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- W2149689555 abstract "Abstract Fresh grapes and grape products, such as grape wine and grape juice, were analyzed for proanthocyanidins (PACs) using liquid chromatography with electrospray ionization mass spectrometric (MS) detection. PACs were successfully separated and analyzed on the basis of their protonated molecules, allowing the identification of PACs in different degrees of polymerization from monomers to oligomers (up to 7 units), and in various isomeric forms. Using reversed‐phase high‐performance liquid chromatography (HPLC) combined with MS detection, the PAC monomers, (+)‐catechin (C), (−)‐epicatechin (EC), (−)‐catechin gallate (CG), and (−)‐epicatechin gallate (ECG), were successfully quantified using selected ion monitoring (SIM) mode. Standard curves were fitted for each PAC ranging from 43.8 to 5600 ng/mL for C, from 42.2 to 5400 ng/mL for EC, from 36.7 to 4700 ng/mL for CG, and from 39.8 to 5100 ng/mL for ECG. Good linearity ( r 2 > 0.999) was achieved for each analyte. The accuracy and precision (RSD) were within 10% ( n = 8) at the limit of detection. This method allows direct quantification of monomeric PACs in fresh grapes and grape‐derived products. Additionally, flow injection analysis (FIA) was applied to estimate the concentration levels of PAC oligomers by comparing their FIA‐MS peak areas, which were well correlated ( r 2 = 0.936) to the total concentrations of PAC monomers. Copyright © 2005 John Wiley & Sons, Ltd." @default.
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- W2149689555 date "2005-06-29" @default.
- W2149689555 modified "2023-10-16" @default.
- W2149689555 title "Determination of proanthocyanidins in fresh grapes and grape products using liquid chromatography with mass spectrometric detection" @default.
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- W2149689555 doi "https://doi.org/10.1002/rcm.2029" @default.
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