Matches in SemOpenAlex for { <https://semopenalex.org/work/W2150932561> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2150932561 abstract "We report an extension of 3'-terminal exon trapping technology to the identification of transcribed sequences from yeast artificial chromosomes (YACs). A 350-kb YAC containing mouse genomic DNA was gel-purified and used as the target DNA for the 3'-terminal exon trapping strategy. A novel direct ligation/transfection approach was employed to increase the efficiency of trapping 3'-terminal exons from recombinant vector-derived chimeric mRNA. The resulting RT-PCR product was then used to generate a plasmid library. Randomly chosen individual subclones from this library were sequenced, and the results indicate that 86% met sequence criteria characteristic of 3'-terminal exons, whereas 14% were background from identified sources. PCR mapping efforts suggest eight putative last exons present within this YAC, whereas RT-PCR studies demonstrate that three reside within valid expressed sequences." @default.
- W2150932561 created "2016-06-24" @default.
- W2150932561 creator A5023012274 @default.
- W2150932561 creator A5031230494 @default.
- W2150932561 creator A5032713796 @default.
- W2150932561 creator A5038692278 @default.
- W2150932561 creator A5038897416 @default.
- W2150932561 creator A5044190420 @default.
- W2150932561 date "1995-06-01" @default.
- W2150932561 modified "2023-09-23" @default.
- W2150932561 title "Identification of 3'-terminal exons from yeast artificial chromosomes." @default.
- W2150932561 cites W1968504685 @default.
- W2150932561 cites W1986007518 @default.
- W2150932561 cites W1988745885 @default.
- W2150932561 cites W1989126735 @default.
- W2150932561 cites W1992544969 @default.
- W2150932561 cites W1997897487 @default.
- W2150932561 cites W2003946096 @default.
- W2150932561 cites W2008195396 @default.
- W2150932561 cites W2017734636 @default.
- W2150932561 cites W2034156530 @default.
- W2150932561 cites W2041985900 @default.
- W2150932561 cites W2045138629 @default.
- W2150932561 cites W2057051315 @default.
- W2150932561 cites W2061601461 @default.
- W2150932561 cites W2081626635 @default.
- W2150932561 cites W2165266236 @default.
- W2150932561 doi "https://doi.org/10.1101/gr.4.6.322" @default.
- W2150932561 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7580924" @default.
- W2150932561 hasPublicationYear "1995" @default.
- W2150932561 type Work @default.
- W2150932561 sameAs 2150932561 @default.
- W2150932561 citedByCount "3" @default.
- W2150932561 crossrefType "journal-article" @default.
- W2150932561 hasAuthorship W2150932561A5023012274 @default.
- W2150932561 hasAuthorship W2150932561A5031230494 @default.
- W2150932561 hasAuthorship W2150932561A5032713796 @default.
- W2150932561 hasAuthorship W2150932561A5038692278 @default.
- W2150932561 hasAuthorship W2150932561A5038897416 @default.
- W2150932561 hasAuthorship W2150932561A5044190420 @default.
- W2150932561 hasBestOaLocation W21509325611 @default.
- W2150932561 hasConcept C104317684 @default.
- W2150932561 hasConcept C122735190 @default.
- W2150932561 hasConcept C13575015 @default.
- W2150932561 hasConcept C153911025 @default.
- W2150932561 hasConcept C17757408 @default.
- W2150932561 hasConcept C194583182 @default.
- W2150932561 hasConcept C22744801 @default.
- W2150932561 hasConcept C3017666073 @default.
- W2150932561 hasConcept C30481170 @default.
- W2150932561 hasConcept C36823959 @default.
- W2150932561 hasConcept C39917842 @default.
- W2150932561 hasConcept C40767141 @default.
- W2150932561 hasConcept C51679486 @default.
- W2150932561 hasConcept C54355233 @default.
- W2150932561 hasConcept C552990157 @default.
- W2150932561 hasConcept C6970260 @default.
- W2150932561 hasConcept C86803240 @default.
- W2150932561 hasConceptScore W2150932561C104317684 @default.
- W2150932561 hasConceptScore W2150932561C122735190 @default.
- W2150932561 hasConceptScore W2150932561C13575015 @default.
- W2150932561 hasConceptScore W2150932561C153911025 @default.
- W2150932561 hasConceptScore W2150932561C17757408 @default.
- W2150932561 hasConceptScore W2150932561C194583182 @default.
- W2150932561 hasConceptScore W2150932561C22744801 @default.
- W2150932561 hasConceptScore W2150932561C3017666073 @default.
- W2150932561 hasConceptScore W2150932561C30481170 @default.
- W2150932561 hasConceptScore W2150932561C36823959 @default.
- W2150932561 hasConceptScore W2150932561C39917842 @default.
- W2150932561 hasConceptScore W2150932561C40767141 @default.
- W2150932561 hasConceptScore W2150932561C51679486 @default.
- W2150932561 hasConceptScore W2150932561C54355233 @default.
- W2150932561 hasConceptScore W2150932561C552990157 @default.
- W2150932561 hasConceptScore W2150932561C6970260 @default.
- W2150932561 hasConceptScore W2150932561C86803240 @default.
- W2150932561 hasLocation W21509325611 @default.
- W2150932561 hasLocation W21509325612 @default.
- W2150932561 hasOpenAccess W2150932561 @default.
- W2150932561 hasPrimaryLocation W21509325611 @default.
- W2150932561 hasRelatedWork W1973136168 @default.
- W2150932561 hasRelatedWork W1992619467 @default.
- W2150932561 hasRelatedWork W2001009548 @default.
- W2150932561 hasRelatedWork W2013310702 @default.
- W2150932561 hasRelatedWork W2038993942 @default.
- W2150932561 hasRelatedWork W2044403261 @default.
- W2150932561 hasRelatedWork W2057051315 @default.
- W2150932561 hasRelatedWork W2150932561 @default.
- W2150932561 hasRelatedWork W2357968955 @default.
- W2150932561 hasRelatedWork W2388334694 @default.
- W2150932561 isParatext "false" @default.
- W2150932561 isRetracted "false" @default.
- W2150932561 magId "2150932561" @default.
- W2150932561 workType "article" @default.