Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149889721> ?p ?o ?g. }
- W2149889721 abstract "Culex quinquefasciatus (Say) is a major species in the Culex pipiens complex and an important vector for several human pathogens including West Nile virus and parasitic filarial nematodes causing lymphatic filariasis. It is common throughout tropical and subtropical regions and is among the most geographically widespread mosquito species. Although the complete genome sequence is now available, additional genomic tools are needed to improve the sequence assembly. We constructed a bacterial artificial chromosome (BAC) library using the pIndigoBAC536 vector and Hin dIII partially digested DNA isolated from Cx. quinquefasciatus pupae, Johannesburg strain (NDJ). Insert size was estimated by Not I digestion and pulsed-field gel electrophoresis of 82 randomly selected clones. To estimate genome coverage, each 384-well plate was pooled for screening with 29 simple sequence repeat (SSR) and five gene markers. The NDJ library consists of 55,296 clones arrayed in 144 384-well microplates. Fragment insert size ranged from 50 to 190 kb in length (mean = 106 kb). Based on a mean insert size of 106 kb and a genome size of 579 Mbp, the BAC library provides ~10.1-fold coverage of the Cx. quinquefasciatus genome. PCR screening of BAC DNA plate pools for SSR loci from the genetic linkage map and for four genes associated with reproductive diapause in Culex pipiens resulted in a mean of 9.0 positive plate pools per locus. The NDJ library represents an excellent resource for genome assembly enhancement and characterization in Culex pipiens complex mosquitoes." @default.
- W2149889721 created "2016-06-24" @default.
- W2149889721 creator A5016848827 @default.
- W2149889721 creator A5043550732 @default.
- W2149889721 creator A5050245360 @default.
- W2149889721 creator A5053000890 @default.
- W2149889721 creator A5057795558 @default.
- W2149889721 creator A5076901564 @default.
- W2149889721 date "2011-09-13" @default.
- W2149889721 modified "2023-10-09" @default.
- W2149889721 title "Enhancing genome investigations in the mosquito Culex quinquefasciatus via BAC library construction and characterization" @default.
- W2149889721 cites W1610445311 @default.
- W2149889721 cites W1802048937 @default.
- W2149889721 cites W1834809368 @default.
- W2149889721 cites W1968266351 @default.
- W2149889721 cites W1974334580 @default.
- W2149889721 cites W1995077546 @default.
- W2149889721 cites W2018151612 @default.
- W2149889721 cites W2019410656 @default.
- W2149889721 cites W2025176542 @default.
- W2149889721 cites W2039358220 @default.
- W2149889721 cites W2044579583 @default.
- W2149889721 cites W2058193823 @default.
- W2149889721 cites W2059129968 @default.
- W2149889721 cites W2069492981 @default.
- W2149889721 cites W2070986542 @default.
- W2149889721 cites W2082612906 @default.
- W2149889721 cites W2089405975 @default.
- W2149889721 cites W2093503782 @default.
- W2149889721 cites W2117936709 @default.
- W2149889721 cites W2122657809 @default.
- W2149889721 cites W2126926648 @default.
- W2149889721 cites W2129652152 @default.
- W2149889721 cites W2131239006 @default.
- W2149889721 cites W2131641344 @default.
- W2149889721 cites W2133519870 @default.
- W2149889721 cites W2134551562 @default.
- W2149889721 cites W2135547884 @default.
- W2149889721 cites W2139642022 @default.
- W2149889721 cites W2141652419 @default.
- W2149889721 cites W2154066348 @default.
- W2149889721 cites W2154637292 @default.
- W2149889721 cites W2158009918 @default.
- W2149889721 cites W2158148243 @default.
- W2149889721 cites W2162801432 @default.
- W2149889721 cites W2168630377 @default.
- W2149889721 cites W2172967392 @default.
- W2149889721 cites W2178687352 @default.
- W2149889721 cites W2258800320 @default.
- W2149889721 cites W2340125979 @default.
- W2149889721 cites W292206659 @default.
- W2149889721 cites W4249330795 @default.
- W2149889721 cites W56346502 @default.
- W2149889721 doi "https://doi.org/10.1186/1756-0500-4-358" @default.
- W2149889721 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3180476" @default.
- W2149889721 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21914202" @default.
- W2149889721 hasPublicationYear "2011" @default.
- W2149889721 type Work @default.
- W2149889721 sameAs 2149889721 @default.
- W2149889721 citedByCount "7" @default.
- W2149889721 countsByYear W21498897212013 @default.
- W2149889721 countsByYear W21498897212014 @default.
- W2149889721 countsByYear W21498897212015 @default.
- W2149889721 countsByYear W21498897212016 @default.
- W2149889721 countsByYear W21498897212017 @default.
- W2149889721 crossrefType "journal-article" @default.
- W2149889721 hasAuthorship W2149889721A5016848827 @default.
- W2149889721 hasAuthorship W2149889721A5043550732 @default.
- W2149889721 hasAuthorship W2149889721A5050245360 @default.
- W2149889721 hasAuthorship W2149889721A5053000890 @default.
- W2149889721 hasAuthorship W2149889721A5057795558 @default.
- W2149889721 hasAuthorship W2149889721A5076901564 @default.
- W2149889721 hasBestOaLocation W21498897211 @default.
- W2149889721 hasConcept C104317684 @default.
- W2149889721 hasConcept C127413603 @default.
- W2149889721 hasConcept C13575015 @default.
- W2149889721 hasConcept C141231307 @default.
- W2149889721 hasConcept C173758957 @default.
- W2149889721 hasConcept C182901222 @default.
- W2149889721 hasConcept C2776217558 @default.
- W2149889721 hasConcept C2776442642 @default.
- W2149889721 hasConcept C2777775583 @default.
- W2149889721 hasConcept C2779518617 @default.
- W2149889721 hasConcept C2780098074 @default.
- W2149889721 hasConcept C3017666073 @default.
- W2149889721 hasConcept C54355233 @default.
- W2149889721 hasConcept C59822182 @default.
- W2149889721 hasConcept C61271108 @default.
- W2149889721 hasConcept C78519656 @default.
- W2149889721 hasConcept C86803240 @default.
- W2149889721 hasConceptScore W2149889721C104317684 @default.
- W2149889721 hasConceptScore W2149889721C127413603 @default.
- W2149889721 hasConceptScore W2149889721C13575015 @default.
- W2149889721 hasConceptScore W2149889721C141231307 @default.
- W2149889721 hasConceptScore W2149889721C173758957 @default.
- W2149889721 hasConceptScore W2149889721C182901222 @default.
- W2149889721 hasConceptScore W2149889721C2776217558 @default.
- W2149889721 hasConceptScore W2149889721C2776442642 @default.
- W2149889721 hasConceptScore W2149889721C2777775583 @default.
- W2149889721 hasConceptScore W2149889721C2779518617 @default.