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• W2150489396 abstract "An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 × 10(6) transformants per μg DNA were achieved. The vector stably replicated in both V. vulnificus and Escherichia coli and was also successfully introduced into Vibrio parahaemolyticus and Vibrio cholerae. To demonstrate the suitability of the vector for molecular cloning, the green fluorescent protein (GFP) gene and the vvhBA hemolysin operon were inserted into the vector and functionally expressed in Vibrio and E. coli." @default.
• W2150489396 created "2016-06-24" @default.
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• W2150489396 date "2014-02-15" @default.
• W2150489396 modified "2023-09-26" @default.
• W2150489396 title "PVv3, a New Shuttle Vector for Gene Expression in Vibrio vulnificus" @default.
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• W2150489396 doi "https://doi.org/10.1128/aem.03720-13" @default.
• W2150489396 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3911060" @default.
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