FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2150784105> ?p ?o ?g. }

• W2150784105 endingPage "674" @default.
• W2150784105 startingPage "666" @default.
• W2150784105 abstract "Abstract Posttranslational proteolytic processing of chemokines is a natural mechanism to regulate inflammation. In this study, we describe modification of the CXC chemokine stromal cell-derived factor 1α/CXCL12 by peptidylarginine deiminase (PAD) that converts arginine residues into citrulline (Cit), thereby reducing the number of positive charges. The three NH2-terminal arginines of CXCL12, Arg8, Arg12, and Arg20, were citrullinated upon incubation with PAD. The physiologic relevance of citrullination was demonstrated by showing coexpression of CXCL12 and PAD in Crohn’s disease. Three CXCL12 isoforms were synthesized for biologic characterization: CXCL12-1Cit, CXCL12-3Cit, and CXCL12-5Cit, in which Arg8, Arg8/Arg12/Arg20, or all five arginines were citrullinated, respectively. Replacement of only Arg8 caused already impaired (30-fold reduction) CXCR4 binding and signaling (calcium mobilization, phosphorylation of ERK and protein kinase B) properties. Interaction with CXCR4 was completely abolished for CXCL12-3Cit and CXCL12-5Cit. However, the CXCR7-binding capacities of CXCL12-1Cit and CXCL12-3Cit were, respectively, intact and reduced, whereas CXCL12-5Cit failed to bind CXCR7. In chemotaxis assays with lymphocytes and monocytes, CXCL12-3Cit and CXCL12-5Cit were completely devoid of activity, whereas CXCL12-1Cit, albeit at higher concentrations than CXCL12, induced migration. The antiviral potency of CXCL12-1Cit was reduced compared with CXCL12 and CXCL12-3Cit and CXCL12-5Cit (maximal dose 200 nM) could not inhibit infection of lymphocytic MT-4 cells with the HIV-1 strains NL4.3 and HE. In conclusion, modification of CXCL12 by one Cit severely impaired the CXCR4-mediated biologic effects of this chemokine and maximally citrullinated CXCL12 was inactive. Therefore, PAD is a potent physiologic down-regulator of CXCL12 function." @default.
• W2150784105 created "2016-06-24" @default.
• W2150784105 creator A5013249296 @default.
• W2150784105 creator A5017914492 @default.
• W2150784105 creator A5023796496 @default.
• W2150784105 creator A5035196127 @default.
• W2150784105 creator A5038995800 @default.
• W2150784105 creator A5045411688 @default.
• W2150784105 creator A5051941199 @default.
• W2150784105 creator A5057082439 @default.
• W2150784105 creator A5066094878 @default.
• W2150784105 creator A5067567986 @default.
• W2150784105 creator A5079381808 @default.
• W2150784105 creator A5082715265 @default.
• W2150784105 creator A5084134209 @default.
• W2150784105 date "2009-01-01" @default.
• W2150784105 modified "2023-10-05" @default.
• W2150784105 title "Citrullination of CXCL12 Differentially Reduces CXCR4 and CXCR7 Binding with Loss of Inflammatory and Anti-HIV-1 Activity via CXCR4" @default.
• W2150784105 cites W1499652269 @default.
• W2150784105 cites W1543575007 @default.
• W2150784105 cites W1572925458 @default.
• W2150784105 cites W1582204239 @default.
• W2150784105 cites W1602000691 @default.
• W2150784105 cites W1804074798 @default.
• W2150784105 cites W1963984954 @default.
• W2150784105 cites W1967211753 @default.
• W2150784105 cites W1981149366 @default.
• W2150784105 cites W1989035987 @default.
• W2150784105 cites W1990985385 @default.
• W2150784105 cites W1995187882 @default.
• W2150784105 cites W1997702183 @default.
• W2150784105 cites W2001044562 @default.
• W2150784105 cites W2007665550 @default.
• W2150784105 cites W2010638326 @default.
• W2150784105 cites W2010819027 @default.
• W2150784105 cites W2030490621 @default.
• W2150784105 cites W2031703621 @default.
• W2150784105 cites W2032031643 @default.
• W2150784105 cites W2035052662 @default.
• W2150784105 cites W2038937310 @default.
• W2150784105 cites W2046783953 @default.
• W2150784105 cites W2048430011 @default.
• W2150784105 cites W2054186705 @default.
• W2150784105 cites W2057682160 @default.
• W2150784105 cites W2061001247 @default.
• W2150784105 cites W2061860330 @default.
• W2150784105 cites W2067419441 @default.
• W2150784105 cites W2074450526 @default.
• W2150784105 cites W2075971846 @default.
• W2150784105 cites W2081769332 @default.
• W2150784105 cites W2083612980 @default.
• W2150784105 cites W2086420062 @default.
• W2150784105 cites W2090078156 @default.
• W2150784105 cites W2090476586 @default.
• W2150784105 cites W2092905357 @default.
• W2150784105 cites W2093352510 @default.
• W2150784105 cites W2102079012 @default.
• W2150784105 cites W2102096947 @default.
• W2150784105 cites W2109578241 @default.
• W2150784105 cites W2112004490 @default.
• W2150784105 cites W2128898204 @default.
• W2150784105 cites W2131112498 @default.
• W2150784105 cites W2131652492 @default.
• W2150784105 cites W2131872753 @default.
• W2150784105 cites W2132891633 @default.
• W2150784105 cites W2140654809 @default.
• W2150784105 cites W2148740102 @default.
• W2150784105 cites W2154552023 @default.
• W2150784105 cites W2156323704 @default.
• W2150784105 cites W2168936455 @default.
• W2150784105 cites W2169916808 @default.
• W2150784105 cites W2171581347 @default.
• W2150784105 cites W2171867081 @default.
• W2150784105 cites W2172005240 @default.
• W2150784105 cites W4231950505 @default.
• W2150784105 cites W4240513782 @default.
• W2150784105 cites W92043842 @default.
• W2150784105 doi "https://doi.org/10.4049/jimmunol.182.1.666" @default.
• W2150784105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19109200" @default.
• W2150784105 hasPublicationYear "2009" @default.
• W2150784105 type Work @default.
• W2150784105 sameAs 2150784105 @default.
• W2150784105 citedByCount "86" @default.
• W2150784105 countsByYear W21507841052012 @default.
• W2150784105 countsByYear W21507841052013 @default.
• W2150784105 countsByYear W21507841052014 @default.
• W2150784105 countsByYear W21507841052015 @default.
• W2150784105 countsByYear W21507841052016 @default.
• W2150784105 countsByYear W21507841052017 @default.
• W2150784105 countsByYear W21507841052018 @default.
• W2150784105 countsByYear W21507841052019 @default.
• W2150784105 countsByYear W21507841052020 @default.
• W2150784105 countsByYear W21507841052021 @default.
• W2150784105 countsByYear W21507841052022 @default.
• W2150784105 countsByYear W21507841052023 @default.
• W2150784105 crossrefType "journal-article" @default.
• W2150784105 hasAuthorship W2150784105A5013249296 @default.
• W2150784105 hasAuthorship W2150784105A5017914492 @default.

• next