Matches in SemOpenAlex for { <https://semopenalex.org/work/W2151292854> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2151292854 endingPage "104" @default.
- W2151292854 startingPage "101" @default.
- W2151292854 abstract "Electrotransformation is a method generally used in biotechnology to introduce recombinant DNA into a wide range of bacteria. However the mechanism of DNA entry is poorly understood. We report that in Streptococcus pneumoniae, a naturally transformable species, electrotransformation efficiently introduces a plasmid replicon. DNA is strongly restricted by the restriction-modification systems DpnI and DpnII which degrade methylated and nonmethylated DNA respectively at GATC sequences. This suggests that in electrotransformation double-strand DNA penetrates into these bacteria without a single-strand step in contrast to natural transformation. Single-strand DNA by itself is able to electrotransform very weakly and linearized double-stranded plasmid DNA yields barely detectable levels of transformants." @default.
- W2151292854 created "2016-06-24" @default.
- W2151292854 creator A5017870823 @default.
- W2151292854 creator A5053043006 @default.
- W2151292854 creator A5080031131 @default.
- W2151292854 date "1997-01-01" @default.
- W2151292854 modified "2023-09-27" @default.
- W2151292854 title "Electrotransformation ofStreptococcus pneumoniae: Evidence for Restriction of the DNA on Entry" @default.
- W2151292854 cites W1587385145 @default.
- W2151292854 cites W1592912115 @default.
- W2151292854 cites W1963729325 @default.
- W2151292854 cites W1974903923 @default.
- W2151292854 cites W1977776036 @default.
- W2151292854 cites W1983101161 @default.
- W2151292854 cites W2001074737 @default.
- W2151292854 cites W2016590707 @default.
- W2151292854 cites W2022960791 @default.
- W2151292854 cites W2030954021 @default.
- W2151292854 cites W2051431720 @default.
- W2151292854 cites W2054140474 @default.
- W2151292854 cites W2062398844 @default.
- W2151292854 cites W2063635957 @default.
- W2151292854 cites W2086924423 @default.
- W2151292854 cites W2092163049 @default.
- W2151292854 cites W2142716675 @default.
- W2151292854 doi "https://doi.org/10.1089/mdr.1997.3.101" @default.
- W2151292854 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9109100" @default.
- W2151292854 hasPublicationYear "1997" @default.
- W2151292854 type Work @default.
- W2151292854 sameAs 2151292854 @default.
- W2151292854 citedByCount "5" @default.
- W2151292854 countsByYear W21512928542014 @default.
- W2151292854 countsByYear W21512928542016 @default.
- W2151292854 countsByYear W21512928542019 @default.
- W2151292854 crossrefType "journal-article" @default.
- W2151292854 hasAuthorship W2151292854A5017870823 @default.
- W2151292854 hasAuthorship W2151292854A5053043006 @default.
- W2151292854 hasAuthorship W2151292854A5080031131 @default.
- W2151292854 hasConcept C104317684 @default.
- W2151292854 hasConcept C109932267 @default.
- W2151292854 hasConcept C128319531 @default.
- W2151292854 hasConcept C153911025 @default.
- W2151292854 hasConcept C18103114 @default.
- W2151292854 hasConcept C186852380 @default.
- W2151292854 hasConcept C204241405 @default.
- W2151292854 hasConcept C205225487 @default.
- W2151292854 hasConcept C22744801 @default.
- W2151292854 hasConcept C40767141 @default.
- W2151292854 hasConcept C523546767 @default.
- W2151292854 hasConcept C54355233 @default.
- W2151292854 hasConcept C552990157 @default.
- W2151292854 hasConcept C86803240 @default.
- W2151292854 hasConcept C89423630 @default.
- W2151292854 hasConceptScore W2151292854C104317684 @default.
- W2151292854 hasConceptScore W2151292854C109932267 @default.
- W2151292854 hasConceptScore W2151292854C128319531 @default.
- W2151292854 hasConceptScore W2151292854C153911025 @default.
- W2151292854 hasConceptScore W2151292854C18103114 @default.
- W2151292854 hasConceptScore W2151292854C186852380 @default.
- W2151292854 hasConceptScore W2151292854C204241405 @default.
- W2151292854 hasConceptScore W2151292854C205225487 @default.
- W2151292854 hasConceptScore W2151292854C22744801 @default.
- W2151292854 hasConceptScore W2151292854C40767141 @default.
- W2151292854 hasConceptScore W2151292854C523546767 @default.
- W2151292854 hasConceptScore W2151292854C54355233 @default.
- W2151292854 hasConceptScore W2151292854C552990157 @default.
- W2151292854 hasConceptScore W2151292854C86803240 @default.
- W2151292854 hasConceptScore W2151292854C89423630 @default.
- W2151292854 hasIssue "1" @default.
- W2151292854 hasLocation W21512928541 @default.
- W2151292854 hasLocation W21512928542 @default.
- W2151292854 hasOpenAccess W2151292854 @default.
- W2151292854 hasPrimaryLocation W21512928541 @default.
- W2151292854 hasRelatedWork W1872531801 @default.
- W2151292854 hasRelatedWork W1883474712 @default.
- W2151292854 hasRelatedWork W1983101161 @default.
- W2151292854 hasRelatedWork W2041940794 @default.
- W2151292854 hasRelatedWork W2073875788 @default.
- W2151292854 hasRelatedWork W2090070295 @default.
- W2151292854 hasRelatedWork W2093447190 @default.
- W2151292854 hasRelatedWork W2142716675 @default.
- W2151292854 hasRelatedWork W2148366672 @default.
- W2151292854 hasRelatedWork W2348487199 @default.
- W2151292854 hasVolume "3" @default.
- W2151292854 isParatext "false" @default.
- W2151292854 isRetracted "false" @default.
- W2151292854 magId "2151292854" @default.
- W2151292854 workType "article" @default.