Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149656347> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2149656347 endingPage "1260" @default.
- W2149656347 startingPage "1255" @default.
- W2149656347 abstract "Abstract Background: A reliable indicator of cholestasis is the presence of abnormal concentrations of bilirubin mono- and diglucuronide [conjugated bilirubin (CB)] in blood. A routine assay of CB is available only to those who possess a certain type of clinical analyzer. We describe a two-point manual method for CB that could be adapted as a rate assay to automated clinical analyzers. Methods: The measurement of CB is based on its oxidation to biliverdin by bilirubin oxidase. The resulting decrease in absorbance at 460 nm is proportional to the CB concentration. The assay is calibrated with solutions of ditaurobilirubin in human serum. Results: Under the conditions of the assay (0.1 mol/L glycine buffer, pH 10.0; reaction time, 2 min), only 5% of unconjugated bilirubin is oxidized and δ-bilirubin is not oxidized at all. Results obtained with the bilirubin oxidase method agreed well with those obtained by HPLC. The long-term CVs at CB concentrations of 6 and 63.4 mg/L were 20% and 2.6%, respectively. The reference values, established by analyzing 51 plasma specimens from healthy adults, were 0.0–1.2 mg/L, with a mean value of 0.2 mg/L. Conclusions: The proposed method for CB has good analytical specificity and obviates the requirement for HPLC or a dry chemistry analyzer. The measurement of CB in blood is superior to the measurement of direct bilirubin because an abnormal concentration of direct bilirubin does not necessarily indicate the presence of cholestasis." @default.
- W2149656347 created "2016-06-24" @default.
- W2149656347 creator A5005949222 @default.
- W2149656347 creator A5010294963 @default.
- W2149656347 creator A5024635066 @default.
- W2149656347 creator A5061120504 @default.
- W2149656347 creator A5081740321 @default.
- W2149656347 date "1999-08-01" @default.
- W2149656347 modified "2023-09-26" @default.
- W2149656347 title "Determination of the Sum of Bilirubin Sugar Conjugates in Plasma by Bilirubin Oxidase" @default.
- W2149656347 cites W1906912621 @default.
- W2149656347 cites W1931321902 @default.
- W2149656347 cites W1940321413 @default.
- W2149656347 cites W2005491475 @default.
- W2149656347 cites W2159591197 @default.
- W2149656347 cites W4300424288 @default.
- W2149656347 doi "https://doi.org/10.1093/clinchem/45.8.1255" @default.
- W2149656347 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10430792" @default.
- W2149656347 hasPublicationYear "1999" @default.
- W2149656347 type Work @default.
- W2149656347 sameAs 2149656347 @default.
- W2149656347 citedByCount "24" @default.
- W2149656347 countsByYear W21496563472012 @default.
- W2149656347 countsByYear W21496563472014 @default.
- W2149656347 countsByYear W21496563472016 @default.
- W2149656347 countsByYear W21496563472018 @default.
- W2149656347 countsByYear W21496563472019 @default.
- W2149656347 countsByYear W21496563472020 @default.
- W2149656347 countsByYear W21496563472021 @default.
- W2149656347 crossrefType "journal-article" @default.
- W2149656347 hasAuthorship W2149656347A5005949222 @default.
- W2149656347 hasAuthorship W2149656347A5010294963 @default.
- W2149656347 hasAuthorship W2149656347A5024635066 @default.
- W2149656347 hasAuthorship W2149656347A5061120504 @default.
- W2149656347 hasAuthorship W2149656347A5081740321 @default.
- W2149656347 hasBestOaLocation W21496563471 @default.
- W2149656347 hasConcept C126322002 @default.
- W2149656347 hasConcept C160160445 @default.
- W2149656347 hasConcept C181199279 @default.
- W2149656347 hasConcept C185592680 @default.
- W2149656347 hasConcept C2777513400 @default.
- W2149656347 hasConcept C2777915509 @default.
- W2149656347 hasConcept C2909331925 @default.
- W2149656347 hasConcept C43617362 @default.
- W2149656347 hasConcept C55493867 @default.
- W2149656347 hasConcept C71924100 @default.
- W2149656347 hasConcept C98015330 @default.
- W2149656347 hasConceptScore W2149656347C126322002 @default.
- W2149656347 hasConceptScore W2149656347C160160445 @default.
- W2149656347 hasConceptScore W2149656347C181199279 @default.
- W2149656347 hasConceptScore W2149656347C185592680 @default.
- W2149656347 hasConceptScore W2149656347C2777513400 @default.
- W2149656347 hasConceptScore W2149656347C2777915509 @default.
- W2149656347 hasConceptScore W2149656347C2909331925 @default.
- W2149656347 hasConceptScore W2149656347C43617362 @default.
- W2149656347 hasConceptScore W2149656347C55493867 @default.
- W2149656347 hasConceptScore W2149656347C71924100 @default.
- W2149656347 hasConceptScore W2149656347C98015330 @default.
- W2149656347 hasIssue "8" @default.
- W2149656347 hasLocation W21496563471 @default.
- W2149656347 hasLocation W21496563472 @default.
- W2149656347 hasLocation W21496563473 @default.
- W2149656347 hasOpenAccess W2149656347 @default.
- W2149656347 hasPrimaryLocation W21496563471 @default.
- W2149656347 hasRelatedWork W1919420982 @default.
- W2149656347 hasRelatedWork W1946026855 @default.
- W2149656347 hasRelatedWork W2016781223 @default.
- W2149656347 hasRelatedWork W2038545176 @default.
- W2149656347 hasRelatedWork W2076662319 @default.
- W2149656347 hasRelatedWork W2079725596 @default.
- W2149656347 hasRelatedWork W2315765065 @default.
- W2149656347 hasRelatedWork W2414153668 @default.
- W2149656347 hasRelatedWork W2731465304 @default.
- W2149656347 hasRelatedWork W77405950 @default.
- W2149656347 hasVolume "45" @default.
- W2149656347 isParatext "false" @default.
- W2149656347 isRetracted "false" @default.
- W2149656347 magId "2149656347" @default.
- W2149656347 workType "article" @default.