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• W2151043708 abstract "A human ornithine decarboxylase (ODC) gene probe has been isolated from a Jurkat T-cell cDNA expression library, sequenced, and used to analyze ODC mRNA levels in untransformed human lymphocytes and fibroblasts stimulated to proliferate by various mitogens. The partial cDNA sequence is 86% homologous to the mouse ODC cDNA, and Northern blots indicate that the human and mouse mRNA species are similar in size. ODC mRNA is barely detectable in quiescent human T lymphocytes and undetectable in density-arrested WI38 fibroblasts. Following stimulation of T-lymphocyte proliferation with phytohemagglutinin, the ODC mRNA level rises to a peak around mid G1 phase and decreases as the cells enter S phase. Serum stimulation of density-arrested fibroblasts results in an elevation of the ODC mRNA level which persists throughout the cell cycle. Epidermal growth factor (20 ng/ml) but not insulin (10 mg/ml) or dexamethasone (55 ng/ml) stimulates ODC expression in quiescent WI38 fibroblasts. Southern blots suggest that human cells have a single copy of the ODC gene." @default.
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• W2151043708 date "1987-09-01" @default.
• W2151043708 modified "2023-09-25" @default.
• W2151043708 title "Cell-cycle-dependent expression of human ornithine decarboxylase" @default.
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• W2151043708 doi "https://doi.org/10.1002/jcp.1041320318" @default.
• W2151043708 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3308908" @default.
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