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• W2149800141 abstract "The gene spoIIID, which is essential for spore formation in Bacillus subtilis, was cloned and sequenced. It consists of one open reading frame which would encode a 93-amino-acid protein with a classic helix-turn-helix motif, characteristic of sequence-specific DNA-binding proteins. SpoIIID protein is a previously identified transcription factor, capable of altering the specificity of RNA polymerase containing sigma K in vitro (Kroos et al., 1989). The spoIIID83 mutation (by which the locus was originally identified), was sequenced and found to be a single base substitution in the ribosome binding site upstream of the spoIIID open reading frame. A transcriptional fusion to lacZ was constructed and used to examine the regulation of spoIIID. Expression of spoIIID occurred only during sporulation, beginning 1.5 to 2 hours after the initiation of sporulation. The dependence of spoIIID expression on other spo loci suggests that it is mother-cell-specific, and that it is transcribed by sigma E-containing RNA polymerase." @default.
• W2149800141 created "2016-06-24" @default.
• W2149800141 creator A5033275201 @default.
• W2149800141 creator A5085131909 @default.
• W2149800141 date "1990-04-01" @default.
• W2149800141 modified "2023-09-26" @default.
• W2149800141 title "Differential gene expression during sporulation in Bacillus subtilis: structure and regulation of the spoIIID gene" @default.
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• W2149800141 doi "https://doi.org/10.1111/j.1365-2958.1990.tb00622.x" @default.
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