FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2150934761> ?p ?o ?g. }

• W2150934761 endingPage "7" @default.
• W2150934761 startingPage "4091" @default.
• W2150934761 abstract "An estrogen-responsive mouse Leydig cell tumor line (Tumor 124958) has been shown to contain only a low-affinity binder for estradiol in the cytosol fraction. This differed from the putative estrogen receptor in terms of its hormone-binding specificity as well as affinity. In addition, the possibility that an estrogen receptor-like molecule exists in the nuclei even without hormonal stimuli was examined using purified nuclei. Scatchard plot analyses showed that these nuclei possessed a large amount of estrogen binder having a high affinity for estradiol and diethylstilbestrol. The content of this nuclear binding component was not diminished by using molybdate, a potent inhibitor for receptor activation, and in vitro incubation of collagenase-dispersed cells with estradiol did not cause significant increase in the number of nuclear binding sites when compared with the values obtained by direct incubation of isolated nuclei with estradiol. These results support the view that this nuclear estrogen binder is not due to artificial migration of the cytosol receptor into nuclei during homogenization. The characterization of this nuclear binding component under cell-free conditions revealed that its affinity for estradiol in Mg2+-containing buffer was temperature dependent (Kd 3 nM at 30 degrees and 12 nM at 0 degrees) without significant alteration in the number of maximum binding sites. Introduction of a chelating agent (ethylenediaminetetraacetate) into the buffer system abolished the temperature effect on the affinity, resulting in high affinity for estradiol at both low and high temperatures. These Mg2+ and temperature effects were reversible. In addition, when compared with putative nuclear estrogen receptors, this nuclear binding was observed to be relatively resistant to high salt or micrococcal nuclease treatments in relation to solubilization from nuclei. However, trypsin digestion was found to result in a marked decrease in the nuclear binding sites, indicating that this unique nuclear binding component contains a protein unit(s). These results suggest the possibility that this tumor line contains a unique unoccupied nuclear estrogen binder which might be able to transmit estrogen signals to tumor cell nuclei with regard to tumor growth." @default.
• W2150934761 created "2016-06-24" @default.
• W2150934761 creator A5014490727 @default.
• W2150934761 creator A5014777775 @default.
• W2150934761 creator A5045586773 @default.
• W2150934761 creator A5074129968 @default.
• W2150934761 creator A5076268036 @default.
• W2150934761 creator A5082007002 @default.
• W2150934761 creator A5085783938 @default.
• W2150934761 date "1983-09-01" @default.
• W2150934761 modified "2023-09-23" @default.
• W2150934761 title "Characterization of a unique nuclear estrogen-binding component in an estrogen-responsive mouse Leydig cell tumor." @default.
• W2150934761 cites W1508586071 @default.
• W2150934761 cites W1595024935 @default.
• W2150934761 cites W1600102186 @default.
• W2150934761 cites W1604246280 @default.
• W2150934761 cites W1775749144 @default.
• W2150934761 cites W1983649348 @default.
• W2150934761 cites W1996498593 @default.
• W2150934761 cites W2002931164 @default.
• W2150934761 cites W2028234426 @default.
• W2150934761 cites W2043796829 @default.
• W2150934761 cites W2050196634 @default.
• W2150934761 cites W2062283685 @default.
• W2150934761 cites W2070030770 @default.
• W2150934761 cites W2094131386 @default.
• W2150934761 cites W2099114692 @default.
• W2150934761 cites W2127503812 @default.
• W2150934761 cites W2167965746 @default.
• W2150934761 cites W2170346923 @default.
• W2150934761 cites W2198364189 @default.
• W2150934761 cites W2278354703 @default.
• W2150934761 cites W2416696746 @default.
• W2150934761 cites W2418916358 @default.
• W2150934761 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6871850" @default.
• W2150934761 hasPublicationYear "1983" @default.
• W2150934761 type Work @default.
• W2150934761 sameAs 2150934761 @default.
• W2150934761 citedByCount "4" @default.
• W2150934761 crossrefType "journal-article" @default.
• W2150934761 hasAuthorship W2150934761A5014490727 @default.
• W2150934761 hasAuthorship W2150934761A5014777775 @default.
• W2150934761 hasAuthorship W2150934761A5045586773 @default.
• W2150934761 hasAuthorship W2150934761A5074129968 @default.
• W2150934761 hasAuthorship W2150934761A5076268036 @default.
• W2150934761 hasAuthorship W2150934761A5082007002 @default.
• W2150934761 hasAuthorship W2150934761A5085783938 @default.
• W2150934761 hasConcept C121608353 @default.
• W2150934761 hasConcept C12554922 @default.
• W2150934761 hasConcept C126322002 @default.
• W2150934761 hasConcept C134018914 @default.
• W2150934761 hasConcept C170493617 @default.
• W2150934761 hasConcept C181199279 @default.
• W2150934761 hasConcept C185592680 @default.
• W2150934761 hasConcept C202751555 @default.
• W2150934761 hasConcept C2777164284 @default.
• W2150934761 hasConcept C2781397067 @default.
• W2150934761 hasConcept C530470458 @default.
• W2150934761 hasConcept C55493867 @default.
• W2150934761 hasConcept C71924100 @default.
• W2150934761 hasConcept C84606932 @default.
• W2150934761 hasConcept C86803240 @default.
• W2150934761 hasConcept C98539663 @default.
• W2150934761 hasConceptScore W2150934761C121608353 @default.
• W2150934761 hasConceptScore W2150934761C12554922 @default.
• W2150934761 hasConceptScore W2150934761C126322002 @default.
• W2150934761 hasConceptScore W2150934761C134018914 @default.
• W2150934761 hasConceptScore W2150934761C170493617 @default.
• W2150934761 hasConceptScore W2150934761C181199279 @default.
• W2150934761 hasConceptScore W2150934761C185592680 @default.
• W2150934761 hasConceptScore W2150934761C202751555 @default.
• W2150934761 hasConceptScore W2150934761C2777164284 @default.
• W2150934761 hasConceptScore W2150934761C2781397067 @default.
• W2150934761 hasConceptScore W2150934761C530470458 @default.
• W2150934761 hasConceptScore W2150934761C55493867 @default.
• W2150934761 hasConceptScore W2150934761C71924100 @default.
• W2150934761 hasConceptScore W2150934761C84606932 @default.
• W2150934761 hasConceptScore W2150934761C86803240 @default.
• W2150934761 hasConceptScore W2150934761C98539663 @default.
• W2150934761 hasIssue "9" @default.
• W2150934761 hasLocation W21509347611 @default.
• W2150934761 hasOpenAccess W2150934761 @default.
• W2150934761 hasPrimaryLocation W21509347611 @default.
• W2150934761 hasRelatedWork W1621741447 @default.
• W2150934761 hasRelatedWork W1965298510 @default.
• W2150934761 hasRelatedWork W1996680175 @default.
• W2150934761 hasRelatedWork W1997419347 @default.
• W2150934761 hasRelatedWork W2007466509 @default.
• W2150934761 hasRelatedWork W2066318491 @default.
• W2150934761 hasRelatedWork W2071079694 @default.
• W2150934761 hasRelatedWork W2092739734 @default.
• W2150934761 hasRelatedWork W2098446053 @default.
• W2150934761 hasRelatedWork W2409769304 @default.
• W2150934761 hasVolume "43" @default.
• W2150934761 isParatext "false" @default.
• W2150934761 isRetracted "false" @default.
• W2150934761 magId "2150934761" @default.
• W2150934761 workType "article" @default.