FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149660167> ?p ?o ?g. }

• W2149660167 endingPage "9" @default.
• W2149660167 startingPage "1121" @default.
• W2149660167 abstract "Identification and characterization of membrane proteins is a crucial challenge in proteomics research. Thus, we designed a novel method to prepare proteins possessing extensive hydrophobic stretches for mass spectrometry studies, without sacrificing other classes of proteins.This method uses sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) separation and relies solely on a matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) instrument, the most common and easiest to use mass spectrometer.Using this analytical procedure, a significant number of hydrophobic peptides were recovered, with no reduction in overall sequence coverage and with a good identification of transmembrane proteins sequence. Applying this method to the systematic identification of proteins located in lipid rafts, up to 47% of identified proteins were obtained with an improvement of sequence coverage.The procedure presented here is suitable for both identifying purified hydrophobic proteins and systematically investigating hydrophobic protein mixtures. It can be easily applied even in non-dedicated laboratories, such as those mostly devoted to clinical chemistry." @default.
• W2149660167 created "2016-06-24" @default.
• W2149660167 creator A5011098207 @default.
• W2149660167 creator A5012192966 @default.
• W2149660167 creator A5012377442 @default.
• W2149660167 creator A5061845168 @default.
• W2149660167 creator A5087184743 @default.
• W2149660167 date "2010-04-01" @default.
• W2149660167 modified "2023-09-24" @default.
• W2149660167 title "A simple and effective method to analyze membrane proteins by SDS-PAGE and MALDI mass spectrometry." @default.
• W2149660167 cites W1973791258 @default.
• W2149660167 cites W1991366673 @default.
• W2149660167 cites W2030227588 @default.
• W2149660167 cites W2049237667 @default.
• W2149660167 cites W2053315960 @default.
• W2149660167 cites W2053988697 @default.
• W2149660167 cites W2097648928 @default.
• W2149660167 cites W2119464357 @default.
• W2149660167 cites W2134852489 @default.
• W2149660167 cites W2137120403 @default.
• W2149660167 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20530417" @default.
• W2149660167 hasPublicationYear "2010" @default.
• W2149660167 type Work @default.
• W2149660167 sameAs 2149660167 @default.
• W2149660167 citedByCount "5" @default.
• W2149660167 countsByYear W21496601672013 @default.
• W2149660167 countsByYear W21496601672014 @default.
• W2149660167 countsByYear W21496601672017 @default.
• W2149660167 countsByYear W21496601672022 @default.
• W2149660167 crossrefType "journal-article" @default.
• W2149660167 hasAuthorship W2149660167A5011098207 @default.
• W2149660167 hasAuthorship W2149660167A5012192966 @default.
• W2149660167 hasAuthorship W2149660167A5012377442 @default.
• W2149660167 hasAuthorship W2149660167A5061845168 @default.
• W2149660167 hasAuthorship W2149660167A5087184743 @default.
• W2149660167 hasConcept C101660505 @default.
• W2149660167 hasConcept C104317684 @default.
• W2149660167 hasConcept C112979427 @default.
• W2149660167 hasConcept C116084860 @default.
• W2149660167 hasConcept C144647389 @default.
• W2149660167 hasConcept C150394285 @default.
• W2149660167 hasConcept C161200384 @default.
• W2149660167 hasConcept C162356407 @default.
• W2149660167 hasConcept C162711632 @default.
• W2149660167 hasConcept C170493617 @default.
• W2149660167 hasConcept C178790620 @default.
• W2149660167 hasConcept C181199279 @default.
• W2149660167 hasConcept C185592680 @default.
• W2149660167 hasConcept C202157981 @default.
• W2149660167 hasConcept C24530287 @default.
• W2149660167 hasConcept C31827203 @default.
• W2149660167 hasConcept C41625074 @default.
• W2149660167 hasConcept C43617362 @default.
• W2149660167 hasConcept C46111723 @default.
• W2149660167 hasConcept C55493867 @default.
• W2149660167 hasConcept C75280812 @default.
• W2149660167 hasConceptScore W2149660167C101660505 @default.
• W2149660167 hasConceptScore W2149660167C104317684 @default.
• W2149660167 hasConceptScore W2149660167C112979427 @default.
• W2149660167 hasConceptScore W2149660167C116084860 @default.
• W2149660167 hasConceptScore W2149660167C144647389 @default.
• W2149660167 hasConceptScore W2149660167C150394285 @default.
• W2149660167 hasConceptScore W2149660167C161200384 @default.
• W2149660167 hasConceptScore W2149660167C162356407 @default.
• W2149660167 hasConceptScore W2149660167C162711632 @default.
• W2149660167 hasConceptScore W2149660167C170493617 @default.
• W2149660167 hasConceptScore W2149660167C178790620 @default.
• W2149660167 hasConceptScore W2149660167C181199279 @default.
• W2149660167 hasConceptScore W2149660167C185592680 @default.
• W2149660167 hasConceptScore W2149660167C202157981 @default.
• W2149660167 hasConceptScore W2149660167C24530287 @default.
• W2149660167 hasConceptScore W2149660167C31827203 @default.
• W2149660167 hasConceptScore W2149660167C41625074 @default.
• W2149660167 hasConceptScore W2149660167C43617362 @default.
• W2149660167 hasConceptScore W2149660167C46111723 @default.
• W2149660167 hasConceptScore W2149660167C55493867 @default.
• W2149660167 hasConceptScore W2149660167C75280812 @default.
• W2149660167 hasIssue "4" @default.
• W2149660167 hasLocation W21496601671 @default.
• W2149660167 hasOpenAccess W2149660167 @default.
• W2149660167 hasPrimaryLocation W21496601671 @default.
• W2149660167 hasRelatedWork W1163034040 @default.
• W2149660167 hasRelatedWork W1601366267 @default.
• W2149660167 hasRelatedWork W1965417573 @default.
• W2149660167 hasRelatedWork W1987767012 @default.
• W2149660167 hasRelatedWork W1995964757 @default.
• W2149660167 hasRelatedWork W1996914970 @default.
• W2149660167 hasRelatedWork W1997081866 @default.
• W2149660167 hasRelatedWork W2035513313 @default.
• W2149660167 hasRelatedWork W2083076781 @default.
• W2149660167 hasRelatedWork W2134852489 @default.
• W2149660167 hasRelatedWork W2160007089 @default.
• W2149660167 hasRelatedWork W2353530743 @default.
• W2149660167 hasRelatedWork W2396361837 @default.
• W2149660167 hasRelatedWork W2413099574 @default.
• W2149660167 hasRelatedWork W2414312734 @default.
• W2149660167 hasRelatedWork W2963787867 @default.
• W2149660167 hasRelatedWork W68814561 @default.

• next