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- W2150760981 abstract "Objective: Recent studies of urine‐based enzyme‐linked immunosorbent assays (ELISA) for detection of antibody to Helicobacter pylori ( H. pylori ) have already shown high sensitivity and specificity in adults. The diagnostic accuracy of these assays in children was investigated. Methods: The results of serum and urine‐based ELISAs were compared with those of 13 C‐urea breath tests ( 13 C‐UBT) and/or detection of faecal H. pylori antigen in 68 children. The effect of urine total immunoglobulin G (IgG) levels on the ELISA results for anti‐ H. pylori antibodies in urine was also examined. Results: The sensitivity, specificity and accuracy of the serum ELISA were 72.7%, 96.3%, and 92.3% respectively, while those of the urine‐based ELISA were 92.3%, 76.4%, and 79.4% respectively. The level of urine total IgG in children with false‐positive results in the urine‐based ELISA, was significantly higher than that in children who showed negative results in both the urine‐based ELISA and the 13 C‐UBT and/or faecal H. pylori antigen tests. Human γ‐globulin affected the urine‐based ELISA results at final concentrations of 2.0 mg/dL, 3.0 mg/dL, and 4.0 mg/dL; the anti‐ H. pylori antibody values were significantly higher than the ELISA values without the addition of human γ‐globulin. Conclusion: The findings suggested that the specificity of urine‐based ELISA for detection of H. pylori antibodies is low in children, since high urinary levels of total IgG increase the likelihood of false‐positive results." @default.
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- W2150760981 date "2003-10-21" @default.
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- W2150760981 title "Urine-based enzyme-linked immunosorbent assay for the detection of Helicobacter pylori antibodies in children" @default.
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- W2150760981 doi "https://doi.org/10.1046/j.1440-1754.2003.00213.x" @default.
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