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• W2150843691 abstract "ABSTRACT The terminal reaction in triacylglyceride (TAG) biosynthesis is the esterification of diacylglycerol (DAG) with a fatty acid molecule. To study this reaction in Streptomyces coelicolor , we analyzed three candidate genes (sco0958, sco1280, and sco0123) whose products significantly resemble the recently identified wax ester synthase/acyl-coenzyme A (CoA):DAG acyltransferase (DGAT) from Acinetobacter baylyi . The deletion of either sco0123 or sco1280 resulted in no detectable decrease in TAG accumulation. In contrast, the deletion of sco0958 produced a dramatic reduction in neutral lipid production, whereas the overexpression of this gene yielded a significant increase in de novo TAG biosynthesis. In vitro activity assays showed that Sco0958 mediates the esterification of DAG using long-chain acyl-CoAs (C 14 to C 18 ) as acyl donors. The K m and V max values of this enzyme for myristoyl-CoA were 45 μM and 822 nmol mg −1 min −1 , respectively. Significantly, the triple mutant strain was not completely devoid of storage lipids, indicating the existence of alternative TAG-biosynthetic routes. We present strong evidence demonstrating that the residual production of TAG in this mutant strain is mediated, at least in part, by an acyl-CoA-dependent pathway, since the triple mutant still exhibited DGAT activity. More importantly, there was substantial phospholipid:DGAT (PDAT) activity in the wild type and in the triple mutant. This is the first time that a PDAT activity has been reported for bacteria, highlighting the extreme metabolic diversity of this industrially important soil microorganism." @default.
• W2150843691 created "2016-06-24" @default.
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• W2150843691 date "2008-05-01" @default.
• W2150843691 modified "2023-10-18" @default.
• W2150843691 title "Multiple Pathways for Triacylglycerol Biosynthesis in <i>Streptomyces coelicolor</i>" @default.
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• W2150843691 doi "https://doi.org/10.1128/aem.02638-07" @default.
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