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- W2149695505 abstract "Summary Borrelia burgdorferi surface lipoproteins are essential to the pathogenesis of Lyme borreliosis, but the mechanisms responsible for their localization are only beginning to emerge. We have previously demonstrated the critical nature of the amino‐terminal ‘tether’ domain of the mature lipoprotein for sorting a fluorescent reporter to the Borrelia cell surface. Here, we show that individual deletion of four contiguous residues within the tether of major surface lipoprotein OspA results in its inefficient translocation across the Borrelia outer membrane. Intriguingly, C‐terminal epitope tags of these N‐terminal deletion mutants were selectively surface‐exposed. Fold‐destabilizing C‐terminal point mutations and deletions did not block OspA secretion, but rather restored one of the otherwise periplasmic tether mutants to the bacterial surface. Together, these data indicate that disturbance of a confined tether feature leads to premature folding of OspA in the periplasm and thereby prevents secretion through the outer membrane. Furthermore, they suggest that OspA emerges tail‐first on the bacterial surface, yet independent of a specific C‐terminal targeting peptide sequence." @default.
- W2149695505 created "2016-06-24" @default.
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- W2149695505 creator A5019976375 @default.
- W2149695505 creator A5076498651 @default.
- W2149695505 creator A5087103832 @default.
- W2149695505 date "2010-05-21" @default.
- W2149695505 modified "2023-10-16" @default.
- W2149695505 title "Translocation of Borrelia burgdorferi surface lipoprotein OspA through the outer membrane requires an unfolded conformation and can initiate at the C-terminus" @default.
- W2149695505 cites W1519968567 @default.
- W2149695505 cites W156135585 @default.
- W2149695505 cites W1602952910 @default.
- W2149695505 cites W1608403029 @default.
- W2149695505 cites W1650944514 @default.
- W2149695505 cites W1662846517 @default.
- W2149695505 cites W1725484422 @default.
- W2149695505 cites W1792685479 @default.
- W2149695505 cites W1823757987 @default.
- W2149695505 cites W1879772459 @default.
- W2149695505 cites W1908391556 @default.
- W2149695505 cites W1912214366 @default.
- W2149695505 cites W1926303070 @default.
- W2149695505 cites W1949848418 @default.
- W2149695505 cites W1952225245 @default.
- W2149695505 cites W1954073637 @default.
- W2149695505 cites W1963621030 @default.
- W2149695505 cites W1965243248 @default.
- W2149695505 cites W1967946321 @default.
- W2149695505 cites W1974068054 @default.
- W2149695505 cites W1975804408 @default.
- W2149695505 cites W1978574783 @default.
- W2149695505 cites W1980427104 @default.
- W2149695505 cites W1983072845 @default.
- W2149695505 cites W1987494296 @default.
- W2149695505 cites W1997698750 @default.
- W2149695505 cites W2003381825 @default.
- W2149695505 cites W2010712022 @default.
- W2149695505 cites W2019956959 @default.
- W2149695505 cites W2021489665 @default.
- W2149695505 cites W2024876193 @default.
- W2149695505 cites W2025096364 @default.
- W2149695505 cites W2029820034 @default.
- W2149695505 cites W2034331079 @default.
- W2149695505 cites W2037279684 @default.
- W2149695505 cites W2045862160 @default.
- W2149695505 cites W2055401750 @default.
- W2149695505 cites W2058455674 @default.
- W2149695505 cites W2078048665 @default.
- W2149695505 cites W2088089656 @default.
- W2149695505 cites W2088837736 @default.
- W2149695505 cites W2094203715 @default.
- W2149695505 cites W2104128981 @default.
- W2149695505 cites W2104373250 @default.
- W2149695505 cites W2104484012 @default.
- W2149695505 cites W2105281406 @default.
- W2149695505 cites W2105332737 @default.
- W2149695505 cites W2106867194 @default.
- W2149695505 cites W2107853053 @default.
- W2149695505 cites W2107913200 @default.
- W2149695505 cites W2107943123 @default.
- W2149695505 cites W2109864391 @default.
- W2149695505 cites W2111485154 @default.
- W2149695505 cites W2113499624 @default.
- W2149695505 cites W2114455781 @default.
- W2149695505 cites W2114574473 @default.
- W2149695505 cites W2118783042 @default.
- W2149695505 cites W2119069290 @default.
- W2149695505 cites W2120197945 @default.
- W2149695505 cites W2120652759 @default.
- W2149695505 cites W2121303975 @default.
- W2149695505 cites W2129004786 @default.
- W2149695505 cites W2131492700 @default.
- W2149695505 cites W2131784297 @default.
- W2149695505 cites W2134759004 @default.
- W2149695505 cites W2140791138 @default.
- W2149695505 cites W2144384897 @default.
- W2149695505 cites W2144447139 @default.
- W2149695505 cites W2144549027 @default.
- W2149695505 cites W2145741346 @default.
- W2149695505 cites W2150712367 @default.
- W2149695505 cites W2151239002 @default.
- W2149695505 cites W2151408783 @default.
- W2149695505 cites W2152933863 @default.
- W2149695505 cites W2155189175 @default.
- W2149695505 cites W2158550334 @default.
- W2149695505 cites W2162994981 @default.
- W2149695505 cites W2165832456 @default.
- W2149695505 cites W2166905169 @default.
- W2149695505 cites W2168162332 @default.
- W2149695505 cites W2320088211 @default.
- W2149695505 cites W2320518687 @default.
- W2149695505 cites W2615823793 @default.
- W2149695505 doi "https://doi.org/10.1111/j.1365-2958.2010.07172.x" @default.
- W2149695505 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2999405" @default.
- W2149695505 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20398211" @default.
- W2149695505 hasPublicationYear "2010" @default.
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