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- W2150468051 abstract "Ram spermatozoa were obtained from different regions (caput, corpus, and cauda) of the epididymis and their plasma membrane was removed using a nitrogen cavitation treatment (750 psi, 10 min equilibration at 4°C). Membrane was recovered after sucrose gradient centrifugation and identified using 125I-succinylated concanavalin A (125I-succConA) as a surface marker. Based on fluorescein isothiocyanate-succConA (FITC-succConA) labeling and electron microscopy, cavitation removed plasma membrane from the anterior sperm head in the area overlying the acrosome. Cholesterol was the major sterol in plasma membrane, with desmosterol present in sperm entering the epididymis (caput sperm) but negligible in sperm after epididymal transit (cauda sperm). Ethanolamine and choline phosphoglycerides represented 70–80% of membrane phospholipids, with the ethanolamine fraction decreasing relative to choline phosphoglycerides during epididymal transit. The molar ratio of cholesterol to phospholipid increased in the plasma membrane during maturation. The bulk phospholipid-bound fatty acids consisted primarily of palmitoyl acyl groups (16:0) in caput sperm and docosahexaenoyl acyl groups (22:6) in cauda sperm. The choline phosphoglyceride fraction was purified and analyzed. It consisted of a mixture of ether acyl glycero-3-phosphocholine and diacyl phosphoglyceride, with the dominant acyl residue, at all stages of epididymal maturation, being 22:6 throughout epididymal transit. The significance of these findings relative to acquisition of fertilization capacity by sperm during epididymal maturation is discussed." @default.
- W2150468051 created "2016-06-24" @default.
- W2150468051 creator A5070165457 @default.
- W2150468051 creator A5072598868 @default.
- W2150468051 date "1985-04-01" @default.
- W2150468051 modified "2023-09-24" @default.
- W2150468051 title "Developmental Changes Occurring in the Lipids of Ram Epididymal Spermatozoa Plasma Membrane 1" @default.
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- W2150468051 doi "https://doi.org/10.1095/biolreprod32.3.653" @default.
- W2150468051 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3158356" @default.
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