FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149571723> ?p ?o ?g. }

• W2149571723 endingPage "5162" @default.
• W2149571723 startingPage "5158" @default.
• W2149571723 abstract "We report here on the isolation and characterization of a serotonin (5HT) transporter from Drosophila melanogaster. A 3.1-kb complementary DNA clone (dSERT) was found to encode a protein of 622 amino acid residues with a predicted molecular mass of approximately 69 kDa and a putative transmembrane topology characteristic of cloned members of the mammalian Na+/Cl- neurotransmitter cotransporter gene family. dSERT displays highest overall amino acid sequence identity with the mammalian 5HT (51%), norepinephrine (47%), and dopamine (47%) transporters and shares with all transporters 104 absolutely conserved amino acid residues. Upon transient expression in HeLa cells, dSERT exhibited saturable, high-affinity, and sodium-dependent [3H]5HT uptake with estimated Km and Vmax values of approximately 500 nM and 5.2 x 10(-18) mol per cell per min, respectively. In marked contrast to the human SERT (hSERT), 5HT-mediated transport by dSERT was not absolutely dependent on extracellular Cl-, while the sodium-dependent uptake of 5HT was facilitated by increased extracellular Cl- concentrations. dSERT displays a pharmacological profile and rank order of potency consistent with, but not identical to, mammalian 5HT transporters. Comparison of the affinities of various compounds for the inhibition of 5HT transport by both dSERT and hSERT revealed that antidepressants were 3- to 300-fold less potent on dSERT than on hSERT, while mazindol displayed approximately 30-fold greater potency for dSERT. Both cocaine and RTI-55 inhibited 5HT uptake by dSERT with estimated inhibition constants of approximately 500 nM, while high concentrations (> 10 microM) of dopamine, norepinephrine, octopamine, tyramine, and histamine failed to inhibit transport. In situ hybridization reveals the selective expression of dSERT mRNA to specific cell bodies in the ventral ganglion of the embryonic and larval Drosophila nervous system with a distribution pattern virtually identical to that of 5HT-containing neurons. The dSERT gene was mapped to position 60C on chromosome 2. The availability of the gene encoding the unique ion dependence and pharmacological characteristics of dSERT may allow for identification of those amino acid residues and structural motifs that confer the pharmacologic specificity and genetic regulation of the 5HT transport process." @default.
• W2149571723 created "2016-06-24" @default.
• W2149571723 creator A5008230738 @default.
• W2149571723 creator A5014335052 @default.
• W2149571723 creator A5017325982 @default.
• W2149571723 creator A5022425643 @default.
• W2149571723 creator A5050853442 @default.
• W2149571723 creator A5054347925 @default.
• W2149571723 creator A5084826064 @default.
• W2149571723 creator A5089616153 @default.
• W2149571723 date "1994-05-24" @default.
• W2149571723 modified "2023-10-16" @default.
• W2149571723 title "Cloning, expression, and localization of a chloride-facilitated, cocaine-sensitive serotonin transporter from Drosophila melanogaster." @default.
• W2149571723 cites W14143485 @default.
• W2149571723 cites W1499693439 @default.
• W2149571723 cites W1562036320 @default.
• W2149571723 cites W1562634795 @default.
• W2149571723 cites W1889871926 @default.
• W2149571723 cites W1938622267 @default.
• W2149571723 cites W1980253852 @default.
• W2149571723 cites W1980263219 @default.
• W2149571723 cites W1991442236 @default.
• W2149571723 cites W1997460720 @default.
• W2149571723 cites W1997634040 @default.
• W2149571723 cites W2000904449 @default.
• W2149571723 cites W2005393972 @default.
• W2149571723 cites W2005411001 @default.
• W2149571723 cites W2008215698 @default.
• W2149571723 cites W2018580557 @default.
• W2149571723 cites W2026154759 @default.
• W2149571723 cites W2028394518 @default.
• W2149571723 cites W2032226112 @default.
• W2149571723 cites W2033067631 @default.
• W2149571723 cites W2033414490 @default.
• W2149571723 cites W2036511379 @default.
• W2149571723 cites W2037411216 @default.
• W2149571723 cites W2042096080 @default.
• W2149571723 cites W2051918534 @default.
• W2149571723 cites W2056960549 @default.
• W2149571723 cites W2057086528 @default.
• W2149571723 cites W2063752616 @default.
• W2149571723 cites W2067805105 @default.
• W2149571723 cites W2074631079 @default.
• W2149571723 cites W2075290855 @default.
• W2149571723 cites W2078866571 @default.
• W2149571723 cites W2079204641 @default.
• W2149571723 cites W2079507733 @default.
• W2149571723 cites W2104947539 @default.
• W2149571723 cites W2119851366 @default.
• W2149571723 cites W2124549431 @default.
• W2149571723 cites W2145533695 @default.
• W2149571723 cites W2154128645 @default.
• W2149571723 cites W2174425677 @default.
• W2149571723 cites W2402489441 @default.
• W2149571723 cites W2405501714 @default.
• W2149571723 cites W2418259786 @default.
• W2149571723 cites W75664332 @default.
• W2149571723 doi "https://doi.org/10.1073/pnas.91.11.5158" @default.
• W2149571723 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/43951" @default.
• W2149571723 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8197200" @default.
• W2149571723 hasPublicationYear "1994" @default.
• W2149571723 type Work @default.
• W2149571723 sameAs 2149571723 @default.
• W2149571723 citedByCount "148" @default.
• W2149571723 countsByYear W21495717232012 @default.
• W2149571723 countsByYear W21495717232013 @default.
• W2149571723 countsByYear W21495717232014 @default.
• W2149571723 countsByYear W21495717232015 @default.
• W2149571723 countsByYear W21495717232016 @default.
• W2149571723 countsByYear W21495717232017 @default.
• W2149571723 countsByYear W21495717232018 @default.
• W2149571723 countsByYear W21495717232019 @default.
• W2149571723 countsByYear W21495717232020 @default.
• W2149571723 countsByYear W21495717232021 @default.
• W2149571723 countsByYear W21495717232022 @default.
• W2149571723 countsByYear W21495717232023 @default.
• W2149571723 crossrefType "journal-article" @default.
• W2149571723 hasAuthorship W2149571723A5008230738 @default.
• W2149571723 hasAuthorship W2149571723A5014335052 @default.
• W2149571723 hasAuthorship W2149571723A5017325982 @default.
• W2149571723 hasAuthorship W2149571723A5022425643 @default.
• W2149571723 hasAuthorship W2149571723A5050853442 @default.
• W2149571723 hasAuthorship W2149571723A5054347925 @default.
• W2149571723 hasAuthorship W2149571723A5084826064 @default.
• W2149571723 hasAuthorship W2149571723A5089616153 @default.
• W2149571723 hasBestOaLocation W21495717232 @default.
• W2149571723 hasConcept C104317684 @default.
• W2149571723 hasConcept C149011108 @default.
• W2149571723 hasConcept C170493617 @default.
• W2149571723 hasConcept C176233148 @default.
• W2149571723 hasConcept C2775864247 @default.
• W2149571723 hasConcept C2776755682 @default.
• W2149571723 hasConcept C2778897192 @default.
• W2149571723 hasConcept C2909816965 @default.
• W2149571723 hasConcept C515207424 @default.
• W2149571723 hasConcept C55493867 @default.
• W2149571723 hasConcept C86803240 @default.
• W2149571723 hasConceptScore W2149571723C104317684 @default.

• next