Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149667066> ?p ?o ?g. }
- W2149667066 endingPage "20943" @default.
- W2149667066 startingPage "20943" @default.
- W2149667066 abstract "We describe the synthesis and properties of five dinucleotide fluorescent cap analogues labelled at the ribose of the 7-methylguanosine moiety with either anthraniloyl (Ant) or N-methylanthraniloyl (Mant), which have been designed for the preparation of fluorescent mRNAs via transcription in vitro. Two of the analogues bear a methylene modification in the triphosphate bridge, providing resistance against either the Dcp2 or DcpS decapping enzymes. All these compounds were prepared by ZnCl2-mediated coupling of a nucleotide P-imidazolide with a fluorescently labelled mononucleotide. To evaluate the utility of these compounds for studying interactions with cap-binding proteins and cap-related cellular processes, both biological and spectroscopic features of those compounds were determined. The results indicate acceptable quantum yields of fluorescence, pH independence, environmental sensitivity, and photostability. The cap analogues are incorporated by RNA polymerase into mRNA transcripts that are efficiently translated in vitro. Transcripts containing fluorescent caps but unmodified in the triphosphate chain are hydrolysed by Dcp2 whereas those containing a α-β methylene modification are resistant. Model studies exploiting sensitivity of Mant to changes of local environment demonstrated utility of the synthesized compounds for studying cap-related proteins." @default.
- W2149667066 created "2016-06-24" @default.
- W2149667066 creator A5000185554 @default.
- W2149667066 creator A5012436300 @default.
- W2149667066 creator A5022730107 @default.
- W2149667066 creator A5027301759 @default.
- W2149667066 creator A5040441436 @default.
- W2149667066 creator A5057179851 @default.
- W2149667066 creator A5076498595 @default.
- W2149667066 creator A5085621206 @default.
- W2149667066 date "2013-01-01" @default.
- W2149667066 modified "2023-10-13" @default.
- W2149667066 title "Synthesis and evaluation of fluorescent cap analogues for mRNA labelling" @default.
- W2149667066 cites W1487314244 @default.
- W2149667066 cites W1509502060 @default.
- W2149667066 cites W1639795491 @default.
- W2149667066 cites W1847886362 @default.
- W2149667066 cites W1895248645 @default.
- W2149667066 cites W1970517901 @default.
- W2149667066 cites W1970986586 @default.
- W2149667066 cites W1972197294 @default.
- W2149667066 cites W1974280022 @default.
- W2149667066 cites W1976118264 @default.
- W2149667066 cites W1976456179 @default.
- W2149667066 cites W1979914470 @default.
- W2149667066 cites W1985324625 @default.
- W2149667066 cites W1996160970 @default.
- W2149667066 cites W1997012332 @default.
- W2149667066 cites W1997806846 @default.
- W2149667066 cites W1997930953 @default.
- W2149667066 cites W1998948607 @default.
- W2149667066 cites W1999143441 @default.
- W2149667066 cites W2001369160 @default.
- W2149667066 cites W2004352744 @default.
- W2149667066 cites W2007084777 @default.
- W2149667066 cites W2007704891 @default.
- W2149667066 cites W2008834682 @default.
- W2149667066 cites W2012530253 @default.
- W2149667066 cites W2014051254 @default.
- W2149667066 cites W2014255516 @default.
- W2149667066 cites W2023062743 @default.
- W2149667066 cites W2023728920 @default.
- W2149667066 cites W2032305454 @default.
- W2149667066 cites W2033019712 @default.
- W2149667066 cites W2034554412 @default.
- W2149667066 cites W2037313242 @default.
- W2149667066 cites W2048342396 @default.
- W2149667066 cites W2049898404 @default.
- W2149667066 cites W2051039710 @default.
- W2149667066 cites W2052888487 @default.
- W2149667066 cites W2055248630 @default.
- W2149667066 cites W2055627635 @default.
- W2149667066 cites W2057380385 @default.
- W2149667066 cites W2057666290 @default.
- W2149667066 cites W2059993444 @default.
- W2149667066 cites W2061822610 @default.
- W2149667066 cites W2063877028 @default.
- W2149667066 cites W2065104935 @default.
- W2149667066 cites W2066044406 @default.
- W2149667066 cites W2067329831 @default.
- W2149667066 cites W2074755975 @default.
- W2149667066 cites W2075181140 @default.
- W2149667066 cites W2080869576 @default.
- W2149667066 cites W2082939741 @default.
- W2149667066 cites W2085865810 @default.
- W2149667066 cites W2094182822 @default.
- W2149667066 cites W2109615349 @default.
- W2149667066 cites W2112185192 @default.
- W2149667066 cites W2115512260 @default.
- W2149667066 cites W2117767097 @default.
- W2149667066 cites W2122359996 @default.
- W2149667066 cites W2123848032 @default.
- W2149667066 cites W2126865761 @default.
- W2149667066 cites W2130779507 @default.
- W2149667066 cites W2134601150 @default.
- W2149667066 cites W2137998681 @default.
- W2149667066 cites W2157456092 @default.
- W2149667066 cites W2159638567 @default.
- W2149667066 cites W2165372505 @default.
- W2149667066 cites W2166457914 @default.
- W2149667066 cites W2167892056 @default.
- W2149667066 cites W2953126185 @default.
- W2149667066 doi "https://doi.org/10.1039/c3ra42769b" @default.
- W2149667066 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3834968" @default.
- W2149667066 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24273643" @default.
- W2149667066 hasPublicationYear "2013" @default.
- W2149667066 type Work @default.
- W2149667066 sameAs 2149667066 @default.
- W2149667066 citedByCount "22" @default.
- W2149667066 countsByYear W21496670662014 @default.
- W2149667066 countsByYear W21496670662015 @default.
- W2149667066 countsByYear W21496670662016 @default.
- W2149667066 countsByYear W21496670662017 @default.
- W2149667066 countsByYear W21496670662019 @default.
- W2149667066 countsByYear W21496670662020 @default.
- W2149667066 countsByYear W21496670662021 @default.
- W2149667066 countsByYear W21496670662022 @default.
- W2149667066 countsByYear W21496670662023 @default.