FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2151069820> ?p ?o ?g. }

• W2151069820 endingPage "e115206" @default.
• W2151069820 startingPage "e115206" @default.
• W2151069820 abstract "Reverse transcription-quantitative real-time PCR (RT-qPCR) is a widely used technique for gene expression analysis. The reliability of this method depends largely on the suitable selection of stable reference genes for accurate data normalization. Hypericum perforatum L. (St. John's wort) is a field growing plant that is frequently exposed to a variety of adverse environmental stresses that can negatively affect its productivity. This widely known medicinal plant with broad pharmacological properties (anti-depressant, anti-tumor, anti-inflammatory, antiviral, antioxidant, anti-cancer, and antibacterial) has been overlooked with respect to the identification of reference genes suitable for RT-qPCR data normalization. In this study, 11 candidate reference genes were analyzed in H. perforatum plants subjected to cold and heat stresses. The expression stability of these genes was assessed using GeNorm, NormFinder and BestKeeper algorithms. The results revealed that the ranking of stability among the three algorithms showed only minor differences within each treatment. The best-ranked reference genes differed between cold- and heat-treated samples; nevertheless, TUB was the most stable gene in both experimental conditions. GSA and GAPDH were found to be reliable reference genes in cold-treated samples, while GAPDH showed low expression stability in heat-treated samples. 26SrRNA and H2A had the highest stabilities in the heat assay, whereas H2A was less stable in the cold assay. Finally, AOX1, AOX2, CAT1 and CHS genes, associated with plant stress responses and oxidative stress, were used as target genes to validate the reliability of identified reference genes. These target genes showed differential expression profiles over time in treated samples. This study not only is the first systematic analysis for the selection of suitable reference genes for RT-qPCR studies in H. perforatum subjected to temperature stress conditions, but may also provide valuable information about the roles of genes associated with temperature stress responses." @default.
• W2151069820 created "2016-06-24" @default.
• W2151069820 creator A5012827680 @default.
• W2151069820 creator A5040698299 @default.
• W2151069820 creator A5053578911 @default.
• W2151069820 creator A5071667523 @default.
• W2151069820 date "2014-12-11" @default.
• W2151069820 modified "2023-09-27" @default.
• W2151069820 title "Reference Genes Selection and Normalization of Oxidative Stress Responsive Genes upon Different Temperature Stress Conditions in Hypericum perforatum L" @default.
• W2151069820 cites W1534062799 @default.
• W2151069820 cites W1577577364 @default.
• W2151069820 cites W1591807792 @default.
• W2151069820 cites W1822797627 @default.
• W2151069820 cites W1965721868 @default.
• W2151069820 cites W1967640901 @default.
• W2151069820 cites W1967946848 @default.
• W2151069820 cites W1968937403 @default.
• W2151069820 cites W1973185802 @default.
• W2151069820 cites W1974345074 @default.
• W2151069820 cites W1974517295 @default.
• W2151069820 cites W1978661454 @default.
• W2151069820 cites W1981175667 @default.
• W2151069820 cites W1984012094 @default.
• W2151069820 cites W1984648635 @default.
• W2151069820 cites W1985233328 @default.
• W2151069820 cites W1990468940 @default.
• W2151069820 cites W1994324001 @default.
• W2151069820 cites W1994669247 @default.
• W2151069820 cites W1997919181 @default.
• W2151069820 cites W2003038480 @default.
• W2151069820 cites W2004448262 @default.
• W2151069820 cites W2004736886 @default.
• W2151069820 cites W2011123092 @default.
• W2151069820 cites W2012177958 @default.
• W2151069820 cites W2016292406 @default.
• W2151069820 cites W2017161772 @default.
• W2151069820 cites W2018646107 @default.
• W2151069820 cites W2019290599 @default.
• W2151069820 cites W2019810905 @default.
• W2151069820 cites W2020799358 @default.
• W2151069820 cites W2022077450 @default.
• W2151069820 cites W2023145021 @default.
• W2151069820 cites W2024506979 @default.
• W2151069820 cites W2024556021 @default.
• W2151069820 cites W2024733819 @default.
• W2151069820 cites W2028415401 @default.
• W2151069820 cites W2030370106 @default.
• W2151069820 cites W2032392216 @default.
• W2151069820 cites W2034991062 @default.
• W2151069820 cites W2040248273 @default.
• W2151069820 cites W2043116958 @default.
• W2151069820 cites W2043881984 @default.
• W2151069820 cites W2051475076 @default.
• W2151069820 cites W2052052853 @default.
• W2151069820 cites W2052672151 @default.
• W2151069820 cites W2053386398 @default.
• W2151069820 cites W2053874089 @default.
• W2151069820 cites W2059122130 @default.
• W2151069820 cites W2063872306 @default.
• W2151069820 cites W2066821556 @default.
• W2151069820 cites W2071853384 @default.
• W2151069820 cites W2072857796 @default.
• W2151069820 cites W2073631499 @default.
• W2151069820 cites W2077479892 @default.
• W2151069820 cites W2081554497 @default.
• W2151069820 cites W2082208678 @default.
• W2151069820 cites W2082827273 @default.
• W2151069820 cites W2083274319 @default.
• W2151069820 cites W2088798237 @default.
• W2151069820 cites W2088870167 @default.
• W2151069820 cites W2092158120 @default.
• W2151069820 cites W2094710069 @default.
• W2151069820 cites W2095666435 @default.
• W2151069820 cites W2099401505 @default.
• W2151069820 cites W2099824652 @default.
• W2151069820 cites W2100516856 @default.
• W2151069820 cites W2103422169 @default.
• W2151069820 cites W2104558985 @default.
• W2151069820 cites W2105423509 @default.
• W2151069820 cites W2108998807 @default.
• W2151069820 cites W2109324504 @default.
• W2151069820 cites W2110885661 @default.
• W2151069820 cites W2111427334 @default.
• W2151069820 cites W2112982595 @default.
• W2151069820 cites W2116421717 @default.
• W2151069820 cites W2117392192 @default.
• W2151069820 cites W2120099260 @default.
• W2151069820 cites W2120239584 @default.
• W2151069820 cites W2121005257 @default.
• W2151069820 cites W2123372486 @default.
• W2151069820 cites W2125650923 @default.
• W2151069820 cites W2129261839 @default.
• W2151069820 cites W2129728705 @default.
• W2151069820 cites W2132560331 @default.
• W2151069820 cites W2138581811 @default.
• W2151069820 cites W2140933904 @default.
• W2151069820 cites W2145752548 @default.
• W2151069820 cites W2147663934 @default.

• next