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- W2150428229 abstract "The poultry mite, Dermanyssus gallinae, is a parasite of fowl and birds worldwide, causing huge economic losses in production systems. We investigated gene-knockdown approach in identifying potential vaccine antigens, targeting Histamine Releasing Factor (HRF) and Cathepsin D (C), through immersion in dsRNA derived from the two genes, for 7 and 16 h respectively, alongside LacZ control. Quantitative reverse transcriptase (RT)-PCR was used to estimate the extent of gene knockdown. The results show successful knockdown of HRF target after 16 h of immersion in dsRNA. It is suggested that optimization of delivery methods for dsRNA and targeting more genes could enhance knockdown." @default.
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- W2150428229 date "2013-03-20" @default.
- W2150428229 modified "2023-09-23" @default.
- W2150428229 title "Development of an RNA-interference procedure for gene knockdown in the poultry red mite, Dermanysus gallinae : Studies on histamine releasing factor and Cathepsin-D" @default.
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