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- W2150328730 abstract "The European corn borer (ECB) is a serious pest of corn causing significant yield losses in the Principal Investigator: Midwest. In continuous work on innovative control of the ECB via an entomopathogenic fungus, this project Leslie C. Lewis evaluated alternative carriers for delivery of Beauvaria bassiana without increasing indigenous mycotoxEntomology ins. Iowa State University Budget: $13,889 for year one $13,889 for year two Background The European corn borer (ECB) was first discovered in the United States in 1917 and in Iowa in 1942. Losses incurred from corn borer are estimated to rank second annually to the corn rootworm complex in Iowa. Estimated yield losses from ECB can range from $20 to $50 per acre in the Midwest. The corn borer has varying numbers of genera tions per growing season with range depend ing on day length, temperature, and genetic composition of the population. Iowa usually sees two generations of corn borers, with the first generation causing the characteristic “shot hole” damage on the whorl tissue. Beauveria bassiana, an entomopathogenic fun gus, occurs naturally in soil and plant residue and colonizes corn plant tissue. The term “endophyte” is used to describe a relationship between a fungus and a plant in which both organisms benefit from the relationship. B. bassiana is a unique fungus in that it is not only an effective pathogen of a wide variety of insect pests, but also able to form an endo phytic relationship with a wide variety of plants, the most economically important of which is corn. Because of its endophytic relationship, B. bassiana has been used experimentally as a plant protectant. The corn plant experi ences reduced insect attack and B. bassiana is able to propagate well in the moist, humid environment inside the corn plant. B. bassiana, applied in granular formulation during the whorl-stage of corn development, provides season-long control of corn borer populations. In addition, several liquid formu lations of B. bassiana are being experimen tally evaluated for their effectiveness in pro tecting corn plants from corn borer. Further advances in strain identification, production, and formulation will increase the effective ness of B. bassiana as a crop protectant and will give growers an alternative to synthetic insecticides for insect pest control. The objectives of these studies were to evalu ate alternative carriers for delivery of B. bassiana to whorl-stage corn for season-long suppression of the ECB, without increasing the amount of indigenous mycotoxins. Approach and methods In 1998 and 1999, the investigators conducted on-farm research with cooperators from the Practical Farmers of Iowa and an additional private farmer. Treatments consisted of five different B. bassiana carriers applied at a rate of 10 lb/acre and an untreated control. In an earlier study, the B. bassiana was formu lated on a corn kernel-based carrier. The EnviLeopold Center Progress Reports Volume 10 (2001) For more information contact Leslie Lewis, Entomology, Iowa State University, Ames, Iowa 50011; (515) 294-8614, e-mail leslewis@iastate.edu ronmental Protection Agency raised concerns that the use of this carrier might lead to el evated levels of mycotoxin from Aspergillus spp., which could be present in the carrier. To address this concern, four alternative carriers were evaluated to determine their efficacy in controlling ECB and their propensity to in crease levels of aflatoxin in grain. Carriers tested were the corn kernel based carrier, corn cob grit, clay, and the substrate used to grow the fungus ground to a 14/20 or 20/40 mesh. Granules were applied with an over-the-row applicator, with four to six repli cations per location. Entomological data on the efficacy of larval control from each treatment were collected at plant maturity by splitting 20 plants from tassel to base and determining the amount of corn borer larval feeding. The number and length of larval tunneling, number of larvae infected with B. bassiana, and the number of plants with no evidence of corn borer feeding were determined. At maturity, each plot was harvested and a weigh wagon was used to determine yields. Corn from each plot also was sampled for aflatoxin testing. Results and discussion Because of the wet spring in 1998, the inves tigators were unable to apply the B. bassiana at the most efficacious growth stage. Applica tion at the Bruck farm occurred at the proper time, but subsequent flooding of the field resulted in reduced stands and complete herbi cide failure. Applications at the other three locations were made past the proper growth stage, which reduced the effectiveness of the treatment. Time constraints in the fall did not allow collection of data from the Alert farm. In 1999, granules were applied at all locations at the proper growth stage and entomological and yield data also were obtained from all locations. Findings showed that there were alternatives to the corn kernel-based carrier that provide the same level of corn borer control without the worry of inadvertently elevating the afla toxin levels. The amount of corn borer pres sure was very light in 1998, and there were no consistent differences in reduction of corn borer feeding or significantly increased in yield compared with 1997 preliminary findings by the project investigators. Significant differ ences in corn borer feeding were observed only at locations with the highest levels of infestation during both years of the study. There were significant differences in 1998 yields at Cumming, Iowa, but no correspond ing differences in insect injury. The fact that two completely different sce narios played out emphasizes that insect injury is only one part of the yield equation. Injury from insects has to be quite severe before insect control measures result in a correspond ing increase in yield. Losses can still average up to 6 percent even if a chemical insecticide treatment is used. Results of testing showed the aflatoxin levels on all of the treatments at each location during both years were below the detectable level of 20 ppb, with the exception of one plot at Cumming where levels reached 55 ppb in 1999. (It is not uncommon to find a sample within a field with slightly elevated aflatoxin levels.) Therefore, based on these results and 1997 data in which there were no detectable levels of aflatoxin in preliminary work, all of the alternative carriers are suitable substitutes for the corn kernel-based carrier." @default.
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- W2150328730 date "2001-01-01" @default.
- W2150328730 modified "2023-09-27" @default.
- W2150328730 title "Development of alternative carriers for use of Beauveria Bassiana in Ostrinia nubilalis suppression on corn" @default.
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