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• W2150365819 startingPage "1047" @default.
• W2150365819 abstract "This work develops a fluorescence approach for sensitive detection of DNA methyltransferase activity based on endonuclease and rolling circle amplification (RCA) technique. In the presence of DNA adenine methylation (Dam) MTase, the methylation-responsive sequence of hairpin probe is methylated and cleaved by the methylation-sensitive restriction endonuclease Dpn I. The products cleaved by restriction endonuclease Dpn I then function as a signal primer to initiate RCA reaction by hybridizing with the circular DNA template. Each RCA product containing thousands of repeated sequences might hybridize with a large number of molecular beacons (detection probes), resulting in an enhanced fluorescence signal. In the absence of Dam MTase, neither methylation/cleavage nor RCA reaction can be initiated and no fluorescence signal is observed. The proposed method exhibits a dynamic range from 0.5 U/mL to 30 U/mL and a detection limit of 0.18 U/mL. This method can be used for the screening of antimicrobial drugs and has a great potential to be further applied in early clinical diagnosis." @default.
• W2150365819 created "2016-06-24" @default.
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• W2150365819 date "2014-07-01" @default.
• W2150365819 modified "2023-10-14" @default.
• W2150365819 title "Sensitive detection of DNA methyltransferase activity based on rolling circle amplification technology" @default.
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• W2150365819 doi "https://doi.org/10.1016/j.cclet.2014.05.002" @default.
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