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- W2150205973 abstract "Difficulty may arise in distinguishing between ping-pong and sequential enzyme mechanisms, when using double-reciprocal plots of kinetic data obtained at several fixed values of the concentration of the second substrate; this is because the parallel lines diagnostic of the first case may actually be slowly converging as required by the second. An alternative approach is to choose substrate concentrations so that the second substrate concentration occurs in a number of fixed ratios to the first. A ping-pong mechanism predicts a set of straight lines and a sequential mechanism a set of curved lines (parabolas) in double-reciprocal plots. The method is illustrated by its application to bovine-milk lactose synthetase which requires UDP-galactose and N-acetyl-glucosamine as substrates in a sequential mechanism." @default.
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- W2150205973 date "1975-04-01" @default.
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- W2150205973 title "Kinetic Discrimination between Two Types of Enzyme Mechanism. Application to Lactose Synthetase" @default.
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- W2150205973 doi "https://doi.org/10.1111/j.1432-1033.1975.tb04057.x" @default.
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