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• W2151161057 abstract "In recent years, there has been an increasing interest in SSBs because they find numerous applications in diverse molecular biology and analytical methods. We report the characterization of single-stranded DNA binding proteins (SSBs) from the thermophilic bacteria Thermotoga maritima (Tma SSB) and Thermotoga neapolitana (Tne SSB). They are the smallest known bacterial SSB proteins, consisting of 141 and 142 amino acid residues with a calculated molecular mass of 16.30 and 16.58 kDa, respectively. The similarity between amino acid sequences of these proteins is very high: 90% identity and 95% similarity. Surprisingly, both Tma SSB and Tne SSB possess a quite low sequence similarity to Escherichia coli SSB (36 and 35% identity, 55 and 56% similarity, respectively). They are functional as homotetramers containing one single-stranded DNA binding domain (OB-fold) in each monomer. Agarose mobility assays indicated that the ssDNA-binding site for both proteins is salt independent, and fluorescence spectroscopy resulted in a size of 68 ± 2 nucleotides. The half-lives of Tma SSB and Tne SSB were 10 h and 12 h at 100°C, respectively. When analysed by differential scanning microcalorimetry (DSC) the melting temperature (Tm) was 109.3°C and 112.5°C for Tma SSB and Tne SSB, respectively. The results showed that Tma SSB and Tne SSB are the most thermostable SSB proteins identified to date, offering an attractive alternative to Taq SSB and Tth SSB in molecular biology applications, especially with using high temperature e. g. polymerase chain reaction (PCR)." @default.
• W2151161057 created "2016-06-24" @default.
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• W2151161057 date "2010-01-01" @default.
• W2151161057 modified "2023-09-27" @default.
• W2151161057 title "Characterization of exceptionally thermostable single-stranded DNA-binding proteins from Thermotoga maritima and Thermotoga neapolitana" @default.
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• W2151161057 doi "https://doi.org/10.1186/1471-2180-10-260" @default.
• W2151161057 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2964679" @default.
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