FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2150154631> ?p ?o ?g. }

• W2150154631 endingPage "1212" @default.
• W2150154631 startingPage "1196" @default.
• W2150154631 abstract "We examined the interaction of complement component C3 with surface molecules on Trypanosoma cruzi. Five- to six-fold more C3 was bound to epimastigotes (Epi) than to metacyclic trypomastigotes (CMT) of strain M88. Epi and CMT were surface iodinated, then incubated in C8-deficient serum, and detergent lysates were applied to anti-C3 antibody that had been coupled to Sepharose. We found that 9.20-10.24% of applied 125I-Epi protein bound to anti-C3-sepharose, compared to 2.64% binding of 125I-CMT protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that C3 was attached to 125I-Epi protein by a covalent bond. Samples eluted from anti-C3-sepharose with hydroxylamine revealed a single, major, 72 kD band, suggesting that C3b attaches almost exclusively to the 72 kD glycoprotein of Epi by a hydroxylamine-susceptible ester bond. An antiserum was prepared from lysates of serum-treated Epi that had been affinity-purified on anti-C3-sepharose. This antiserum immunoprecipitated a single 72 kD component (gp72) from surface-iodinated Epi, and specifically recognized only gp72 from Epi in immunoblots. In contrast to the results with Epi, gp72 on CMT was not found to be an efficient acceptor molecule for C3 deposition. The results are the first to evaluate the acceptor site for C3 deposition on a parasite, and they show that gp72 on Epi, but not gp72 on CMT, serves as the preferential acceptor for C3 during antibody-independent alternative complement pathway activation." @default.
• W2150154631 created "2016-06-24" @default.
• W2150154631 creator A5016487212 @default.
• W2150154631 creator A5043206312 @default.
• W2150154631 creator A5082148123 @default.
• W2150154631 creator A5084639477 @default.
• W2150154631 date "1985-05-01" @default.
• W2150154631 modified "2023-10-17" @default.
• W2150154631 title "gp72, the 72 kilodalton glycoprotein, is the membrane acceptor site for C3 on Trypanosoma cruzi epimastigotes." @default.
• W2150154631 cites W1484917094 @default.
• W2150154631 cites W1494339842 @default.
• W2150154631 cites W1494557099 @default.
• W2150154631 cites W1501060150 @default.
• W2150154631 cites W1502671196 @default.
• W2150154631 cites W1508553999 @default.
• W2150154631 cites W1515043523 @default.
• W2150154631 cites W1526898493 @default.
• W2150154631 cites W1527643370 @default.
• W2150154631 cites W1548937162 @default.
• W2150154631 cites W1589778844 @default.
• W2150154631 cites W1909117371 @default.
• W2150154631 cites W1943027424 @default.
• W2150154631 cites W1970276449 @default.
• W2150154631 cites W1973279449 @default.
• W2150154631 cites W1978006520 @default.
• W2150154631 cites W1979024863 @default.
• W2150154631 cites W1987486786 @default.
• W2150154631 cites W1992760440 @default.
• W2150154631 cites W1996075092 @default.
• W2150154631 cites W2003874206 @default.
• W2150154631 cites W2015760514 @default.
• W2150154631 cites W2018466824 @default.
• W2150154631 cites W2020469064 @default.
• W2150154631 cites W2025627503 @default.
• W2150154631 cites W2028299446 @default.
• W2150154631 cites W2031423487 @default.
• W2150154631 cites W2032568570 @default.
• W2150154631 cites W2047078732 @default.
• W2150154631 cites W2048960863 @default.
• W2150154631 cites W2056582402 @default.
• W2150154631 cites W2071190956 @default.
• W2150154631 cites W2101201405 @default.
• W2150154631 cites W2122656843 @default.
• W2150154631 cites W2141656646 @default.
• W2150154631 cites W2169194436 @default.
• W2150154631 cites W2378014634 @default.
• W2150154631 doi "https://doi.org/10.1084/jem.161.5.1196" @default.
• W2150154631 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2187612" @default.
• W2150154631 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3886827" @default.
• W2150154631 hasPublicationYear "1985" @default.
• W2150154631 type Work @default.
• W2150154631 sameAs 2150154631 @default.
• W2150154631 citedByCount "52" @default.
• W2150154631 countsByYear W21501546312013 @default.
• W2150154631 countsByYear W21501546312022 @default.
• W2150154631 crossrefType "journal-article" @default.
• W2150154631 hasAuthorship W2150154631A5016487212 @default.
• W2150154631 hasAuthorship W2150154631A5043206312 @default.
• W2150154631 hasAuthorship W2150154631A5082148123 @default.
• W2150154631 hasAuthorship W2150154631A5084639477 @default.
• W2150154631 hasBestOaLocation W21501546311 @default.
• W2150154631 hasConcept C108625454 @default.
• W2150154631 hasConcept C116084860 @default.
• W2150154631 hasConcept C136764020 @default.
• W2150154631 hasConcept C151730666 @default.
• W2150154631 hasConcept C153911025 @default.
• W2150154631 hasConcept C159654299 @default.
• W2150154631 hasConcept C179247698 @default.
• W2150154631 hasConcept C181199279 @default.
• W2150154631 hasConcept C185592680 @default.
• W2150154631 hasConcept C203014093 @default.
• W2150154631 hasConcept C2776487282 @default.
• W2150154631 hasConcept C2777625466 @default.
• W2150154631 hasConcept C2778913249 @default.
• W2150154631 hasConcept C2779881004 @default.
• W2150154631 hasConcept C41008148 @default.
• W2150154631 hasConcept C55493867 @default.
• W2150154631 hasConcept C71928629 @default.
• W2150154631 hasConcept C86803240 @default.
• W2150154631 hasConcept C91523082 @default.
• W2150154631 hasConceptScore W2150154631C108625454 @default.
• W2150154631 hasConceptScore W2150154631C116084860 @default.
• W2150154631 hasConceptScore W2150154631C136764020 @default.
• W2150154631 hasConceptScore W2150154631C151730666 @default.
• W2150154631 hasConceptScore W2150154631C153911025 @default.
• W2150154631 hasConceptScore W2150154631C159654299 @default.
• W2150154631 hasConceptScore W2150154631C179247698 @default.
• W2150154631 hasConceptScore W2150154631C181199279 @default.
• W2150154631 hasConceptScore W2150154631C185592680 @default.
• W2150154631 hasConceptScore W2150154631C203014093 @default.
• W2150154631 hasConceptScore W2150154631C2776487282 @default.
• W2150154631 hasConceptScore W2150154631C2777625466 @default.
• W2150154631 hasConceptScore W2150154631C2778913249 @default.
• W2150154631 hasConceptScore W2150154631C2779881004 @default.
• W2150154631 hasConceptScore W2150154631C41008148 @default.
• W2150154631 hasConceptScore W2150154631C55493867 @default.
• W2150154631 hasConceptScore W2150154631C71928629 @default.
• W2150154631 hasConceptScore W2150154631C86803240 @default.

• next