorship W2149502537A5088332392 @default.
• W2149502537 hasConcept C103878061 @default.
• W2149502537 hasConcept C104317684 @default.
• W2149502537 hasConcept C109650736 @default.
• W2149502537 hasConcept C134651460 @default.
• W2149502537 hasConcept C153911025 @default.
• W2149502537 hasConcept C181199279 @default.
• W2149502537 hasConcept C196013638 @default.
• W2149502537 hasConcept C198684833 @default.
• W2149502537 hasConcept C202751555 @default.
• W2149502537 hasConcept C203233990 @default.
• W2149502537 hasConcept C2777773057 @default.
• W2149502537 hasConcept C2779268744 @default.
• W2149502537 hasConcept C2780115692 @default.
• W2149502537 hasConcept C40767141 @default.
• W2149502537 hasConcept C526171541 @default.
• W2149502537 hasConcept C55493867 @default.
• W2149502537 hasConcept C57409179 @default.
• W2149502537 hasConcept C86803240 @default.
• W2149502537 hasConceptScore W2149502537C103878061 @default.
• W2149502537 hasConceptScore W2149502537C104317684 @default.
• W2149502537 hasConceptScore W2149502537C109650736 @default.
• W2149502537 hasConceptScore W2149502537C134651460 @default.
• W2149502537 hasConceptScore W2149502537C153911025 @default.
• W2149502537 hasConceptScore W2149502537C181199279 @default.
• W2149502537 hasConceptScore W2149502537C196013638 @default.
• W2149502537 hasConceptScore W2149502537C198684833 @default.
• W2149502537 hasConceptScore W2149502537C202751555 @default.
• W2149502537 hasConceptScore W2149502537C203233990 @default.
• W2149502537 hasConceptScore W2149502537C2777773057 @default.
• W2149502537 hasConceptScore W2149502537C2779268744 @default.
• W2149502537 hasConceptScore W2149502537C2780115692 @default.
• W2149502537 hasConceptScore W2149502537C40767141 @default.

• next