FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149753834> ?p ?o ?g. }

• W2149753834 endingPage "902" @default.
• W2149753834 startingPage "889" @default.
• W2149753834 abstract "Substantial evidence implicates the phosphoinositide cascade in invertebrate phototransduction, but the final pathway of excitation remains obscure. In order to test the hypothesis that Ca2+ is the excitatory messenger rapid concentration jumps of cytosolic Ca2+ were achieved in dissociated Drosophila photoreceptors by flash photolysis of the caged Ca2+ compounds DM-nitrophen and nitr-5. Both compounds were introduced via patch pipettes used to record whole-cell currents. Calibrations using INDO-1 and Mag-INDO-1 indicated that photolysis of DM-nitrophen (5 mM loaded with 4 mM Ca2+), raised Ca, to ca. 20-50 microM, and nitr-5 (same loading) to ca. 1-2 microM. In mutants lacking light responses (ora, lacking rhodopsin; norpA, lacking phospholipase C; trp, which is inactivated by conditioning lights), the only current evoked by photolysis of DM-nitrophen was a small inward current with no detectable latency. This current did not reverse at +80 mV and was blocked by substitution of external Na+ for Li+, suggesting it represents activation of an electrogenic Na+/Ca2+ exchanger. A similar current was also the only current elicited by caged Ca2+ during the 5 msec latent period in wild type (WT) photoreceptors. To investigate possible modulatory effects of caged Ca2+ on the light-activated conductance, cells were first stimulated with a saturating light stimulus, itself incapable of releasing significant Ca2+, and then the photolytic flash was discharged during the response. During the rising phase of the response, photolysis of DM-nitrophen (but not nitr-5) induced a pronounced facilitation in WT photoreceptors. When photolysed during the plateau phase both DM-nitrophen and nitr-5 induced a rapid inactivation of the light-induced current. By contrast, in trp photoreceptors, which lack one class of Ca2+ permeable light-sensitive channel, photolysis of DM-nitrophen induced a significant facilitation during the falling phase of the response, but during the rising phase photolysis significantly depressed the overall response. In conclusion, caged Ca2+ failed to activate any channels in Drosophila photoreceptors but profoundly affected the light-dependent channels once they have been activated." @default.
• W2149753834 created "2016-06-24" @default.
• W2149753834 creator A5090506553 @default.
• W2149753834 date "1995-01-01" @default.
• W2149753834 modified "2023-10-18" @default.
• W2149753834 title "Photolysis of caged Ca2+ facilitates and inactivates but does not directly excite light-sensitive channels in Drosophila photoreceptors" @default.
• W2149753834 cites W1513366224 @default.
• W2149753834 cites W1956148136 @default.
• W2149753834 cites W1964723257 @default.
• W2149753834 cites W1966131633 @default.
• W2149753834 cites W1971445812 @default.
• W2149753834 cites W1974978517 @default.
• W2149753834 cites W1975483547 @default.
• W2149753834 cites W1980166477 @default.
• W2149753834 cites W1983127589 @default.
• W2149753834 cites W1994858599 @default.
• W2149753834 cites W1995047705 @default.
• W2149753834 cites W1997778815 @default.
• W2149753834 cites W2006842412 @default.
• W2149753834 cites W2015122100 @default.
• W2149753834 cites W2023025537 @default.
• W2149753834 cites W2033091999 @default.
• W2149753834 cites W2035493923 @default.
• W2149753834 cites W2037430823 @default.
• W2149753834 cites W2044354507 @default.
• W2149753834 cites W2047980386 @default.
• W2149753834 cites W2053008485 @default.
• W2149753834 cites W2054911978 @default.
• W2149753834 cites W2067109101 @default.
• W2149753834 cites W2073293904 @default.
• W2149753834 cites W2086755620 @default.
• W2149753834 cites W2087762065 @default.
• W2149753834 cites W2091368852 @default.
• W2149753834 cites W2102141265 @default.
• W2149753834 cites W2111629985 @default.
• W2149753834 cites W2114332687 @default.
• W2149753834 cites W2124923401 @default.
• W2149753834 cites W2133687351 @default.
• W2149753834 cites W2135976269 @default.
• W2149753834 cites W2136508296 @default.
• W2149753834 cites W2147914782 @default.
• W2149753834 cites W2150691484 @default.
• W2149753834 cites W2151590637 @default.
• W2149753834 cites W2152203056 @default.
• W2149753834 cites W2157892196 @default.
• W2149753834 cites W2164150184 @default.
• W2149753834 cites W2169873791 @default.
• W2149753834 cites W2415134306 @default.
• W2149753834 cites W2487989300 @default.
• W2149753834 doi "https://doi.org/10.1523/jneurosci.15-01-00889.1995" @default.
• W2149753834 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6578327" @default.
• W2149753834 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7529832" @default.
• W2149753834 hasPublicationYear "1995" @default.
• W2149753834 type Work @default.
• W2149753834 sameAs 2149753834 @default.
• W2149753834 citedByCount "108" @default.
• W2149753834 countsByYear W21497538342012 @default.
• W2149753834 countsByYear W21497538342013 @default.
• W2149753834 countsByYear W21497538342014 @default.
• W2149753834 countsByYear W21497538342015 @default.
• W2149753834 countsByYear W21497538342017 @default.
• W2149753834 countsByYear W21497538342018 @default.
• W2149753834 countsByYear W21497538342019 @default.
• W2149753834 countsByYear W21497538342021 @default.
• W2149753834 crossrefType "journal-article" @default.
• W2149753834 hasAuthorship W2149753834A5090506553 @default.
• W2149753834 hasBestOaLocation W21497538341 @default.
• W2149753834 hasConcept C112592302 @default.
• W2149753834 hasConcept C121332964 @default.
• W2149753834 hasConcept C12554922 @default.
• W2149753834 hasConcept C131667965 @default.
• W2149753834 hasConcept C13472781 @default.
• W2149753834 hasConcept C148898269 @default.
• W2149753834 hasConcept C163235415 @default.
• W2149753834 hasConcept C170493617 @default.
• W2149753834 hasConcept C181911157 @default.
• W2149753834 hasConcept C185592680 @default.
• W2149753834 hasConcept C202033177 @default.
• W2149753834 hasConcept C2780827179 @default.
• W2149753834 hasConcept C54582936 @default.
• W2149753834 hasConcept C55493867 @default.
• W2149753834 hasConcept C62520636 @default.
• W2149753834 hasConcept C75473681 @default.
• W2149753834 hasConcept C79879829 @default.
• W2149753834 hasConcept C86803240 @default.
• W2149753834 hasConcept C93391505 @default.
• W2149753834 hasConceptScore W2149753834C112592302 @default.
• W2149753834 hasConceptScore W2149753834C121332964 @default.
• W2149753834 hasConceptScore W2149753834C12554922 @default.
• W2149753834 hasConceptScore W2149753834C131667965 @default.
• W2149753834 hasConceptScore W2149753834C13472781 @default.
• W2149753834 hasConceptScore W2149753834C148898269 @default.
• W2149753834 hasConceptScore W2149753834C163235415 @default.
• W2149753834 hasConceptScore W2149753834C170493617 @default.
• W2149753834 hasConceptScore W2149753834C181911157 @default.
• W2149753834 hasConceptScore W2149753834C185592680 @default.
• W2149753834 hasConceptScore W2149753834C202033177 @default.
• W2149753834 hasConceptScore W2149753834C2780827179 @default.

• next