FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2150623686> ?p ?o ?g. }

• W2150623686 endingPage "256" @default.
• W2150623686 startingPage "239" @default.
• W2150623686 abstract "Epithelial-cell enriched primary cultures have been established from rat ventral prostate (RVP). Minced ventral prostates were dissociated with 0.5% collagenase in F12K tissue culture medium containing 1% fetal bovine serum. This treatment resulted in the gradual removal of stromal elements from the base of the epithelial cells. After 60 minutes of digestion the aggregates of epithelial cells were washed and plated at high density in F12K plus 10% horse serum. After 48 hours in vitro the unattached cells were removed from the culture dishes, washed, and reinoculated into new culture vessels containing fresh medium. After 96 hours in vitro, the aggregates had attached to the culture vessels and spread out to yield discrete patches of epithelial cells. By 144 hours in vitro the patches of cells had grown and coalesced to form a semi-confluent monolayer of epithelial cells. Ultrastructural examination of these cultures indicated that adjacent cells were joined by desmosomes and tight junctions and had formed “lumen-like structures” into which projected microvilli. In addition, the cells contained secretory granules and tonofilaments, giving them a morphological appearance similar to prostate epithelial cells in the intact organ. The primary cultures also retained histochemical activities for acid phosphatase, β-glucuronidase, and succinic dehydrogenase that were similar to the intact organ." @default.
• W2150623686 created "2016-06-24" @default.
• W2150623686 creator A5033543522 @default.
• W2150623686 creator A5042471018 @default.
• W2150623686 creator A5076351708 @default.
• W2150623686 date "1980-06-01" @default.
• W2150623686 modified "2023-09-26" @default.
• W2150623686 title "Isolation, culture and characterization of epithelial cells derived from rat ventral prostate" @default.
• W2150623686 cites W1540384225 @default.
• W2150623686 cites W1900479970 @default.
• W2150623686 cites W1972860254 @default.
• W2150623686 cites W1983749823 @default.
• W2150623686 cites W1990201825 @default.
• W2150623686 cites W2006375760 @default.
• W2150623686 cites W2014288890 @default.
• W2150623686 cites W2022094318 @default.
• W2150623686 cites W2024863960 @default.
• W2150623686 cites W2029510517 @default.
• W2150623686 cites W2034912738 @default.
• W2150623686 cites W2038251588 @default.
• W2150623686 cites W2051050181 @default.
• W2150623686 cites W2058537437 @default.
• W2150623686 cites W2060279242 @default.
• W2150623686 cites W2061225879 @default.
• W2150623686 cites W2061878054 @default.
• W2150623686 cites W2084684602 @default.
• W2150623686 cites W2088244160 @default.
• W2150623686 cites W2127911529 @default.
• W2150623686 cites W2135189810 @default.
• W2150623686 cites W2137330819 @default.
• W2150623686 cites W2142596760 @default.
• W2150623686 cites W2190330961 @default.
• W2150623686 cites W2279467263 @default.
• W2150623686 cites W2399894652 @default.
• W2150623686 cites W2413926141 @default.
• W2150623686 cites W2428580134 @default.
• W2150623686 cites W4256156364 @default.
• W2150623686 doi "https://doi.org/10.1002/ar.1091970212" @default.
• W2150623686 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6251735" @default.
• W2150623686 hasPublicationYear "1980" @default.
• W2150623686 type Work @default.
• W2150623686 sameAs 2150623686 @default.
• W2150623686 citedByCount "28" @default.
• W2150623686 crossrefType "journal-article" @default.
• W2150623686 hasAuthorship W2150623686A5033543522 @default.
• W2150623686 hasAuthorship W2150623686A5042471018 @default.
• W2150623686 hasAuthorship W2150623686A5076351708 @default.
• W2150623686 hasConcept C153911025 @default.
• W2150623686 hasConcept C16930146 @default.
• W2150623686 hasConcept C181199279 @default.
• W2150623686 hasConcept C185592680 @default.
• W2150623686 hasConcept C202751555 @default.
• W2150623686 hasConcept C2778394429 @default.
• W2150623686 hasConcept C2779404806 @default.
• W2150623686 hasConcept C28483552 @default.
• W2150623686 hasConcept C31695470 @default.
• W2150623686 hasConcept C502942594 @default.
• W2150623686 hasConcept C529295009 @default.
• W2150623686 hasConcept C54355233 @default.
• W2150623686 hasConcept C55493867 @default.
• W2150623686 hasConcept C81885089 @default.
• W2150623686 hasConcept C86803240 @default.
• W2150623686 hasConcept C90703135 @default.
• W2150623686 hasConcept C95444343 @default.
• W2150623686 hasConceptScore W2150623686C153911025 @default.
• W2150623686 hasConceptScore W2150623686C16930146 @default.
• W2150623686 hasConceptScore W2150623686C181199279 @default.
• W2150623686 hasConceptScore W2150623686C185592680 @default.
• W2150623686 hasConceptScore W2150623686C202751555 @default.
• W2150623686 hasConceptScore W2150623686C2778394429 @default.
• W2150623686 hasConceptScore W2150623686C2779404806 @default.
• W2150623686 hasConceptScore W2150623686C28483552 @default.
• W2150623686 hasConceptScore W2150623686C31695470 @default.
• W2150623686 hasConceptScore W2150623686C502942594 @default.
• W2150623686 hasConceptScore W2150623686C529295009 @default.
• W2150623686 hasConceptScore W2150623686C54355233 @default.
• W2150623686 hasConceptScore W2150623686C55493867 @default.
• W2150623686 hasConceptScore W2150623686C81885089 @default.
• W2150623686 hasConceptScore W2150623686C86803240 @default.
• W2150623686 hasConceptScore W2150623686C90703135 @default.
• W2150623686 hasConceptScore W2150623686C95444343 @default.
• W2150623686 hasIssue "2" @default.
• W2150623686 hasLocation W21506236861 @default.
• W2150623686 hasLocation W21506236862 @default.
• W2150623686 hasOpenAccess W2150623686 @default.
• W2150623686 hasPrimaryLocation W21506236861 @default.
• W2150623686 hasRelatedWork W2042536217 @default.
• W2150623686 hasRelatedWork W2047542402 @default.
• W2150623686 hasRelatedWork W2090169675 @default.
• W2150623686 hasRelatedWork W2099686209 @default.
• W2150623686 hasRelatedWork W2331425169 @default.
• W2150623686 hasRelatedWork W2352277170 @default.
• W2150623686 hasRelatedWork W2367227563 @default.
• W2150623686 hasRelatedWork W2377661610 @default.
• W2150623686 hasRelatedWork W2417838062 @default.
• W2150623686 hasRelatedWork W4289992199 @default.
• W2150623686 hasVolume "197" @default.
• W2150623686 isParatext "false" @default.

• next