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• W2151288894 abstract "ABSTRACT The prevalence of active drug efflux pump and porin alterations was investigated in Turkish nosocomial strains of Klebsiella pneumoniae exhibiting a multidrug-resistant phenotype. MICs of various antibiotics, including quinolones, chloramphenicol, tetracycline, and β-lactams, for those strains were determined either with or without the efflux pump inhibitor phenylalanine arginine β-naphthylamide (PAβN). Thirty-nine percent of the strains exhibited a PAβN-modulated resistance for quinolones, chloramphenicol, and tetracycline. In these strains, a significant increase of chloramphenicol accumulation was gained in the presence of the efflux pump inhibitor PAβN or with the energy uncoupler carbonyl cyanide m -chlorophenylhydrazone. Moreover, high-level expression of the membrane fusion protein AcrA, which was immunodetected in most of those isolates, suggests that the AcrAB/TolC efflux machinery contributed to their antibiotic resistance. Studies of K. pneumoniae porins indicated that the majority of the strains, including extended-spectrum β-lactamase producers and efflux-positive ones, presented an alteration in their sorbitol-sensitive porin (OmpK35) expression. This is the first report showing the prominent role of active drug efflux in the antibiotic resistance of nosocomial K. pneumoniae strains from Turkey." @default.
• W2151288894 created "2016-06-24" @default.
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• W2151288894 date "2004-06-01" @default.
• W2151288894 modified "2023-10-14" @default.
• W2151288894 title "Detection and Prevalence of Active Drug Efflux Mechanism in Various Multidrug-Resistant <i>Klebsiella pneumoniae</i> Strains from Turkey" @default.
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• W2151288894 doi "https://doi.org/10.1128/jcm.42.6.2701-2706.2004" @default.
• W2151288894 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/427859" @default.
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