FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149783560> ?p ?o ?g. }

• W2149783560 endingPage "102" @default.
• W2149783560 startingPage "102" @default.
• W2149783560 abstract "Research using the zebrafish model has experienced a rapid growth in recent years. Although real-time reverse transcription PCR (QPCR), normalized to an internal reference (housekeeping) gene, is a frequently used method for quantifying gene expression changes in zebrafish, many commonly used housekeeping genes are known to vary with experimental conditions. To identify housekeeping genes that are stably expressed under different experimental conditions, and thus suitable as normalizers for QPCR in zebrafish, the present study evaluated the expression of eight commonly used housekeeping genes as a function of stage and hormone/toxicant exposure during development, and by tissue type and sex in adult fish.QPCR analysis was used to quantify mRNA levels of bactin1, tubulin alpha 1(tuba1), glyceraldehyde-3-phosphate dehydrogenase (gapdh), glucose-6-phosphate dehydrogenase (g6pd), TATA-box binding protein (tbp), beta-2-microglobulin (b2m), elongation factor 1 alpha (elfa), and 18s ribosomal RNA (18s) during development (2 - 120 hr postfertilization, hpf); in different tissue types (brain, eye, liver, heart, muscle, gonads) of adult males and females; and after treatment of embryos/larvae (24 - 96 hpf) with commonly used vehicles for administration and agents that represent known environmental endocrine disruptors. All genes were found to have some degree of variability under the conditions tested here. Rank ordering of expression stability using geNorm analysis identified 18s, b2m, and elfa as most stable during development and across tissue types, while gapdh, tuba1, and tpb were the most variable. Following chemical treatment, tuba1, bactin1, and elfa were the most stably expressed whereas tbp, 18s, and b2m were the least stable. Data also revealed sex differences that are gene- and tissue-specific, and treatment effects that are gene-, vehicle- and ligand-specific. When the accuracy of QPCR analysis was tested using different reference genes to measure suppression of cyp19a1b by an estrogen receptor antagonist and induction of cyp1a by an arylhydrocarbon receptor agonist, the direction and magnitude of effects with stable and unstable genes differed.This study provides data that can be expected to aid zebrafish researchers in their initial choice of housekeeping genes for future studies, but underlines the importance of further validating housekeeping genes for each new experimental paradigm and fish species." @default.
• W2149783560 created "2016-06-24" @default.
• W2149783560 creator A5019926661 @default.
• W2149783560 creator A5033293144 @default.
• W2149783560 date "2008-01-01" @default.
• W2149783560 modified "2023-10-17" @default.
• W2149783560 title "Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment" @default.
• W2149783560 cites W1563659068 @default.
• W2149783560 cites W1577577364 @default.
• W2149783560 cites W1977116995 @default.
• W2149783560 cites W1986080495 @default.
• W2149783560 cites W1998396050 @default.
• W2149783560 cites W2002534437 @default.
• W2149783560 cites W2002751444 @default.
• W2149783560 cites W2006523356 @default.
• W2149783560 cites W2016581179 @default.
• W2149783560 cites W2018438835 @default.
• W2149783560 cites W2021046584 @default.
• W2149783560 cites W2025358151 @default.
• W2149783560 cites W2025492442 @default.
• W2149783560 cites W2037659246 @default.
• W2149783560 cites W2057464020 @default.
• W2149783560 cites W2059702269 @default.
• W2149783560 cites W2060847230 @default.
• W2149783560 cites W2082933172 @default.
• W2149783560 cites W2088265690 @default.
• W2149783560 cites W2099824652 @default.
• W2149783560 cites W2104455852 @default.
• W2149783560 cites W2108768554 @default.
• W2149783560 cites W2109286101 @default.
• W2149783560 cites W2112982595 @default.
• W2149783560 cites W2117483311 @default.
• W2149783560 cites W2125795583 @default.
• W2149783560 cites W2126835198 @default.
• W2149783560 cites W2127535576 @default.
• W2149783560 cites W2127803065 @default.
• W2149783560 cites W2133948559 @default.
• W2149783560 cites W2136843378 @default.
• W2149783560 cites W2143146123 @default.
• W2149783560 cites W2145879504 @default.
• W2149783560 cites W2149799561 @default.
• W2149783560 cites W2156374900 @default.
• W2149783560 cites W2160085982 @default.
• W2149783560 cites W2173863177 @default.
• W2149783560 doi "https://doi.org/10.1186/1471-2199-9-102" @default.
• W2149783560 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2588455" @default.
• W2149783560 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19014500" @default.
• W2149783560 hasPublicationYear "2008" @default.
• W2149783560 type Work @default.
• W2149783560 sameAs 2149783560 @default.
• W2149783560 citedByCount "516" @default.
• W2149783560 countsByYear W21497835602012 @default.
• W2149783560 countsByYear W21497835602013 @default.
• W2149783560 countsByYear W21497835602014 @default.
• W2149783560 countsByYear W21497835602015 @default.
• W2149783560 countsByYear W21497835602016 @default.
• W2149783560 countsByYear W21497835602017 @default.
• W2149783560 countsByYear W21497835602018 @default.
• W2149783560 countsByYear W21497835602019 @default.
• W2149783560 countsByYear W21497835602020 @default.
• W2149783560 countsByYear W21497835602021 @default.
• W2149783560 countsByYear W21497835602022 @default.
• W2149783560 countsByYear W21497835602023 @default.
• W2149783560 crossrefType "journal-article" @default.
• W2149783560 hasAuthorship W2149783560A5019926661 @default.
• W2149783560 hasAuthorship W2149783560A5033293144 @default.
• W2149783560 hasBestOaLocation W21497835601 @default.
• W2149783560 hasConcept C104317684 @default.
• W2149783560 hasConcept C134792803 @default.
• W2149783560 hasConcept C150194340 @default.
• W2149783560 hasConcept C153911025 @default.
• W2149783560 hasConcept C16685009 @default.
• W2149783560 hasConcept C2776878037 @default.
• W2149783560 hasConcept C2779148672 @default.
• W2149783560 hasConcept C33922130 @default.
• W2149783560 hasConcept C48023723 @default.
• W2149783560 hasConcept C54355233 @default.
• W2149783560 hasConcept C71924100 @default.
• W2149783560 hasConcept C86803240 @default.
• W2149783560 hasConceptScore W2149783560C104317684 @default.
• W2149783560 hasConceptScore W2149783560C134792803 @default.
• W2149783560 hasConceptScore W2149783560C150194340 @default.
• W2149783560 hasConceptScore W2149783560C153911025 @default.
• W2149783560 hasConceptScore W2149783560C16685009 @default.
• W2149783560 hasConceptScore W2149783560C2776878037 @default.
• W2149783560 hasConceptScore W2149783560C2779148672 @default.
• W2149783560 hasConceptScore W2149783560C33922130 @default.
• W2149783560 hasConceptScore W2149783560C48023723 @default.
• W2149783560 hasConceptScore W2149783560C54355233 @default.
• W2149783560 hasConceptScore W2149783560C71924100 @default.
• W2149783560 hasConceptScore W2149783560C86803240 @default.
• W2149783560 hasIssue "1" @default.
• W2149783560 hasLocation W21497835601 @default.
• W2149783560 hasLocation W21497835602 @default.
• W2149783560 hasLocation W21497835603 @default.
• W2149783560 hasLocation W21497835604 @default.
• W2149783560 hasOpenAccess W2149783560 @default.
• W2149783560 hasPrimaryLocation W21497835601 @default.

• next