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- W2149605836 abstract "Calcium (Ca 2+ ) influx is required for the sustained secretion of insulin and is accompanied by a large rate of energy usage. We hypothesize that the energy usage reflects a process [Ca 2+ /metabolic coupling process (CMCP)] that couples Ca 2+ to insulin secretion by pancreatic islets. The aim of the study was to test this hypothesis by testing the effect of inhibiting candidate Ca 2+ -sensitive proteins proposed to play a critical role in the CMCP. The effects of the inhibitors on oxygen consumption rate (OCR), a reflection of ATP usage, and insulin secretion rate (ISR) were compared with those seen when L-type Ca 2+ channels were blocked with nimodipine. We reasoned that if a downstream Ca 2+ -regulated site was responsible for the OCR associated with the CMCP, then its inhibition should mimic the effect of nimodipine. Consistent with previous findings, nimodipine decreased glucose-stimulated OCR by 36% and cytosolic Ca 2+ by 46% and completely suppressed ISR in rat pancreatic islets. Inhibitors of three calmodulin-sensitive proteins (myosin light-chain kinase, calcineurin, and Ca 2+ /calmodulin-dependent protein kinase II) did not meet the criteria. In contrast, KN-62 severed the connection between Ca 2+ influx, OCR, and ISR without interfering with Ca 2+ influx. In the presence of nimodipine or KN-62, potentiators of ISR, acetylcholine, GLP-1, and arginine had little effect on insulin secretion, suggesting that the CMCP is also essential for the amplification of ISR. In conclusion, a KN-62-sensitive process directly mediates the effects of Ca 2+ influx via L-type Ca 2+ channels on OCR and ISR, supporting the essential role of the CMCP in mediating ISR." @default.
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- W2149605836 date "2009-09-01" @default.
- W2149605836 modified "2023-09-24" @default.
- W2149605836 title "A highly energetic process couples calcium influx through L-type calcium channels to insulin secretion in pancreatic β-cells" @default.
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- W2149605836 doi "https://doi.org/10.1152/ajpendo.00282.2009" @default.
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