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• W2150069346 abstract "The RIE-1 cell line is an untransformed, epithelial cell line derived from the rat small intestine. We report that epidermal growth factor (EGF), which regulates the proliferation of RIE-1 cells, also directs their movement. We measured cell migration through gelatin-coated filters in blind-well Boyden chambers. The migration of RIE-1 cells was stimulated up to approximately 100-fold by EGF, with a half-maximal response at 1-2 ng/ml and a maximal effect at 10 ng/ml. Further analysis showed that the RIE-1 cells responded directionally to a gradient of EGF in solution. Other growth factors tested did not stimulate RIE-1 cell migration, and EGF did not stimulate the migration of fibroblasts in this assay. We conclude that EGF is a potent and specific chemo-attractant for RIE-1 intestinal epithelial cells and suggest that EGF might influence epithelial cell migration in vivo." @default.
• W2150069346 created "2016-06-24" @default.
• W2150069346 creator A5018660886 @default.
• W2150069346 creator A5085039504 @default.
• W2150069346 date "1985-07-01" @default.
• W2150069346 modified "2023-10-13" @default.
• W2150069346 title "Epidermal growth factor promotes the chemotactic migration of cultured rat intestinal epithelial cells" @default.
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• W2150069346 doi "https://doi.org/10.1002/jcp.1041240117" @default.
• W2150069346 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3876338" @default.
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