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• W2150542518 abstract "To establish a robust methodology for quantitative analysis of therapeutic peptide in biological samples.Octreotide was chosen as a model therapeutic peptide, and oxidized-octreotide was synthesized as internal standard. Protein precipitation combining liquid-liquid extraction technique was adopted to enhance the recovery and reduce the endogenous interferences effectively. A LC-MS/MS method for the quantification of octreotide in plasma has been optimized and validated according to FDA guidelines.Linearity, selectivity, accuracy, precision, stability, matrix effect and recovery were within bioanalytical method validation acceptance criteria as FDA guidelines. The methodology was then successfully applied into the studies for octreotide.This robust methodology would be useful for the PK studies for octreotide and other therapeutic peptides." @default.
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• W2150542518 date "2015-04-01" @default.
• W2150542518 modified "2023-10-18" @default.
• W2150542518 title "PK study of octreotide based on LC–MS/MS combining protein precipitation and impurity extraction technique" @default.
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• W2150542518 doi "https://doi.org/10.4155/bio.15.22" @default.
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