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- W2150011619 abstract "Uridine kinase (UK) and uracil phosphoribosyltransferase (UPRT) are enzymes in pyrimidine salvage pathway. UK or UPRT were reported as separate genes in bacteria. Amino acid sequence of OsUK/UPRT1 from rice shows homology to bacterial enzymes. Amino-terminal region is similar to UDK with an ATP/GTP-binding motif, whereas the carboxyl-terminal similar to UPP with two binding motifs for a uracil and a PRPP, respectively. Expression of OsUK/UPRT1 in an udk and/or upp mutant of Escherichia coli led to growth inhibition with 5-fluorouridine or 5-fluorouracil. These results suggest that the OsUK/UPRT1 product would be a natural fusion protein of two enzymes. Key words: 5-Fluorouracil, 5-fluorouridine, Oryza sativa, uridine kinase, uracil phosphoribosyltransferase." @default.
- W2150011619 created "2016-06-24" @default.
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- W2150011619 date "2010-03-01" @default.
- W2150011619 modified "2023-09-27" @default.
- W2150011619 title "Expression of a natural fusion gene for uracil phosphoribosyltransferase and uridine kinase from rice shows growth retardation by 5-fluorouridine or 5-fluorouracil in Escherichia coli" @default.
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- W2150011619 doi "https://doi.org/10.5897/ajb10.1140" @default.
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