Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149789320> ?p ?o ?g. }
- W2149789320 endingPage "9231" @default.
- W2149789320 startingPage "9222" @default.
- W2149789320 abstract "The herpes simplex virus (HSV) virion host shutoff (vhs) protein (UL41 gene product) is a component of the HSV virion tegument that triggers shutoff of host protein synthesis and accelerated mRNA degradation during the early stages of HSV infection. vhs displays weak amino acid sequence similarity to the fen-1 family of nucleases and suffices to induce accelerated RNA turnover through endoribonucleolytic cleavage events when it is expressed as the only HSV protein in a rabbit reticulocyte in vitro translation system. Although vhs selectively targets mRNAs in vivo, the basis for this selectivity remains obscure, since in vitro activity is not influenced by the presence of a 5' cap or 3' poly(A) tail. Here we show that vhs activity is greatly altered by placing an internal ribosome entry site (IRES) from encephalomyocarditis virus or poliovirus in the RNA substrate. Transcripts bearing the IRES were preferentially cleaved by the vhs-dependent endoribonuclease at multiple sites clustered in a narrow zone located immediately downstream of the element in a reaction that did not require ribosomes. Targeting was observed when the IRES was located at the 5' end or placed at internal sites in the substrate, indicating that it is independent of position or sequence context. These data indicate that the vhs-dependent nuclease can be selectively targeted by specific cis-acting elements in the RNA substrate, possibly through secondary structure or a component of the translational machinery." @default.
- W2149789320 created "2016-06-24" @default.
- W2149789320 creator A5015898479 @default.
- W2149789320 creator A5052186763 @default.
- W2149789320 date "1999-11-01" @default.
- W2149789320 modified "2023-10-16" @default.
- W2149789320 title "Picornavirus Internal Ribosome Entry Site Elements Target RNA Cleavage Events Induced by the Herpes Simplex Virus Virion Host Shutoff Protein" @default.
- W2149789320 cites W100014517 @default.
- W2149789320 cites W116882698 @default.
- W2149789320 cites W1493069978 @default.
- W2149789320 cites W1509026992 @default.
- W2149789320 cites W1515161873 @default.
- W2149789320 cites W1520569049 @default.
- W2149789320 cites W1525567009 @default.
- W2149789320 cites W1539798432 @default.
- W2149789320 cites W1555772203 @default.
- W2149789320 cites W1576676044 @default.
- W2149789320 cites W1580270992 @default.
- W2149789320 cites W1584306191 @default.
- W2149789320 cites W1588939300 @default.
- W2149789320 cites W1606830172 @default.
- W2149789320 cites W1607579928 @default.
- W2149789320 cites W1627251303 @default.
- W2149789320 cites W1629488237 @default.
- W2149789320 cites W1629511537 @default.
- W2149789320 cites W1710344093 @default.
- W2149789320 cites W1741612611 @default.
- W2149789320 cites W1756256533 @default.
- W2149789320 cites W1773493432 @default.
- W2149789320 cites W1807212217 @default.
- W2149789320 cites W1824684517 @default.
- W2149789320 cites W1898229461 @default.
- W2149789320 cites W1911015508 @default.
- W2149789320 cites W1938197614 @default.
- W2149789320 cites W1938377731 @default.
- W2149789320 cites W1938755197 @default.
- W2149789320 cites W1971848160 @default.
- W2149789320 cites W1973433187 @default.
- W2149789320 cites W1974534167 @default.
- W2149789320 cites W1974868310 @default.
- W2149789320 cites W1976365049 @default.
- W2149789320 cites W1978202804 @default.
- W2149789320 cites W1983424753 @default.
- W2149789320 cites W1984734782 @default.
- W2149789320 cites W1990771642 @default.
- W2149789320 cites W2013284471 @default.
- W2149789320 cites W2015654553 @default.
- W2149789320 cites W2018289835 @default.
- W2149789320 cites W2022131014 @default.
- W2149789320 cites W2026073476 @default.
- W2149789320 cites W2026885676 @default.
- W2149789320 cites W2031061885 @default.
- W2149789320 cites W2033676950 @default.
- W2149789320 cites W2041762780 @default.
- W2149789320 cites W2046328332 @default.
- W2149789320 cites W2048731469 @default.
- W2149789320 cites W2048831848 @default.
- W2149789320 cites W2049747157 @default.
- W2149789320 cites W2062356878 @default.
- W2149789320 cites W2069652470 @default.
- W2149789320 cites W2086515928 @default.
- W2149789320 cites W2090441795 @default.
- W2149789320 cites W2097391157 @default.
- W2149789320 cites W2099136664 @default.
- W2149789320 cites W2100837269 @default.
- W2149789320 cites W2102754190 @default.
- W2149789320 cites W2104573851 @default.
- W2149789320 cites W2105568629 @default.
- W2149789320 cites W2107119800 @default.
- W2149789320 cites W2110615225 @default.
- W2149789320 cites W2120633733 @default.
- W2149789320 cites W2125207784 @default.
- W2149789320 cites W2147713929 @default.
- W2149789320 cites W2148729457 @default.
- W2149789320 cites W2161943011 @default.
- W2149789320 cites W2162279718 @default.
- W2149789320 cites W2162361253 @default.
- W2149789320 cites W2167039974 @default.
- W2149789320 cites W2168494912 @default.
- W2149789320 cites W2168803152 @default.
- W2149789320 cites W2576734941 @default.
- W2149789320 cites W4231701841 @default.
- W2149789320 cites W4246387564 @default.
- W2149789320 cites W69657478 @default.
- W2149789320 doi "https://doi.org/10.1128/jvi.73.11.9222-9231.1999" @default.
- W2149789320 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/112956" @default.
- W2149789320 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10516030" @default.
- W2149789320 hasPublicationYear "1999" @default.
- W2149789320 type Work @default.
- W2149789320 sameAs 2149789320 @default.
- W2149789320 citedByCount "60" @default.
- W2149789320 countsByYear W21497893202013 @default.
- W2149789320 countsByYear W21497893202014 @default.
- W2149789320 countsByYear W21497893202016 @default.
- W2149789320 countsByYear W21497893202018 @default.
- W2149789320 countsByYear W21497893202022 @default.
- W2149789320 crossrefType "journal-article" @default.
- W2149789320 hasAuthorship W2149789320A5015898479 @default.