FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149761903> ?p ?o ?g. }

• W2149761903 endingPage "968" @default.
• W2149761903 startingPage "959" @default.
• W2149761903 abstract "Fluorogenic substrates [Ala-Pro](2)-cresyl violet and Ala-Pro-rhodamine 110 have been tested for microscopic detection of protease activity of dipeptidyl peptidase IV (DPPIV) in living cells. DPPIV activity is one of the many functions of the multifunctional or moonlighting protein CD26/DPPIV. As a model we used Jurkat cells, which are T-cells that lack CD26/DPPIV expression, and CD26/DPPIV-transfected Jurkat cells. Ala-Pro-rhodamine 110 is not fluorescent, but after proteolytic cleavage rhodamine 110 fluoresces. [Ala-Pro](2)-cresyl violet is fluorescent by itself but proteolytic cleavage into cresyl violet induces a shift to longer wavelengths. This phenomenon enables the simultaneous determination of local (intracellular) substrate and product concentrations, which is important for analysis of kinetics of the cleavage reaction. [Ala-Pro](2)-cresyl violet, but not Ala-Pro-rhodamine 110, appeared to be specific for DPPIV. When microscopic analysis is performed on living cells during the first minutes of the enzyme reaction, DPPIV activity can be precisely localized in cells with the use of [Ala-Pro](2)-cresyl violet. Fluorescent product is rapidly internalized into submembrane granules in transfected Jurkat cells and is redistributed intracellularly via internalization pathways that have been described for CD26/DPPIV. We conclude that [Ala-Pro](2)-cresyl violet is a good fluorogenic substrate to localize DPPIV activity in living cells when the correct wavelengths are used for excitation and emission and images are captured in the early stages of the enzyme reaction." @default.
• W2149761903 created "2016-06-24" @default.
• W2149761903 creator A5014768226 @default.
• W2149761903 creator A5015187758 @default.
• W2149761903 creator A5019641797 @default.
• W2149761903 creator A5085918635 @default.
• W2149761903 creator A5087147723 @default.
• W2149761903 date "2003-07-01" @default.
• W2149761903 modified "2023-10-03" @default.
• W2149761903 title "Fluorogenic Substrate [Ala-Pro]²-Cresyl Violet But Not Ala-Pro-Rhodamine 110 Is Cleaved Specifically by DPPIV Activity: A Study in Living Jurkat Cells and CD26/DPPIV-transfected Jurkat Cells" @default.
• W2149761903 cites W1223794020 @default.
• W2149761903 cites W1493627373 @default.
• W2149761903 cites W1505772652 @default.
• W2149761903 cites W1786608374 @default.
• W2149761903 cites W1982934705 @default.
• W2149761903 cites W2001304170 @default.
• W2149761903 cites W2009540691 @default.
• W2149761903 cites W2012252491 @default.
• W2149761903 cites W2015766339 @default.
• W2149761903 cites W2030213141 @default.
• W2149761903 cites W2044210567 @default.
• W2149761903 cites W2046370400 @default.
• W2149761903 cites W2049203358 @default.
• W2149761903 cites W2055665437 @default.
• W2149761903 cites W2059777229 @default.
• W2149761903 cites W2060481368 @default.
• W2149761903 cites W2064384444 @default.
• W2149761903 cites W2069244049 @default.
• W2149761903 cites W2069849774 @default.
• W2149761903 cites W2083035853 @default.
• W2149761903 cites W2091317550 @default.
• W2149761903 cites W2093536578 @default.
• W2149761903 cites W2095056873 @default.
• W2149761903 cites W2095753590 @default.
• W2149761903 cites W2099393212 @default.
• W2149761903 cites W2109871327 @default.
• W2149761903 cites W2114305848 @default.
• W2149761903 cites W2128126470 @default.
• W2149761903 cites W2158696091 @default.
• W2149761903 cites W2162061338 @default.
• W2149761903 cites W2168478301 @default.
• W2149761903 cites W2169004092 @default.
• W2149761903 cites W253129066 @default.
• W2149761903 cites W81574910 @default.
• W2149761903 doi "https://doi.org/10.1177/002215540305100711" @default.
• W2149761903 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12810846" @default.
• W2149761903 hasPublicationYear "2003" @default.
• W2149761903 type Work @default.
• W2149761903 sameAs 2149761903 @default.
• W2149761903 citedByCount "34" @default.
• W2149761903 countsByYear W21497619032012 @default.
• W2149761903 countsByYear W21497619032013 @default.
• W2149761903 countsByYear W21497619032016 @default.
• W2149761903 countsByYear W21497619032017 @default.
• W2149761903 countsByYear W21497619032018 @default.
• W2149761903 countsByYear W21497619032019 @default.
• W2149761903 countsByYear W21497619032021 @default.
• W2149761903 countsByYear W21497619032023 @default.
• W2149761903 crossrefType "journal-article" @default.
• W2149761903 hasAuthorship W2149761903A5014768226 @default.
• W2149761903 hasAuthorship W2149761903A5015187758 @default.
• W2149761903 hasAuthorship W2149761903A5019641797 @default.
• W2149761903 hasAuthorship W2149761903A5085918635 @default.
• W2149761903 hasAuthorship W2149761903A5087147723 @default.
• W2149761903 hasBestOaLocation W21497619031 @default.
• W2149761903 hasConcept C104317684 @default.
• W2149761903 hasConcept C121332964 @default.
• W2149761903 hasConcept C12554922 @default.
• W2149761903 hasConcept C153911025 @default.
• W2149761903 hasConcept C179464577 @default.
• W2149761903 hasConcept C185592680 @default.
• W2149761903 hasConcept C203014093 @default.
• W2149761903 hasConcept C2776090121 @default.
• W2149761903 hasConcept C2779740913 @default.
• W2149761903 hasConcept C2779916640 @default.
• W2149761903 hasConcept C54009773 @default.
• W2149761903 hasConcept C54355233 @default.
• W2149761903 hasConcept C55493867 @default.
• W2149761903 hasConcept C62520636 @default.
• W2149761903 hasConcept C74864618 @default.
• W2149761903 hasConcept C86803240 @default.
• W2149761903 hasConcept C8891405 @default.
• W2149761903 hasConcept C91881484 @default.
• W2149761903 hasConceptScore W2149761903C104317684 @default.
• W2149761903 hasConceptScore W2149761903C121332964 @default.
• W2149761903 hasConceptScore W2149761903C12554922 @default.
• W2149761903 hasConceptScore W2149761903C153911025 @default.
• W2149761903 hasConceptScore W2149761903C179464577 @default.
• W2149761903 hasConceptScore W2149761903C185592680 @default.
• W2149761903 hasConceptScore W2149761903C203014093 @default.
• W2149761903 hasConceptScore W2149761903C2776090121 @default.
• W2149761903 hasConceptScore W2149761903C2779740913 @default.
• W2149761903 hasConceptScore W2149761903C2779916640 @default.
• W2149761903 hasConceptScore W2149761903C54009773 @default.
• W2149761903 hasConceptScore W2149761903C54355233 @default.
• W2149761903 hasConceptScore W2149761903C55493867 @default.
• W2149761903 hasConceptScore W2149761903C62520636 @default.
• W2149761903 hasConceptScore W2149761903C74864618 @default.

• next