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- W2150417688 endingPage "364" @default.
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- W2150417688 abstract "Methods such as monoclonal antibody technology, phage display, and ribosome display provide genetic routes to the selection of proteins and peptides with desired properties. However, extension to polymers of unnatural amino acids is problematic because the translation step is always performed in vivo or in crude extracts in the face of competition from natural amino acids. Here, we address this restriction using a pure translation system in which aminoacyl-tRNA synthetases and other competitors are deliberately omitted. First, we show that such a simplified system can synthesize long polypeptides. Second, we demonstrate pure translation display by selecting from an mRNA library only those mRNAs that encode a selectable unnatural amino acid upstream of a peptide spacer sequence long enough to span the ribosome tunnel. Pure translation display should enable the directed evolution of peptide analogs with desirable catalytic or pharmacological properties." @default.
- W2150417688 created "2016-06-24" @default.
- W2150417688 creator A5051614796 @default.
- W2150417688 creator A5053973336 @default.
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- W2150417688 date "2004-10-01" @default.
- W2150417688 modified "2023-09-25" @default.
- W2150417688 title "Pure translation display" @default.
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- W2150417688 doi "https://doi.org/10.1016/j.ab.2004.06.028" @default.
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