Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149647571> ?p ?o ?g. }
- W2149647571 endingPage "270" @default.
- W2149647571 startingPage "260" @default.
- W2149647571 abstract "Chondroitin AC lyase (chondroitinase EC 4.2.2.5), an eliminase from Flavobacterium heparinum, cleaves chondroitin sulfate glycosaminoglycans (GAGs) at 1,4 glycosidic linkages between N-acetylgalactosamine and glucuronic acid residues. Cleavage occurs through beta-elimination in a random endolytic action pattern. Crystal structures of chondroitin AC lyase (wild type) complexed with oligosaccharides reveal a binding site within a narrow and shallow protein channel, suggesting several amino acids as candidates for the active site residues. Site-specific mutagenesis studies on residues within the active-site tunnel revealed that only the Arg to Ala 292 mutation (R292A) retained activity. Furthermore, structural data suggested that R292 was primarily involved in recognition of N-acetyl or O-sulfo moieties of galactosamine residues and did not directly participate in catalysis. The current study demonstrates that the R292A mutation affords approximately 10-fold higher K(m) values but no significant change in V(max), consistent with hypothesis that R292 is involved in binding the O-sulfo moiety of the saccharide residues. Change in chondroitin sulfate viscosity, as a function of its enzymatic cleavage, affords a shallower concave curve for the R292A mutant, suggesting its action pattern is neither purely random endolytic nor purely random exolytic. Product studies using gel electrophoresis confirm the altered action pattern of this mutant. Thus, these data suggest that the R292A mutation effectively reduces binding affinity, making it possible for the oligosaccharide chain, still bound after initial endolytic cleavage, to slide through the tunnel to the catalytic site for subsequent, processive, step-wise, exolytic cleavage." @default.
- W2149647571 created "2016-06-24" @default.
- W2149647571 creator A5005759921 @default.
- W2149647571 creator A5010480312 @default.
- W2149647571 creator A5027287092 @default.
- W2149647571 creator A5029668234 @default.
- W2149647571 creator A5056210370 @default.
- W2149647571 creator A5075950859 @default.
- W2149647571 date "2002-06-01" @default.
- W2149647571 modified "2023-09-23" @default.
- W2149647571 title "Role of arginine 292 in the catalytic activity of chondroitin AC lyase from Flavobacterium heparinum" @default.
- W2149647571 cites W1490336029 @default.
- W2149647571 cites W1543709948 @default.
- W2149647571 cites W1553601962 @default.
- W2149647571 cites W1576040672 @default.
- W2149647571 cites W1658991581 @default.
- W2149647571 cites W1787704759 @default.
- W2149647571 cites W1844848865 @default.
- W2149647571 cites W1908231126 @default.
- W2149647571 cites W1935712031 @default.
- W2149647571 cites W1964279075 @default.
- W2149647571 cites W1965649683 @default.
- W2149647571 cites W1967222812 @default.
- W2149647571 cites W1967227961 @default.
- W2149647571 cites W1969148830 @default.
- W2149647571 cites W1975753413 @default.
- W2149647571 cites W1984758622 @default.
- W2149647571 cites W1987273269 @default.
- W2149647571 cites W1993119187 @default.
- W2149647571 cites W1997096247 @default.
- W2149647571 cites W2005976832 @default.
- W2149647571 cites W2008160877 @default.
- W2149647571 cites W2011488277 @default.
- W2149647571 cites W2017977583 @default.
- W2149647571 cites W2026055816 @default.
- W2149647571 cites W2029336097 @default.
- W2149647571 cites W2035970994 @default.
- W2149647571 cites W2036548032 @default.
- W2149647571 cites W2052769066 @default.
- W2149647571 cites W2055595832 @default.
- W2149647571 cites W2080133860 @default.
- W2149647571 cites W2091502955 @default.
- W2149647571 cites W2110196399 @default.
- W2149647571 cites W2123199112 @default.
- W2149647571 cites W2127950208 @default.
- W2149647571 cites W2147645628 @default.
- W2149647571 cites W2147732011 @default.
- W2149647571 cites W2318235807 @default.
- W2149647571 cites W3189759260 @default.
- W2149647571 cites W4236450166 @default.
- W2149647571 cites W4243003257 @default.
- W2149647571 doi "https://doi.org/10.1016/s0167-4838(02)00304-7" @default.
- W2149647571 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12044904" @default.
- W2149647571 hasPublicationYear "2002" @default.
- W2149647571 type Work @default.
- W2149647571 sameAs 2149647571 @default.
- W2149647571 citedByCount "18" @default.
- W2149647571 countsByYear W21496475712013 @default.
- W2149647571 countsByYear W21496475712016 @default.
- W2149647571 countsByYear W21496475712019 @default.
- W2149647571 countsByYear W21496475712022 @default.
- W2149647571 crossrefType "journal-article" @default.
- W2149647571 hasAuthorship W2149647571A5005759921 @default.
- W2149647571 hasAuthorship W2149647571A5010480312 @default.
- W2149647571 hasAuthorship W2149647571A5027287092 @default.
- W2149647571 hasAuthorship W2149647571A5029668234 @default.
- W2149647571 hasAuthorship W2149647571A5056210370 @default.
- W2149647571 hasAuthorship W2149647571A5075950859 @default.
- W2149647571 hasConcept C100817775 @default.
- W2149647571 hasConcept C104317684 @default.
- W2149647571 hasConcept C107824862 @default.
- W2149647571 hasConcept C143065580 @default.
- W2149647571 hasConcept C150487720 @default.
- W2149647571 hasConcept C151730666 @default.
- W2149647571 hasConcept C153074725 @default.
- W2149647571 hasConcept C175156509 @default.
- W2149647571 hasConcept C181199279 @default.
- W2149647571 hasConcept C185592680 @default.
- W2149647571 hasConcept C2776648616 @default.
- W2149647571 hasConcept C2776735749 @default.
- W2149647571 hasConcept C2777269803 @default.
- W2149647571 hasConcept C2777726330 @default.
- W2149647571 hasConcept C2779553658 @default.
- W2149647571 hasConcept C41183919 @default.
- W2149647571 hasConcept C43369102 @default.
- W2149647571 hasConcept C55493867 @default.
- W2149647571 hasConcept C71240020 @default.
- W2149647571 hasConcept C86803240 @default.
- W2149647571 hasConceptScore W2149647571C100817775 @default.
- W2149647571 hasConceptScore W2149647571C104317684 @default.
- W2149647571 hasConceptScore W2149647571C107824862 @default.
- W2149647571 hasConceptScore W2149647571C143065580 @default.
- W2149647571 hasConceptScore W2149647571C150487720 @default.
- W2149647571 hasConceptScore W2149647571C151730666 @default.
- W2149647571 hasConceptScore W2149647571C153074725 @default.
- W2149647571 hasConceptScore W2149647571C175156509 @default.
- W2149647571 hasConceptScore W2149647571C181199279 @default.
- W2149647571 hasConceptScore W2149647571C185592680 @default.