FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149544286> ?p ?o ?g. }

• W2149544286 endingPage "64" @default.
• W2149544286 startingPage "5156" @default.
• W2149544286 abstract "Synthetic oligodeoxyribonucleotides (ODNs) designed to selectively inhibit the transcription or translation of specific genes are being used to modulate the activity of the targeted gene. Because multiple copies of mRNA can be transcribed from one actively expressed gene, ODNs that target double-stranded DNA and form triple helices upon binding with the gene itself have an advantage over ODNs that target the gene product (mRNA) in an antisense fashion. For the present studies, we designed four different triple helix-forming phosphodiester ODNs (TFOs) targeted to the tumor necrosis factor (TNF) gene and examined their effect on production of TNF and on cellular growth of tumors in which TNF acts as an autocrine growth factor. The ODNs J-109-50 and J-108-57 were designed to interact with polypurine oligonucleotides corresponding to the binding sites for nuclear factors kB (-237 to -208) and Sp1 (-58 to -33), respectively; J111-51 was designed to interact with a polypurine oligonucleotide in the third intron (+1429 to +1456) of the TNF gene. To enhance the cellular penetration and prevent degradation by cellular nucleases, the TFOs were modified at their 3' ends by either a cholesterol side chain or a propanolamine blocking group. Treatment of the human promonocytic cell line THP-1 with TNF-TFOs at a nontoxic concentration (2 microM) reduced the production of TNF. All of the TNF-TFOs tested were effective, and control-irrelevant TFOs were ineffective in inhibiting TNF production. The activity of the most efficacious TNF-TFOs also correlated with a decrease in TNF mRNA as observed by using reverse transcriptase PCR assays. In several tumors in which TNF acts as an autocrine growth factor, we examined the antiproliferative activity of J111-51. We found that in the human glioblastoma tumor cell line U-251, TNF-induced growth was blocked by J111-51 in a dose-dependent manner. Thus, overall results demonstrate that oligonucleotides directed to the specific regions of TNF can be designed, which may have a potential in cancer therapy." @default.
• W2149544286 created "2016-06-24" @default.
• W2149544286 creator A5010282324 @default.
• W2149544286 creator A5027059891 @default.
• W2149544286 creator A5048675624 @default.
• W2149544286 creator A5090879920 @default.
• W2149544286 date "1996-11-15" @default.
• W2149544286 modified "2023-09-26" @default.
• W2149544286 title "Triple helix-forming oligodeoxyribonucleotides targeted to the human tumor necrosis factor (TNF) gene inhibit TNF production and block the TNF-dependent growth of human glioblastoma tumor cells." @default.
• W2149544286 cites W1480548164 @default.
• W2149544286 cites W1499463091 @default.
• W2149544286 cites W1503094954 @default.
• W2149544286 cites W1534181850 @default.
• W2149544286 cites W1552647544 @default.
• W2149544286 cites W1610133400 @default.
• W2149544286 cites W1672163784 @default.
• W2149544286 cites W1763490035 @default.
• W2149544286 cites W1765237838 @default.
• W2149544286 cites W1964858690 @default.
• W2149544286 cites W1974203712 @default.
• W2149544286 cites W1984545988 @default.
• W2149544286 cites W1989783048 @default.
• W2149544286 cites W1995762900 @default.
• W2149544286 cites W2000669063 @default.
• W2149544286 cites W2032069911 @default.
• W2149544286 cites W2034908174 @default.
• W2149544286 cites W2038271482 @default.
• W2149544286 cites W2048234476 @default.
• W2149544286 cites W2048325240 @default.
• W2149544286 cites W2050487488 @default.
• W2149544286 cites W2055750024 @default.
• W2149544286 cites W2055772243 @default.
• W2149544286 cites W2060546037 @default.
• W2149544286 cites W2061419481 @default.
• W2149544286 cites W2077281274 @default.
• W2149544286 cites W2083116993 @default.
• W2149544286 cites W2083726090 @default.
• W2149544286 cites W2091627659 @default.
• W2149544286 cites W2096158027 @default.
• W2149544286 cites W2116656764 @default.
• W2149544286 cites W2120178047 @default.
• W2149544286 cites W2128456041 @default.
• W2149544286 cites W2134812217 @default.
• W2149544286 cites W2321116935 @default.
• W2149544286 cites W2404612463 @default.
• W2149544286 cites W2407933525 @default.
• W2149544286 cites W3145981933 @default.
• W2149544286 cites W580064426 @default.
• W2149544286 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8912851" @default.
• W2149544286 hasPublicationYear "1996" @default.
• W2149544286 type Work @default.
• W2149544286 sameAs 2149544286 @default.
• W2149544286 citedByCount "26" @default.
• W2149544286 countsByYear W21495442862012 @default.
• W2149544286 countsByYear W21495442862013 @default.
• W2149544286 countsByYear W21495442862014 @default.
• W2149544286 countsByYear W21495442862015 @default.
• W2149544286 countsByYear W21495442862016 @default.
• W2149544286 crossrefType "journal-article" @default.
• W2149544286 hasAuthorship W2149544286A5010282324 @default.
• W2149544286 hasAuthorship W2149544286A5027059891 @default.
• W2149544286 hasAuthorship W2149544286A5048675624 @default.
• W2149544286 hasAuthorship W2149544286A5090879920 @default.
• W2149544286 hasConcept C104317684 @default.
• W2149544286 hasConcept C105580179 @default.
• W2149544286 hasConcept C129312508 @default.
• W2149544286 hasConcept C150194340 @default.
• W2149544286 hasConcept C153911025 @default.
• W2149544286 hasConcept C17991360 @default.
• W2149544286 hasConcept C203014093 @default.
• W2149544286 hasConcept C502942594 @default.
• W2149544286 hasConcept C55493867 @default.
• W2149544286 hasConcept C86803240 @default.
• W2149544286 hasConceptScore W2149544286C104317684 @default.
• W2149544286 hasConceptScore W2149544286C105580179 @default.
• W2149544286 hasConceptScore W2149544286C129312508 @default.
• W2149544286 hasConceptScore W2149544286C150194340 @default.
• W2149544286 hasConceptScore W2149544286C153911025 @default.
• W2149544286 hasConceptScore W2149544286C17991360 @default.
• W2149544286 hasConceptScore W2149544286C203014093 @default.
• W2149544286 hasConceptScore W2149544286C502942594 @default.
• W2149544286 hasConceptScore W2149544286C55493867 @default.
• W2149544286 hasConceptScore W2149544286C86803240 @default.
• W2149544286 hasIssue "22" @default.
• W2149544286 hasLocation W21495442861 @default.
• W2149544286 hasOpenAccess W2149544286 @default.
• W2149544286 hasPrimaryLocation W21495442861 @default.
• W2149544286 hasRelatedWork W1956097919 @default.
• W2149544286 hasRelatedWork W1973757137 @default.
• W2149544286 hasRelatedWork W2026554087 @default.
• W2149544286 hasRelatedWork W2043208644 @default.
• W2149544286 hasRelatedWork W2064375428 @default.
• W2149544286 hasRelatedWork W2327308558 @default.
• W2149544286 hasRelatedWork W2372350704 @default.
• W2149544286 hasRelatedWork W2401184364 @default.
• W2149544286 hasRelatedWork W2405769460 @default.
• W2149544286 hasRelatedWork W4246242683 @default.
• W2149544286 hasVolume "56" @default.

• next