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- W2150877567 abstract "This report describes an effective method for the cloning of Mycobacterium leprae reactive T lymphocytes with Epstein-Barr-virus transformed autologous B cells as antigen-presenting cells- The two advantages of this method are that it drastically reduces the number of autologous peripheral blood mononuclear cells I (<107 cells) needed to obtain and propagate these T-cell clones (TLC). and that it enables us to expand individual TLC to large numbers of cells (<108) Thus she major obstacles for the cloning of T lymphocytes-especially important with regard to patients-are bypassed. Thus far. TLC Srom three leprosy patients have been established. These TLC are HLA class H restricted in their M. leprae directed response. A marked enhancement in antigen responsiveness was observed after further expansion of several TLC. some of which turned from nonresponder into responder TLC, Four tested TLC display strikingly different antigen recognition patterns when tested against a number of other mycobacterial antigens: one T.LC so far recognizes only M leprae antigens." @default.
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- W2150877567 date "1986-01-01" @default.
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- W2150877567 title "HLA Class-II-restricted Mycobacterium lepraereactive T-Cell Clones from Leprosy Patients Established with a Minimal Requirement for Autologous Mononuclear Cells" @default.
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- W2150877567 doi "https://doi.org/10.1111/j.1365-3083.1986.tb01947.x" @default.
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