Matches in SemOpenAlex for { <https://semopenalex.org/work/W2150659503> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2150659503 endingPage "464" @default.
- W2150659503 startingPage "459" @default.
- W2150659503 abstract "Fusogenic liposomes that incorporate Sendai virus envelope proteins, so-called Sendai virosomes, have been developed for in vitro and in vivo genetic modification of animal cells. In this study, several different virosomes of varying lipid compositions were formulated and their in vitro gene-transfer efficiencies compared. The virosomes were prepared by quantitative reconstitution of the Sendai envelope, fusion (F) and hemagglutinin-neuraminidase (HN) proteins into liposomal vesicles. Virosomes that contained luciferase reporter genes were tested in 293 transformed human kidney cells. F/HN-virosomes that were prepared with an artificial Sendai viral envelope (ASVE-virosomes) or phosphatidylserine (PS-virosomes) exhibited an 8- or 6-fold higher gene-transfer efficiency than cationic liposomes that were made with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). F/HNvirosomes that were prepared with phosphatidic acid (PA-virosomes) instead of PS were less efficient in gene transfer than either ASVE- or PS-virosomes. In addition, the gene-transfer capability of ASVE- and PS-virosomes was maximal at a Ca2+ concentration of 510 mM. These results suggest that the incorporated lipid components significantly affect the in vitro gene transfer that is mediated by Sendai F/HN-virosomes." @default.
- W2150659503 created "2016-06-24" @default.
- W2150659503 creator A5075881914 @default.
- W2150659503 creator A5089597918 @default.
- W2150659503 date "2002-09-30" @default.
- W2150659503 modified "2023-09-23" @default.
- W2150659503 title "Effect of Lipid Compositions on Gene Transfer into 293 Cells Using Sendai F/HN-virosomes" @default.
- W2150659503 cites W1966541384 @default.
- W2150659503 cites W1983326337 @default.
- W2150659503 cites W1989121066 @default.
- W2150659503 cites W1989787607 @default.
- W2150659503 cites W1995484424 @default.
- W2150659503 cites W2009474244 @default.
- W2150659503 cites W2028882586 @default.
- W2150659503 cites W2029034337 @default.
- W2150659503 cites W2045809703 @default.
- W2150659503 cites W2063057612 @default.
- W2150659503 cites W2087279904 @default.
- W2150659503 cites W2093962401 @default.
- W2150659503 cites W2095208906 @default.
- W2150659503 cites W2095454653 @default.
- W2150659503 cites W2124946399 @default.
- W2150659503 doi "https://doi.org/10.5483/bmbrep.2002.35.5.459" @default.
- W2150659503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12359086" @default.
- W2150659503 hasPublicationYear "2002" @default.
- W2150659503 type Work @default.
- W2150659503 sameAs 2150659503 @default.
- W2150659503 citedByCount "7" @default.
- W2150659503 countsByYear W21506595032012 @default.
- W2150659503 countsByYear W21506595032014 @default.
- W2150659503 crossrefType "journal-article" @default.
- W2150659503 hasAuthorship W2150659503A5075881914 @default.
- W2150659503 hasAuthorship W2150659503A5089597918 @default.
- W2150659503 hasConcept C108625454 @default.
- W2150659503 hasConcept C130316041 @default.
- W2150659503 hasConcept C153911025 @default.
- W2150659503 hasConcept C159047783 @default.
- W2150659503 hasConcept C185154212 @default.
- W2150659503 hasConcept C185592680 @default.
- W2150659503 hasConcept C2522874641 @default.
- W2150659503 hasConcept C2780127873 @default.
- W2150659503 hasConcept C41625074 @default.
- W2150659503 hasConcept C55493867 @default.
- W2150659503 hasConcept C86803240 @default.
- W2150659503 hasConcept C97380033 @default.
- W2150659503 hasConceptScore W2150659503C108625454 @default.
- W2150659503 hasConceptScore W2150659503C130316041 @default.
- W2150659503 hasConceptScore W2150659503C153911025 @default.
- W2150659503 hasConceptScore W2150659503C159047783 @default.
- W2150659503 hasConceptScore W2150659503C185154212 @default.
- W2150659503 hasConceptScore W2150659503C185592680 @default.
- W2150659503 hasConceptScore W2150659503C2522874641 @default.
- W2150659503 hasConceptScore W2150659503C2780127873 @default.
- W2150659503 hasConceptScore W2150659503C41625074 @default.
- W2150659503 hasConceptScore W2150659503C55493867 @default.
- W2150659503 hasConceptScore W2150659503C86803240 @default.
- W2150659503 hasConceptScore W2150659503C97380033 @default.
- W2150659503 hasIssue "5" @default.
- W2150659503 hasLocation W21506595031 @default.
- W2150659503 hasLocation W21506595032 @default.
- W2150659503 hasOpenAccess W2150659503 @default.
- W2150659503 hasPrimaryLocation W21506595031 @default.
- W2150659503 hasRelatedWork W1893868214 @default.
- W2150659503 hasRelatedWork W1993229826 @default.
- W2150659503 hasRelatedWork W2039204610 @default.
- W2150659503 hasRelatedWork W2043689764 @default.
- W2150659503 hasRelatedWork W2051934414 @default.
- W2150659503 hasRelatedWork W2078863565 @default.
- W2150659503 hasRelatedWork W2095454653 @default.
- W2150659503 hasRelatedWork W2158257281 @default.
- W2150659503 hasRelatedWork W2315171476 @default.
- W2150659503 hasRelatedWork W2183935793 @default.
- W2150659503 hasVolume "35" @default.
- W2150659503 isParatext "false" @default.
- W2150659503 isRetracted "false" @default.
- W2150659503 magId "2150659503" @default.
- W2150659503 workType "article" @default.