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- W2150647813 abstract "ABSTRACT We report here the molecular cloning and expression of a hemolytic sphingomyelinase from an aquatic bacterium, Pseudomonas sp. strain TK4. The sphingomyelinase gene was found to consist of 1,548 nucleotides encoding 516 amino acid residues. The recombinant 57.7-kDa enzyme hydrolyzed sphingomyelin but not phosphatidylcholine, phosphatidylserine, phosphatidylglycerol, phosphatidic acid, or phosphatidylethanolamine, indicating that the enzyme is a sphingomyelin-specific sphingomyelinase C. The hydrolysis of sphingomyelin by the enzyme was found to be most efficient at pH 8.0 and activated by Mn 2+ . The enzyme shows quite a broad specificity, i.e., it hydrolyzed 4-nitrobenz-2-oxa-1,3-diazole (NBD)-sphingomyelin with short-chain fatty acids and NBD-sphingosylphosphorylcholine, the latter being completely resistant to hydrolysis by any sphingomyelinase reported so far. Significant sequence similarities were found in sphingomyelinases from Bacillus cereus , Staphylococcus aureus , Listeria ivanovii , and Leptospira interrogans , as well as a hypothetical protein encoded in Chromobacterium violaceum , although the first three lacked one-third of the sequence corresponding to that from the C terminus of the TK4 enzyme. Interestingly, the deletion mutant of strain TK4 lacking 186 amino acids at the C-terminal end hydrolyzed sphingomyelin, whereas it lost all hemolytic activity, indicating that the C-terminal region of the TK4 enzyme is indispensable for the hemolytic activity." @default.
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- W2150647813 date "2002-01-15" @default.
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- W2150647813 title "Molecular Cloning and Expression of Mn <sup>2+</sup> -Dependent Sphingomyelinase/Hemolysin of an Aquatic Bacterium, <i>Pseudomonas</i> sp. Strain TK4" @default.
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- W2150647813 doi "https://doi.org/10.1128/jb.184.2.540-546.2002" @default.
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